{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T16:02:25Z","timestamp":1776182545343,"version":"3.50.1"},"reference-count":80,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["IN214917"],"award-info":[{"award-number":["IN214917"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"DGAPA, UNAM","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Ustilago maydis is a Basidiomycota fungus, in which very little is known about its mechanisms of cell survival and death. To date, only the role of metacaspase1, acetate and hydrogen peroxide as inducers of cell death has been investigated.<\/jats:p>\n               <jats:p>In the present work, we analyzed the lifespan of U. maydis compared with other species like Sporisorium reilianum, Saccharomyces cerevisiae and Yarrowia lipolytica, and we observed that U. maydis has a minor lifespan.<\/jats:p>\n               <jats:p>We probe the addition of low concentrations metformin and curcumin to the culture media, and we observed that both prolonged the lifespan of U. maydis, a result observed for the first time in a phytopathogen fungus. However, higher concentrations of curcumin were toxic for the cells, and interestingly induced the yeast-to-mycelium dimorphic transition. The positive effect of metformin and curcumin appears to be related to an inhibition of the mechanistic Target of Rapamycin (mTOR) pathway, increase expression of autophagy genes and reducing of reactive oxygen species.<\/jats:p>\n               <jats:p>These data indicate that U. maydis may be a eukaryotic model organism to elucidate the molecular mechanism underlying apoptotic and necrosis pathways, and the lifespan increase caused by metformin and curcumin.<\/jats:p>","DOI":"10.1093\/femsyr\/foaa051","type":"journal-article","created":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T11:14:30Z","timestamp":1600168470000},"source":"Crossref","is-referenced-by-count":7,"title":["Cell death in <i>Ustilago maydis:<\/i> comparison with other fungi and the effect of metformin and curcumin on its chronological lifespan"],"prefix":"10.1093","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3856-4365","authenticated-orcid":false,"given":"Cinthia V","family":"Soberanes-Guti\u00e9rrez","sequence":"first","affiliation":[{"name":"Laboratorio de Ciencias Agrogen\u00f3micas, de la Escuela Nacional de Estudios Superiores Unidad Le\u00f3n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Blv. UNAM 2011, Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, Le\u00f3n Gto., M\u00e9xico"},{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica, Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Km. 9.6 Libramiento Norte Carr. Irapuato-Le\u00f3n 36824 Irapuato Gto., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Le\u00f3n-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica, Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Km. 9.6 Libramiento Norte Carr. Irapuato-Le\u00f3n 36824 Irapuato Gto., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lino","family":"S\u00e1nchez-Segura","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica, Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Km. 9.6 Libramiento Norte Carr. Irapuato-Le\u00f3n 36824 Irapuato Gto., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Cordero-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio de Ciencias Agrogen\u00f3micas, de la Escuela Nacional de Estudios Superiores Unidad Le\u00f3n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Blv. UNAM 2011, Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, Le\u00f3n Gto., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio C","family":"Vega-Arregu\u00edn","sequence":"additional","affiliation":[{"name":"Laboratorio de Ciencias Agrogen\u00f3micas, de la Escuela Nacional de Estudios Superiores Unidad Le\u00f3n, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Blv. UNAM 2011, Col. Predio el Saucillo y El Potrero, Comunidad de Los Tepetates, 37684, Le\u00f3n Gto., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Ruiz-Herrera","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica, Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Km. 9.6 Libramiento Norte Carr. Irapuato-Le\u00f3n 36824 Irapuato Gto., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,9,18]]},"reference":[{"key":"2021071004293687800_bib1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/B978-0-08-047378-9.50013-0","article-title":"Environmental effects on the development of infectious plant disease","volume-title":"Plant Pathology","author":"Agrios","year":"2005","edition":"5th