{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T20:08:23Z","timestamp":1773432503815,"version":"3.50.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":287,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"MOST","doi-asserted-by":"publisher","award":["2022YFC2304000"],"award-info":[{"award-number":["2022YFC2304000"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"MOST","doi-asserted-by":"publisher","award":["ZR2020KC006"],"award-info":[{"award-number":["ZR2020KC006"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chlamydia psittaci is threatening to the animal industry and human beings. Live attenuated duck enteritis virus (DEV) is considered a good vaccine vector. In the present study, the Pmp17G antigen of C. psittaci was expressed in DEV to construct a recombinant DEV\u2013Pmp17G vaccine. The growing curve of the rDEV\u2013Pmp17G vaccine was comparable to the parental DEV strain, and Pmp17G protein expression was detected in the cytosol and membrane of the infected host cells. A total of 30 ducklings assigned to 5 groups were used to evaluate the vaccine efficacy. The birds in the vaccine groups received 15\u2009000 plaque forming units of the rDEV\u2013Pmp17G vaccine via hypodermic injection. In contrast, the control groups received intramuscular inoculation with 1\u00a0\u00d7\u00a0103 embryo lethal dose of DEV vector or 50\u00a0\u00b5g of commercial recombinant major outer membrane protein (MOMP) vaccine. The rDEV\u2013Pmp17G vaccine induced significantly higher levels of IgG antibodies than the commercial MOMP did on day 14, and the IgG antibodies persisted for 28 days. Moreover, the rDEV\u2013Pmp17G vaccine also induced higher levels of lymphocyte proliferations compared to the DEV vector. The vaccinated animals significantly reduced lesions and enhanced bacterial clearance in the lungs and throats compared to the MOMP immunization. Thus, the rDEV\u2013Pmp17G vaccine induced persistent IgG antibodies and lymphocyte proliferation against C. psittaci infection.<\/jats:p>","DOI":"10.1093\/femspd\/ftae027","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T15:07:31Z","timestamp":1728918451000},"source":"Crossref","is-referenced-by-count":2,"title":["CRISPR\/Cas9-edited duck enteritis virus expressing Pmp17G of <i>Chlamydia psittaci<\/i> induced protective immunity in ducklings"],"prefix":"10.1093","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7179-9167","authenticated-orcid":false,"given":"Jiaqi","family":"Liu","sequence":"first","affiliation":[{"name":"National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing 100193 ,","place":["China"]},{"name":"Key Laboratory of Animal Epidemiology and Zoonoses of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China 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