{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T05:08:14Z","timestamp":1784956094147,"version":"3.55.0"},"reference-count":14,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T00:00:00Z","timestamp":1727568000000},"content-version":"vor","delay-in-days":272,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014477","name":"NHI","doi-asserted-by":"publisher","award":["5R35GM143009"],"award-info":[{"award-number":["5R35GM143009"]}],"id":[{"id":"10.13039\/100014477","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1943310"],"award-info":[{"award-number":["1943310"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neisseria gonorrhea (Ngo) is a major concern for global public health due to its severe implications for reproductive health. Understanding its metabolic phenotype is crucial for comprehending its pathogenicity. Despite Ngo\u2019s ability to encode tricarboxylic acid (TCA) cycle proteins, GltA and AcnB, their activities are notably restricted. To investigate this phenomenon, we used the iNgo_557 metabolic model and incorporated a constraint on total cellular protein content. Our results indicate that low cellular protein content severely limits GltA and AcnB activity, leading to a shift toward acetate overflow for Adenosine triphosphate (ATP) production, which is more efficient in terms of protein usage. Surprisingly, increasing cellular protein content alleviates this restriction on GltA and AcnB and delays the onset of acetate overflow, highlighting protein allocation as a critical determinant in understanding Ngo\u2019s metabolic phenotype. These findings underscore the significance of Ngo\u2019s metabolic adaptation in light of optimal protein allocation, providing a blueprint to understand Ngo\u2019s metabolic landscape.<\/jats:p>","DOI":"10.1093\/femspd\/ftae023","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T15:22:46Z","timestamp":1727450566000},"source":"Crossref","is-referenced-by-count":9,"title":["Optimal protein allocation controls the inhibition of GltA and AcnB in\n                    <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1093","volume":"82","author":[{"given":"Nabia","family":"Shahreen","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niaz Bahar","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2974-0243","authenticated-orcid":false,"given":"Rajib","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln , Lincoln, NE 68588 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,28]]},"reference":[{"key":"2024121400023000000_bib1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00625-20","article-title":"Proteome regulation patterns determine Escherichia coli wild-type and mutant phenotypes","volume":"6","author":"Alter","year":"2021","journal-title":"mSystems"},{"key":"2024121400023000000_bib2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/nature15765","article-title":"Overflow metabolism in Escherichia coli results 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