edn"},{"key":"2021071004293687800_bib2","doi-asserted-by":"crossref","first-page":"945","DOI":"10.18632\/aging.100245","article-title":"Gender differences in metformin effect on aging, life span and spontaneous tumorigenesis in 129\/Sv mice","volume":"2","author":"Anisimov","year":"2010","journal-title":"Aging (Albany NY)"},{"key":"2021071004293687800_bib3","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/s41420-019-0180-8","article-title":"Curcumin induced oxidative stress causes autophagy and apoptosis in bovine leucocytes transformed by Theileria annulata","volume":"5","author":"Araveti","year":"2019","journal-title":"Cell Death Discov"},{"key":"2021071004293687800_bib4","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.cmet.2016.05.011","article-title":"Metformin as a tool to target aging","volume":"23","author":"Barzilai","year":"2016","journal-title":"Cell Metab"},{"key":"2021071004293687800_bib5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s10863-006-9003-8","article-title":"The ROS production induced by a reverse-electron flux at respiratory-chain complex 1 is hampered by metformin","volume":"38","author":"Batandier","year":"2006","journal-title":"J Bioenerg Biomembr"},{"key":"2021071004293687800_bib6","first-page":"757","article-title":"Curcumin inhibits the mammalian target of rapamycin-mediated signaling pathways in cancer cells","volume":"119","author":"Beevers","year":"2006","journal-title":"IJC"},{"key":"2021071004293687800_bib7","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1086\/337906","article-title":"A factor that promotes mycelial development in Sporisorium reilianum in vitro","volume":"152","author":"Bhaskaran","year":"1991","journal-title":"Bot Gaz"},{"key":"2021071004293687800_bib8","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.3390\/ijms20051239","article-title":"The role of curcumin in the modulation of ageing","volume":"20","author":"Bielak-Zmijewska","year":"2019","journal-title":"Int J Mol"},{"key":"2021071004293687800_bib9","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1042\/BJ20140620","article-title":"Effects of metformin and other biguanides on oxidative phosphorylation in mitochondria","volume":"462","author":"Bridges","year":"2014","journal-title":"Biochem J"},{"key":"2021071004293687800_bib10","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.cell.2013.02.035","article-title":"Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism","volume":"153","author":"Cabreiro","year":"2013","journal-title":"Cell"},{"key":"2021071004293687800_bib11","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.fgb.2012.07.005","article-title":"Metamorphosis of the basidiomycota Ustilago maydis: transformation of yeast-like cells into basidiocarps","volume":"49","author":"Cabrera-Ponce","year":"2012","journal-title":"Fungal Genet Biol"},{"key":"2021071004293687800_bib12","doi-asserted-by":"crossref","first-page":"e31268","DOI":"10.7554\/eLife.31268","article-title":"Metformin extends C. elegans lifespan through lysosomal pathway","volume":"6","author":"Chen","year":"2017","journal-title":"Elife"},{"key":"2021071004293687800_bib13","doi-asserted-by":"crossref","first-page":"8145","DOI":"10.1038\/srep08145","article-title":"Antihyperglycemic mechanism of metformin occurs via the AMPK\/LXR\u03b1\/POMC pathway","volume":"5","author":"Cho","year":"2015","journal-title":"Sci Rep"},{"key":"2021071004293687800_bib14","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1126\/science.1173635","article-title":"Caloric restriction delays disease onset and mortality in rhesus monkeys","volume":"325","author":"Colman","year":"2009","journal-title":"Science"},{"key":"2021071004293687800_bib15","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0092-8674(04)00046-7","article-title":"Cell death: critical control points","volume":"116","author":"Danial","year":"2004","journal-title":"Cell"},{"key":"2021071004293687800_bib16","doi-asserted-by":"crossref","first-page":"E2501","DOI":"10.1073\/pnas.1321776111","article-title":"Metformin promotes lifespan through mitohormesis via the peroxiredoxin PRDX-2","volume":"111","author":"De\u00a0Haes","year":"2014","journal-title":"Proc Natl Acad Sci"},{"key":"2021071004293687800_bib17","doi-asserted-by":"crossref","first-page":"10804","DOI":"10.1158\/0008-5472.CAN-07-2310","article-title":"Metformin inhibits mammalian target of rapamycin\u2013dependent translation initiation in breast cancer cells","volume":"67","author":"Dowling","year":"2007","journal-title":"Cancer Res"},{"key":"2021071004293687800_bib18","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.canlet.2004.09.041","article-title":"Chemopreventive