{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:47:34Z","timestamp":1771656454338,"version":"3.50.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T00:00:00Z","timestamp":1560470400000},"content-version":"vor","delay-in-days":74,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001775","name":"University of Technology Sydney","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Serological assays can be used to investigate the population burden of infection and potentially sequelae from Chlamydia. We investigated the PGP3 ELISA as a sero-epidemiological tool for infection or sub-fertility in Australian and Samoan women. The PGP3 ELISA absorbance levels were compared between groups of women with infertility, fertile, and current chlamydial infections. In the Australian groups, women with chlamydial tubal factor infertility had significantly higher absorbance levels in the PGP3 ELISA compared to fertile women (P\u00a0&amp;lt;\u00a00.0001), but not when compared to women with current chlamydial infection (P\u00a0=\u00a00.44). In the Samoan study, where the prevalence of chlamydial infections is much higher there were significant differences in the PGP3 ELISA absorbance levels between chlamydial sub-fertile women and fertile women (P\u00a0=\u00a00.003). There was no difference between chlamydial sub-fertile women and women with a current infection (P\u00a0=\u00a00.829). The results support that the PGP3 assay is effective for sero-epidemiological analysis of burden of infection, but not for evaluation of chlamydial pathological sequelae such as infertility.<\/jats:p>","DOI":"10.1093\/femspd\/ftz031","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T11:08:46Z","timestamp":1560251326000},"source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of a PGP3 ELISA for surveillance of the burden of <i>Chlamydia<\/i> infection in women from Australia and Samoa"],"prefix":"10.1093","volume":"77","author":[{"given":"Rami","family":"Mazraani","sequence":"first","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Ultimo, NSW 2007, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Timms","sequence":"additional","affiliation":[{"name":"Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip C","family":"Hill","sequence":"additional","affiliation":[{"name":"Centre for International Health, University of Otago, Dunedin, 9016, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamaailau","family":"Suaalii-Sauni","sequence":"additional","affiliation":[{"name":"School of Languages and Cultures, Victoria University of Wellington, Wellington, 2820, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tavita","family":"Niupulusu","sequence":"additional","affiliation":[{"name":"Samoa Cancer Society, Moto'otua Street, Apia, Samoa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiuli V A","family":"Temese","sequence":"additional","affiliation":[{"name":"Centre for Samoa Studies, National University of Samoa, Le Papaigalagala Campus, To'omatagi, Samoa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liai","family":"Iosefa-Siitia","sequence":"additional","affiliation":[{"name":"Samoa Family Health Association, Apia, Samoa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leveti","family":"Auvaa","sequence":"additional","affiliation":[{"name":"National Department of Health, Apia, Samoa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siuomatautu A","family":"Tapelu","sequence":"additional","affiliation":[{"name":"Samoa Cancer Society, Moto'otua Street, Apia, Samoa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maauga F","family":"Motu","sequence":"additional","affiliation":[{"name":"Samoan National Council of Churches, Apia, Samoa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoinette","family":"Righarts","sequence":"additional","affiliation":[{"name":"Preventive and Social Medicine, Dunedin School of Medicine, The University of Otago, Dunedin, 9016, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S","family":"Walsh","sequence":"additional","affiliation":[{"name":"Planning, Funding and Health Outcomes, Waitemata and Auckland District Health Boards, Auckland, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luk","family":"Rombauts","sequence":"additional","affiliation":[{"name":"MIMR-PH Institute of Medical Research, Monash, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A","family":"Allan","sequence":"additional","affiliation":[{"name":"UC Health Clinical School, The Wesley Hospital, Auchenflower, Queensland, 4066, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Horner","sequence":"additional","affiliation":[{"name":"Population Health Sciences, University of Bristol, Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelmina M","family":"Huston","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Ultimo, NSW 2007, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,6,14]]},"reference":[{"key":"2021052203593015800_bib1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1093\/aje\/kww117","article-title":"Proportion of tubal factor infertility due to Chlamydia: finite mixture modeling of serum antibody titers","volume":"185","author":"Ades","year":"2017","journal-title":"Am J Epidemiol"},{"key":"2021052203593015800_bib2","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1093\/humrep\/deg347","article-title":"Tubal damage in infertile women: prediction using Chlamydia serology","volume":"18","author":"Akande","year":"2003","journal-title":"Hum Reprod"},{"key":"2021052203593015800_bib3","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/BF01716696","article-title":"Chlamydia trachomatis species specific serology: immunoComb Chlamydia bivalent versus microimmunofluorescence (MIF)","volume":"22","author":"Clad","year":"1994","journal-title":"Infection"},{"key":"2021052203593015800_bib4","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1093\/humrep\/17.3.699","article-title":"Chlamydia antibody testing in screening