{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T04:37:08Z","timestamp":1770784628258,"version":"3.50.0"},"reference-count":55,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":226,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"publisher","award":["2010841"],"award-info":[{"award-number":["2010841"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Australian Research Council Future Fellowship","award":["FT200100270"],"award-info":[{"award-number":["FT200100270"]}]},{"DOI":"10.13039\/100000163","name":"ARC","doi-asserted-by":"publisher","award":["DP210100362"],"award-info":[{"award-number":["DP210100362"]}],"id":[{"id":"10.13039\/100000163","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Coxiella burnetii is a globally distributed obligate intracellular pathogen. Although often asymptomatic, infections can cause acute Q fever with influenza-like symptoms and\/or severe chronic Q fever. Coxiella burnetii develops a unique replicative niche within host cells called the Coxiella-containing vacuole (CCV), facilitated by the Dot\/Icm type IV secretion system translocating a cohort of bacterial effector proteins into the host. The role of some effectors has been elucidated; however, the actions of the majority remain enigmatic and the list of true effectors is disputable. This study examined CBU2016, a unique C. burnetii protein previously designated as an effector with a role in infection. We were unable to validate CBU2016 as a translocated effector protein. Employing targeted knock-out and complemented strains, we found that the loss of CBU2016 did not cause a replication defect within Hela, THP-1, J774, or iBMDM cells or in axenic media, nor did it affect the pathogenicity of C. burnetii in the Galleria mellonella infection model. The absence of CBU2016 did, however, result in a consistent decrease in the size of CCVs in HeLa cells. These results suggest that although CBU2016 may not be a Dot\/Icm effector, it is still able to influence the host environment during infection.<\/jats:p>","DOI":"10.1093\/femspd\/ftae018","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T01:51:57Z","timestamp":1723600317000},"source":"Crossref","is-referenced-by-count":5,"title":["<i>Coxiella burnetii<\/i> protein CBU2016 supports CCV expansion"],"prefix":"10.1093","volume":"82","author":[{"given":"David R","family":"Thomas","sequence":"first","affiliation":[{"name":"Infection Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University , Clayton, VIC 3800 ,","place":["Australia"]},{"name":"Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne, VIC 3000 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah E","family":"Garnish","sequence":"additional","affiliation":[{"name":"Infection Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University , Clayton, VIC 3800 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen Ai","family":"Khoo","sequence":"additional","affiliation":[{"name":"Infection Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University , Clayton, VIC 3800 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhavna","family":"Padmanabhan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne, VIC 3000 ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nichollas E","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Microbiology and 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