and therapeutic effects of curcumin","volume":"223","author":"Duvoix","year":"2005","journal-title":"Cancer Lett"},{"key":"2021071004293687800_bib19","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.ceb.2004.09.011","article-title":"Death by design: apoptosis, necrosis and autophagy","volume":"16","author":"Edinger","year":"2004","journal-title":"Curr Opin Cell Biol"},{"key":"2021071004293687800_bib20","doi-asserted-by":"crossref","first-page":"7719","DOI":"10.1038\/sj.onc.1203249","article-title":"More than one way to die: apoptosis, necrosis and reactive oxygen damage","volume":"18","author":"Fiers","year":"1999","journal-title":"Oncogene"},{"key":"2021071004293687800_bib21","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1126\/science.1172539","article-title":"Extending healthy life span from yeast to humans","volume":"328","author":"Fontana","year":"2010","journal-title":"Science"},{"key":"2021071004293687800_bib22","first-page":"494","article-title":"Pheromone-induced G2 arrest in the phytopathogenic fungus Ustilago maydis. Eukaryot","volume":"2","author":"Garc\u00eda-Muse","year":"2003","journal-title":"Cell"},{"key":"2021071004293687800_bib23","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.2337\/dc10-0666","article-title":"Diabetes and cancer: a consensus report","volume":"33","author":"Giovannucci","year":"2010","journal-title":"Diabet Care"},{"key":"2021071004293687800_bib24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev.phyto.43.040204.135923","article-title":"Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens","volume":"43","author":"Glazebrook","year":"2005","journal-title":"Annu Rev Phytopathol"},{"key":"2021071004293687800_bib25","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.tibs.2006.11.001","article-title":"Cell death by necrosis: towards a molecular definition","volume":"32","author":"Golstein","year":"2007","journal-title":"Trends Biochem Sci"},{"key":"2021071004293687800_bib26","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/S1043-4666(05)80003-1","article-title":"Cell membrane permeabilization and cellular collapse, followed by loss of dehydrogenase activity: early events in tumour necrosis factor-induced cytotoxicity","volume":"5","author":"Grooten","year":"1993","journal-title":"Cytokine"},{"key":"2021071004293687800_bib27","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1017\/S0016672300000719","article-title":"The genetics of Ustilago maydis","volume":"2","author":"Holliday","year":"1961","journal-title":"Genet Res"},{"key":"2021071004293687800_bib28","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.cmet.2010.03.014","article-title":"Metformin, independent of AMPK, inhibits mTORC1 in a rag GTPase-dependent manner","volume":"11","author":"Kalender","year":"2010","journal-title":"Cell Metab"},{"key":"2021071004293687800_bib29","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1242\/jcs.01620","article-title":"The molecular machinery of autophagy: unanswered questions","volume":"118","author":"Klionsky","year":"2005","journal-title":"J Cell Sci"},{"key":"2021071004293687800_bib30","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/mmi.13894","article-title":"Acetate provokes mitochondrial stress and cell death in Ustilago maydis","volume":"107","author":"Kretschmer","year":"2018","journal-title":"Mol Microbiol"},{"key":"2021071004293687800_bib31","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/cdd.2008.150","article-title":"Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009","volume":"16","author":"Kroemer","year":"2009","journal-title":"Cell Death Differ"},{"key":"2021071004293687800_bib32","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/sj.cdd.4402060","article-title":"Caspase function in programmed cell death","volume":"14","author":"Kumar","year":"2007","journal-title":"Cell Death Differ"},{"key":"2021071004293687800_bib33","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/j.1749-6632.2001.tb05658.x","article-title":"Caloric restriction in primates","volume":"928","author":"Lane","year":"2001","journal-title":"Ann N Y Acad Sci"},{"key":"2021071004293687800_bib34","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1038\/nrmicro.2017.33","article-title":"Ustilago maydis effectors and their impact on virulence","volume":"15","author":"Lanver","year":"2017","journal-title":"Nat Rev Microbiol"},{"key":"2021071004293687800_bib35","doi-asserted-by":"crossref","first-page":"8977","DOI":"10.1073\/pnas.1201689109","article-title":"Distinct perturbation of the translatome by the antidiabetic drug