{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:36:07Z","timestamp":1792161367008,"version":"4.4.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["528\/17\u20131"],"award-info":[{"award-number":["528\/17\u20131"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Although less prevalent than its relative Candida albicans, the yeast Candida glabrata is a successful pathogen of humans, which causes life-threatening candidiasis. It is thus vital to understand the pathogenicity mechanisms and contributing genes in C. glabrata. However, gene complementation as a tool for restoring the function of a previously deleted gene is not standardized in C. glabrata, and it is less frequently used than in C. albicans.<\/jats:p><jats:p>In this study, we established a gene complementation strategy using genomic integration at the TRP1 locus. We prove that our approach can not only be used for integration of complementation cassettes, but also for overexpression of markers like fluorescent proteins and the antigen ovalbumin, or of potential pathogenicity-related factors like the biotin transporter gene VHT1. With urea amidolyase Dur1,2 as an example, we demonstrate the application of the gene complementation approach for the expression of sequence-modified genes. With this approach, we found that a lysine-to-arginine mutation in the biotinylation motif of Dur1,2 impairs urea-dependent growth of C. glabrata and C. albicans. Taken together, the TRP1-based gene complementation approach is a valuable tool for investigating novel gene functions and for elucidating their role in the pathobiology of C. glabrata.<\/jats:p>","DOI":"10.1093\/femsyr\/foaa066","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T21:25:44Z","timestamp":1607117144000},"source":"Crossref","is-referenced-by-count":4,"title":["A<i>TRP1<\/i>-marker-based system for gene complementation, overexpression, reporter gene expression and gene modification in<i>Candida glabrata<\/i>"],"prefix":"10.1093","volume":"20","author":[{"given":"Marcel","family":"Sprenger","sequence":"first","affiliation":[{"name":"Department Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Adolf-Reichwein-Stra\u00dfe 23, 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Brunke","sequence":"additional","affiliation":[{"name":"Department Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Adolf-Reichwein-Stra\u00dfe 23, 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Hube","sequence":"additional","affiliation":[{"name":"Department Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Adolf-Reichwein-Stra\u00dfe 23, 07745 Jena, Germany"},{"name":"Institute of Microbiology, Friedrich Schiller University, Neugasse 25, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4552-7063","authenticated-orcid":false,"given":"Lydia","family":"Kasper","sequence":"additional","affiliation":[{"name":"Department Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Adolf-Reichwein-Stra\u00dfe 23, 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"2021010612201652400_bib1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/yea.3019","article-title":"Candida glabrata: a deadly companion?","volume":"31","author":"Bolotin-Fukuhara","year":"2014","journal-title":"Yeast"},{"key":"2021010612201652400_bib2","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1128\/EC.3.4.900-909.2004","article-title":"Ectopic expression of URA3 can influence the virulence phenotypes and proteome of Candida albicans but can be overcome by targeted reintegration of URA3 at the RPS10 locus","volume":"3","author":"Brand","year":"2004","journal-title":"Eukaryot Cell"},{"key":"2021010612201652400_bib3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/j.1432-1033.1985.tb08988.x","article-title":"Nucleotide sequence of the Escherichia coli gap gene. Different evolutionary behavior of the NAD+-binding domain and of the catalytic domain of D-glyceraldehyde-3-phosphate dehydrogenase","volume":"150","author":"Branlant","year":"1985","journal-title":"Eur J Biochem"},{"key":"2021010612201652400_bib4","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1242\/dmm.019901","article-title":"Of mice, flies\u2013and men? Comparing fungal infection models for large-scale screening efforts","volume":"8","author":"Brunke","year":"2015","journal-title":"Dis Model Mech"},{"key":"2021010612201652400_bib5","doi-asserted-by":"crossref","first-page":"477S","DOI":"10.1093\/jn\/129.2.477S","article-title":"Molecular biology of biotin attachment to proteins","volume":"129","author":"Chapman-Smith","year":"1999","journal-title":"J Nutr"},{"key":"2021010612201652400_bib6","doi-asserted-by":"crossref","first-page":"e1002777","DOI":"10.1371\/journal.ppat.1002777","article-title":"Candida albicans scavenges host zinc via Pra1 during endothelial invasion","volume":"8","author":"Citiulo","year":"2012","journal-title":"PLoS Pathog"},{"key":"2021010612201652400_bib7","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1099\/00221287-143-2-303","article-title":"Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans","volume":"143(Pt 2)","author":"Cormack","year":"1997","journal-title":"Microbiology"},{"key":"2021010612201652400_bib9","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1093\/genetics\/162.4.1573","article-title":"Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis","volume":"162","author":"Davis","year":"2002","journal-title":"Genetics"},{"key":"2021010612201652400_bib10","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.diagmicrobio.2012.02.001","article-title":"The