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The synthesized eugenol nanoparticle (ENP) exhibited promising antibacterial and antibiofilm activity on pathogenic strain Pseudomonas aeruginosa MTCC 2488 in vitro. Here, we report on in vivo therapeutic efficacy of ENP against Pseudomonas-administered lung infection, developed in BALB\/c mice. Our findings revealed that ENP treatment significantly reduced bacterial burden in infected lung tissues and promoted recovery from infection-associated weight loss, hypothermia, and fluid accumulation in mice. In addition, histopathological and immunological studies showed that the elevated level of inflammatory cytokines TNF-\u03b1, IL-1\u03b2, MCP-1, GM-CSF, and IFN-\u03b3 in lung tissue, serum, and broncho-alveolar lavage (BAL) fluid of Pseudomonas-infected mice were considerably attenuated to almost normal level by ENP treatment. Notably, free eugenol had not so high antipseudomonal action as ENP.<\/jats:p>","DOI":"10.1093\/femspd\/ftaf014","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T12:51:40Z","timestamp":1766148700000},"source":"Crossref","is-referenced-by-count":1,"title":["Eugenol nanoparticle as a therapeutic agent against\n                    <i>Pseudomonas<\/i>\n                    infection in mice"],"prefix":"10.1093","volume":"83","author":[{"given":"Sourav","family":"Ghosh","sequence":"first","affiliation":[{"name":"University of Kalyani Department of Biochemistry and Biophysics, , Kalyani 741235, West Bengal ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3409-9830","authenticated-orcid":false,"given":"Tarakdas","family":"Basu","sequence":"additional","affiliation":[{"name":"University of Kalyani Department of Biochemistry and Biophysics, , Kalyani 741235, West Bengal 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