metformin","volume":"109","author":"Larsson","year":"2012","journal-title":"Proc Natl Acad Sci"},{"key":"2021071004293687800_bib36","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1089\/rej.2010.1031","article-title":"Curcumin extends life span, improves health span, and modulates the expression of age-associated aging genes in Drosophila melanogaster","volume":"13","author":"Lee","year":"2010","journal-title":"Rejuvenation Res"},{"key":"2021071004293687800_bib37","doi-asserted-by":"crossref","first-page":"13348","DOI":"10.1073\/pnas.1006610107","article-title":"Metacaspase Yca1 is required for clearance of insoluble protein aggregates","volume":"107","author":"Lee","year":"2010","journal-title":"Proc Natl Acad Sci"},{"key":"2021071004293687800_bib38","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.mad.2011.07.008","article-title":"Curcumin-mediated lifespan extension in Caenorhabditis elegans","volume":"132","author":"Liao","year":"2011","journal-title":"Mech Ageing Dev"},{"key":"2021071004293687800_bib39","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.4161\/cc.10.17.16359","article-title":"Potent anti-proliferative effects of metformin on trastuzumab-resistant breast cancer cells via inhibition of erbB2\/IGF-1 receptor interactions","volume":"10","author":"Liu","year":"2011","journal-title":"Cell Cycle"},{"key":"2021071004293687800_bib40","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2021071004293687800_bib41","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1083\/jcb.139.3.729","article-title":"A yeast mutant showing diagnostic markers of early and late apoptosis","volume":"139","author":"Madeo","year":"1997","journal-title":"J Cell Biol"},{"key":"2021071004293687800_bib42","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/S1097-2765(02)00501-4","article-title":"A caspase-related protease regulates apoptosis in yeast","volume":"9","author":"Madeo","year":"2002","journal-title":"Mol Cell"},{"key":"2021071004293687800_bib43","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1016\/j.lfs.2005.12.007","article-title":"Multiple biological activities of curcumin: a short review","volume":"78","author":"Maheshwari","year":"2006","journal-title":"Life Sci"},{"key":"2021071004293687800_bib44","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.18632\/aging.101250","article-title":"Effects of resveratrol, curcumin, berberine and other nutraceuticals on aging, cancer development, cancer stem cells and microRNAs","volume":"9","author":"McCubrey","year":"2017","journal-title":"Aging (Albany NY)"},{"key":"2021071004293687800_bib45","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-0-387-46401-5_3","article-title":"Antioxidant and anti-inflammatory properties of curcumin","volume-title":"The Molecular Targets and Therapeutic Uses of Curcumin in Health and Disease","author":"Menon","year":"2007"},{"key":"2021071004293687800_bib46","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/mmi.13848","article-title":"Induction of apoptosis-like cell death and clearance of stress-induced intracellular protein aggregates: dual roles for Ustilago maydis metacaspase Mca1","volume":"106","author":"Mukherjee","year":"2017","journal-title":"Mol Microbiol"},{"key":"2021071004293687800_bib47","first-page":"463","article-title":"The autophagy genes atg8 and atg1 affect morphogenesis and pathogenicity in Ustilago maydis. Mol","volume":"11","author":"Nadal","year":"2010","journal-title":"Plant Pathol"},{"key":"2021071004293687800_bib48","doi-asserted-by":"crossref","first-page":"27692","DOI":"10.1074\/jbc.M114.592576","article-title":"Mechanism of metformin-dependent Inhibition of Mammalian Target of Rapamycin (mTOR) and Ras activity in pancreatic cancer role of specificity protein (Sp) transcription factors","volume":"289","author":"Nair","year":"2014","journal-title":"J Biol Chem"},{"key":"2021071004293687800_bib49","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1002\/art.20267","article-title":"Chromatin breakdown during necrosis by serum Dnase1 and the plasminogen system","volume":"50","author":"Napirei","year":"2004","journal-title":"Arth Rheumat Off J Am Col Rheumatol"},{"key":"2021071004293687800_bib50","doi-asserted-by":"crossref","first-page":"e8758","DOI":"10.1371\/journal.pone.0008758","article-title":"Metformin induces a dietary restriction\u2013like state and the oxidative stress response to extend C. elegans healthspan via AMPK, LKB1, and SKN-1","volume":"5","author":"Onken","year":"2010","journal-title":"PLoS One"},{"key":"2021071004293687800_bib51","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3390\/biom7030059","article-title":"Coupling