{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:22:25Z","timestamp":1792160545954,"version":"4.4.0"},"reference-count":79,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":170,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","award":["R01AI126897"],"award-info":[{"award-number":["R01AI126897"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1 C06 RR18386"],"award-info":[{"award-number":["1 C06 RR18386"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterial infection worldwide, potentially leading to severe pathologies including pelvic inflammatory disease, ectopic pregnancy, and tubal infertility if left untreated. Current strategies, including screening and antibiotics, have limited effectiveness due to high rates of asymptomatic cases and logistical challenges. A multiepitope prophylactic vaccine could afford long-term protection against infection. Immunoinformatic analyses were employed to design a multiepitope Chlamydia vaccine antigen. B- and T-cell epitopes from five highly conserved and immunogenic Ct antigens were predicted and selected for the vaccine design. The final construct, adjuvanted with cholera toxin A1 subunit (CTA1), was further screened for immunogenicity. CTA1-MECA (multiepitope Chlamydia trachomatis antigen) was identified as antigenic and nonallergenic. A tertiary structure was predicted, refined, and validated as a good quality model. Molecular docking exhibited strong interactions between the vaccine and toll-like receptor 4 (TLR4). Additionally, immune responses consistent with protection including IFN-\u03b3, IgG\u00a0+\u00a0IgM antibodies, and T- and B-cell responses were predicted following vaccination in an immune simulation. Expression of the construct in an Escherichia coli expression vector proved efficient. To further validate the vaccine efficacy, we assessed its immunogenicity in mice. Immunization with CTA1-MECA elicited high levels of Chlamydia-specific antibodies in mucosal and systemic compartments.<\/jats:p>","DOI":"10.1093\/femspd\/ftae015","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:36:11Z","timestamp":1718757371000},"source":"Crossref","is-referenced-by-count":4,"title":["<i>In silico<\/i> design and analysis of a multiepitope vaccine against <i>Chlamydia<\/i>"],"prefix":"10.1093","volume":"82","author":[{"given":"Tayhlor","family":"Tanner","sequence":"first","affiliation":[{"name":"Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine . 720 Westview Drive, Atlanta, GA 30310,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F N U","family":"Medhavi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine . 720 Westview Drive, Atlanta, GA 30310,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakyra","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine . 720 Westview Drive, Atlanta, GA 30310,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4759-6254","authenticated-orcid":false,"given":"Yusuf O","family":"Omosun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine . 720 Westview Drive, Atlanta, GA 30310,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis O","family":"Eko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine . 720 Westview Drive, Atlanta, GA 30310,","place":["United 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