for tubal factor subfertility: the significance of IgG antibody decline over time","volume":"17","author":"Gijsen","year":"2002","journal-title":"Hum Reprod"},{"key":"2021052203593015800_bib5","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s10096-016-2769-7","article-title":"Antibody to Chlamydia trachomatis proteins, TroA and HtrA, as a biomarker for Chlamydia trachomatis infection","volume":"36","author":"Hokynar","year":"2017","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2021052203593015800_bib6","doi-asserted-by":"crossref","first-page":"e72001","DOI":"10.1371\/journal.pone.0072001","article-title":"C. trachomatis Pgp3 antibody prevalence in young women in England, 1993\u20132010","volume":"8","author":"Horner","year":"2013","journal-title":"PLoS One"},{"key":"2021052203593015800_bib8","doi-asserted-by":"crossref","first-page":"e0151497","DOI":"10.1371\/journal.pone.0151497","article-title":"Chlamydia trachomatis Pgp3 antibody persists and correlates with self-reported infection and behavioural risks in a blinded cohort study","volume":"11","author":"Horner","year":"2016","journal-title":"PLoS One"},{"key":"2021052203593015800_bib9","doi-asserted-by":"crossref","first-page":"7503","DOI":"10.1038\/s41598-018-25918-3","article-title":"Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression","volume":"8","author":"Hufnagel","year":"2018","journal-title":"Sci Rep"},{"key":"2021052203593015800_bib10","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.jri.2010.02.007","article-title":"HtrA, RseP, and Tsp proteins do not elicit a pathology-related serum IgG response during sexually transmitted infection with Chlamydia trachomatis","volume":"85","author":"Huston","year":"2010","journal-title":"J Reprod Immunol"},{"key":"2021052203593015800_bib11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/humupd\/dmp035","article-title":"Epidemiology of Chlamydia trachomatisinfection in women and the cost-effectiveness of screening","volume":"16","author":"Land","year":"2010","journal-title":"Human Reprod Update"},{"key":"2021052203593015800_bib12","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1099\/jmm.0.000311","article-title":"Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis related infertility in women","volume":"65","author":"Menon","year":"2016","journal-title":"J Med Microbiol"},{"key":"2021052203593015800_bib14","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1186\/s12879-016-1508-0","article-title":"Sero-epidemiological assessment of Chlamydia trachomatis infection and sub-fertility in Samoan women","volume":"16","author":"Menon","year":"2016","journal-title":"Bmc Infect Dis"},{"key":"2021052203593015800_bib13","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1128\/CMR.00035-15","article-title":"Human and pathogen factors associated with Chlamydia trachomatis-related infertility in women","volume":"28","author":"Menon","year":"2015","journal-title":"Clin Microbiol Rev"},{"key":"2021052203593015800_bib15","doi-asserted-by":"crossref","first-page":"e00253","DOI":"10.1128\/mSphere.00253-18","article-title":"Comprehensive molecular serology of human Chlamydia trachomatis infections by peptide enzyme-linked immunosorbent assays","volume":"3","author":"Rahman","year":"2018","journal-title":"mSphere"},{"key":"2021052203593015800_bib16","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.fertnstert.2011.06.021","article-title":"Genome-wide identification of Chlamydia trachomatis antigens associated with tubal factor infertility","volume":"96","author":"Rodgers","year":"2011","journal-title":"Fertil Steril"},{"key":"2021052203593015800_bib17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.rinim.2013.05.001","article-title":"Proof of concept: a bioinformatic and serological screening method for identifying new peptide antigens for Chlamydia trachomatis related sequelae in women","volume":"3","author":"Stansfield","year":"2013","journal-title":"Results in Immuno"},{"key":"2021052203593015800_bib18","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/trstmh\/trv014","article-title":"Prevalence of Chlamydia trachomatis infection in Samoan women aged 18 to 29 and assessment of possible risk factors: a community-based study","volume":"109","author":"Walsh","year":"2015","journal-title":"Trans Royal Soc Trop Med Hygiene"},{"key":"2021052203593015800_bib19","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1128\/CVI.00021-09","article-title":"Pgp3 antibody enzyme-linked immunosorbent assay, a sensitive and specific assay for seroepidemiological analysis of Chlamydia trachomatisinfection","volume":"16","author":"Wills","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"2021052203593015800_bib20","doi-asserted-by":"crossref","first-page":"e0152810","DOI":"10.1371\/journal.pone.0152810","article-title":"Chlamydia trachomatis Pgp3 antibody population seroprevalence before and during an era of widespread opportunistic Chlamydia screening in England (1994\u20132012)","volume":"12","author":"Woodhall","year":"2017","journal-title":"PLoS One"}],"container-title":["Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femspd\/advance-article-pdf\/doi\/10.1093\/femspd\/ftz031\/28832231\/ftz031.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femspd\/article-pdf\/77\/3\/ftz031\/38097434\/ftz031.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femspd\/article-pdf\/77\/3\/ftz031\/38097434\/ftz031.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T04:01:43Z","timestamp":1621656103000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femspd\/article\/doi\/10.1093\/femspd\/ftz031\/5519228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,1]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/femspd\/ftz031","relation":{},"ISSN":["2049-632X"],"issn-type":[{"value":"2049-632X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,4]]},"published":{"date-parts":[[2019,4,1]]},"article-number":"ftz031"}}