TOR to the cell cycle by the Greatwall-Endosulfine-PP2A-B55 pathway","volume":"7","author":"P\u00e9rez-Hidalgo","year":"2017","journal-title":"Biomolecules"},{"key":"2021071004293687800_bib52","first-page":"1","article-title":"Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis","author":"Romero-Aguilar","year":"2020","journal-title":"Arch Microbiol"},{"key":"2021071004293687800_bib53","doi-asserted-by":"crossref","first-page":"4366","DOI":"10.1158\/0008-5472.CAN-10-1769","article-title":"Metformin, independent of AMPK, induces mTOR inhibition and cell-cycle arrest through REDD1","volume":"71","author":"Sahra","year":"2011","journal-title":"Cancer Res"},{"key":"2021071004293687800_bib54","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1093\/ecam\/nem043","article-title":"Curcumin in cell death processes: a challenge for CAM of age-related pathologies","volume":"4","author":"Salvioli","year":"2007","journal-title":"Evid Based Complement Alternat Med"},{"key":"2021071004293687800_bib55","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.cell.2010.02.024","article-title":"Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids","volume":"141","author":"Sancak","year":"2010","journal-title":"Cell"},{"key":"2021071004293687800_bib56","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1126\/science.1157535","article-title":"The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1","volume":"320","author":"Sancak","year":"2008","journal-title":"Science"},{"key":"2021071004293687800_bib57","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat Methods"},{"key":"2021071004293687800_bib58","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/biof.1086","article-title":"Curcumin and aging","volume":"39","author":"Shen","year":"2013","journal-title":"Biofactors"},{"key":"2021071004293687800_bib59","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1007\/s11357-012-9438-2","article-title":"Curcumin-supplemented diets increase superoxide dismutase activity and mean lifespan in Drosophila","volume":"35","author":"Shen","year":"2013","journal-title":"Age"},{"key":"2021071004293687800_bib60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1742-4933-7-1","article-title":"Curcumin, inflammation, ageing and age-related diseases","volume":"7","author":"Sikora","year":"2010","journal-title":"Immun Ageing"},{"key":"2021071004293687800_bib61","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1007\/s00253-010-2824-8","article-title":"Effects of calorie restriction on life span of microorganisms","volume":"88","author":"Skinner","year":"2010","journal-title":"Appl Microbiol Biotechnol"},{"key":"2021071004293687800_bib62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0047699","article-title":"Activation of AMPK by the putative dietary restriction mimetic metformin is insufficient to extend lifespan in Drosophila","volume":"7","author":"Slack","year":"2012","journal-title":"PLoS One"},{"key":"2021071004293687800_bib63","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1093\/gerona\/glq033","article-title":"Metformin supplementation and life span in Fischer-344 rats","volume":"65","author":"Smith","year":"2010","journal-title":"J Gerontol A Biol Sci Med Sci"},{"key":"2021071004293687800_bib64","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/00275514.1992.12026126","article-title":"Sporidial fusion and infection of maize seedlings by the smut fungus Ustilago maydis","volume":"84","author":"Snetselaar","year":"1992","journal-title":"Mycologia"},{"key":"2021071004293687800_bib65","doi-asserted-by":"crossref","first-page":"fnz108","DOI":"10.1093\/femsle\/fnz108","article-title":"Autophagosomes accumulation in the vacuoles of the fungus Ustilago maydis and the role of proteases in their digestion","volume":"366","author":"Soberanes-Guti\u00e9rrez","year":"2019","journal-title":"FEMS Microbiol Lett"},{"key":"2021071004293687800_bib66","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.exger.2012.09.007","article-title":"Curcumin is an early-acting stage-specific inducer of extended functional longevity in Drosophila","volume":"48","author":"Soh","year":"2013","journal-title":"Exp Gerontol"},{"key":"2021071004293687800_bib67","first-page":"1","article-title":"Impact of curcumin on replicative and chronological aging in the Saccharomyces cerevisiae yeast","author":"St\u0119pie\u0144","year":"2020","journal-title":"Biogerontology"},{"key":"2021071004293687800_bib68","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1083\/jcb.119.2.301","article-title":"Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction","volume":"119","author":"Takeshige","year":"1992","journal-title":"J Cell Biol"},{"key":"2021071004293687800_bib69","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3390\/biom7040077","article-title":"Evolutionary conservation of the components in the TOR signaling pathways","volume":"7","author":"Tatebe","year":"2017","journal-title":"Biomolecules"},{"key":"2021071004293687800_bib70","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1248\/cpb.33.1725","article-title":"Natural antioxidants. III. Antioxidative components isolated from rhizome of Curcuma longa L","volume":"33","author":"ToDA","year":"1985","journal-title":"Chem Pharm Bull"},{"key":"2021071004293687800_bib71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/cddis.2011.86","article-title":"Metformin inhibits melanoma development through autophagy and apoptosis mechanisms","volume":"2","author":"Tomic","year":"2011","journal-title":"Cell Death Dis"},{"key":"2021071004293687800_bib72","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1039\/C7FO01242J","article-title":"Curcumin as a functional food-derived factor: degradation products, metabolites, bioactivity, and future perspectives","volume":"9","author":"Tsuda","year":"2018","journal-title":"Food Funct"},{"key":"2021071004293687800_bib73","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1046\/j.1471-4159.2001.00679.x","article-title":"Implications of CAD and DNase II in ischemic neuronal necrosis specific for the primate hippocampus","volume":"79","author":"Tsukada","year":"2001","journal-title":"J Neurochem"},{"key":"2021071004293687800_bib74","first-page":"3703","article-title":"Isolation and characterization of an autonomously replicating sequence from Ustilago maydis","volume":"8","author":"Tsukuda","year":"1988","journal-title":"Mol Cell Biol"},{"key":"2021071004293687800_bib75","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.bbagen.2005.09.008","article-title":"Inhibition of lipid peroxidation and protein oxidation in rat liver mitochondria by curcumin and its analogues","volume":"1760","author":"Wei","year":"2006","journal-title":"Biochim Biophys Acta (BBA)-Gen Sub"},{"key":"2021071004293687800_bib76","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.4161\/auto.7.10.16740","article-title":"AMP-activated protein kinase modulates cardiac autophagy in diabetic cardiomyopathy","volume":"7","author":"Xie","year":"2011","journal-title":"Autophagy"},{"key":"2021071004293687800_bib77","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.4161\/auto.26058","article-title":"Regulation of PIK3C3\/VPS34 complexes by MTOR in nutrient stress-induced autophagy","volume":"9","author":"Yuan","year":"2013","journal-title":"Autophagy"},{"key":"2021071004293687800_bib78","first-page":"767","article-title":"A rapid method for the determination of hydrogen peroxide concentration","volume":"34","author":"Zhang","year":"2014","journal-title":"Spectrosc Spect Anal"},{"key":"2021071004293687800_bib79","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.2147\/TCRM.S141738","article-title":"Curcumin inhibits apoptosis by modulating Bax\/Bcl-2 expression and alleviates oxidative stress in testes of streptozotocin-induced diabetic rats","volume":"13","author":"Zhao","year":"2017","journal-title":"Ther Clin Risk Manag"},{"key":"2021071004293687800_bib80","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1172\/JCI13505","article-title":"Role of AMP-activated protein kinase in mechanism of metformin action","volume":"108","author":"Zhou","year":"2001","journal-title":"The J Clin Invest"}],"container-title":["FEMS Yeast Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsyr\/advance-article-pdf\/doi\/10.1093\/femsyr\/foaa051\/33768700\/foaa051.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsyr\/article-pdf\/20\/7\/foaa051\/38887715\/foaa051.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsyr\/article-pdf\/20\/7\/foaa051\/38887715\/foaa051.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T07:04:02Z","timestamp":1625900642000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsyr\/article\/doi\/10.1093\/femsyr\/foaa051\/5908381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,18]]},"references-count":80,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,10,15]]}},"URL":"https:\/\/doi.org\/10.1093\/femsyr\/foaa051","relation":{},"ISSN":["1567-1356","1567-1364"],"issn-type":[{"value":"1567-1356","type":"print"},{"value":"1567-1364","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,11]]},"published":{"date-parts":[[2020,9,18]]},"article-number":"foaa051"}}