changing epidemiology of healthcare-associated candidemia over three decades","volume":"73","author":"Diekema","year":"2012","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021010612201652400_bib11","doi-asserted-by":"crossref","first-page":"S274","DOI":"10.1093\/cid\/10.Supplement_2.S274","article-title":"Molecular Koch's postulates applied to microbial pathogenicity","volume":"10 Suppl 2","author":"Falkow","year":"1988","journal-title":"Rev Infect Dis"},{"key":"2021010612201652400_bib12","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1046\/j.1365-2958.2002.03166.x","article-title":"Modular domain structure in the Candida glabrata adhesin Epa1p, a beta1,6 glucan-cross-linked cell wall protein","volume":"46","author":"Frieman","year":"2002","journal-title":"Mol Microbiol"},{"key":"2021010612201652400_bib13","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/fov110","article-title":"The birth of a deadly yeast: tracing the evolutionary emergence of virulence traits in Candida glabrata","volume":"16","author":"Gabald\u00f3n","year":"2016","journal-title":"FEMS Yeast Res"},{"key":"2021010612201652400_bib14","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20092345","article-title":"Divergent Approaches to Virulence in C. albicans and C. glabrata: Two Sides of the Same Coin","volume":"20","author":"Galocha","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2021010612201652400_bib15","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1534\/genetics.112.145243","article-title":"The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae","volume":"203","author":"Gartenberg","year":"2016","journal-title":"Genetics"},{"key":"2021010612201652400_bib16","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1099\/mic.0.064097-0","article-title":"Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans","volume":"159","author":"Gerami-Nejad","year":"2013","journal-title":"Microbiology"},{"key":"2021010612201652400_bib18","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1128\/IAI.01452-08","article-title":"Arginine-induced germ tube formation in Candida albicans is essential for escape from murine macrophage line RAW 264.7","volume":"77","author":"Ghosh","year":"2009","journal-title":"Infect Immun"},{"key":"2021010612201652400_bib19","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1002\/yea.1044","article-title":"New modules for PCR-based gene targeting in Candida albicans: rapid and efficient gene targeting using 100\u00a0bp of flanking homology region","volume":"20","author":"Gola","year":"2003","journal-title":"Yeast"},{"key":"2021010612201652400_bib20","doi-asserted-by":"crossref","first-page":"e00281","DOI":"10.1128\/IAI.00281-18","article-title":"Brucella Peptide Cross-Reactive Major Histocompatibility Complex Class I Presentation Activates SIINFEKL-Specific T Cell Receptor-Expressing T Cells","volume":"86","author":"Harms","year":"2018","journal-title":"Infect Immun"},{"key":"2021010612201652400_bib21","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/1567-1364.12043","article-title":"The heme-binding protein Dap1 links iron homeostasis to azole resistance via the P450 protein Erg11 in Candida glabrata","volume":"13","author":"Hosogaya","year":"2013","journal-title":"FEMS Yeast Res"},{"key":"2021010612201652400_bib22","doi-asserted-by":"crossref","first-page":"e1003479","DOI":"10.1371\/journal.pcbi.1003479","article-title":"A virtual infection model quantifies innate effector mechanisms and Candida albicans immune escape in human blood","volume":"10","author":"H\u00fcnniger","year":"2014","journal-title":"PLoS Comput Biol"},{"key":"2021010612201652400_bib23","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.4049\/jimmunol.0901607","article-title":"Quantitating T cell cross-reactivity for unrelated peptide antigens","volume":"183","author":"Ishizuka","year":"2009","journal-title":"J Immunol"},{"key":"2021010612201652400_bib24","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1271\/bbb.69.755","article-title":"Structural characteristics of hen egg ovalbumin expressed in yeast Pichia pastoris","volume":"69","author":"Ito","year":"2005","journal-title":"Biosci Biotechnol Biochem"},{"key":"2021010612201652400_bib25","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1128\/IAI.01244-09","article-title":"Candida glabrata persistence in mice does not depend on host immunosuppression and is unaffected by fungal amino acid auxotrophy","volume":"78","author":"Jacobsen","year":"2010","journal-title":"Infect Immun"},{"key":"2021010612201652400_bib26","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1038\/s41435-019-0071-2","article-title":"Studying fungal pathogens of humans and fungal infections: fungal diversity and diversity of approaches","volume":"20","author":"Janbon","year":"2019","journal-title":"Genes Immun"},{"key":"2021010612201652400_bib27","doi-asserted-by":"crossref","first-page":"4260","DOI":"10.1038\/s41467-018-06607-1","article-title":"The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes","volume":"9","author":"Kasper","year":"2018","journal-title":"Nat Commun"},{"key":"2021010612201652400_bib28","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0378-1119(96)00132-1","article-title":"Isolation of a Candida glabrata centromere and its use in construction of plasmid vectors","volume":"175","author":"Kitada","year":"1996","journal-title":"Gene"},{"key":"2021010612201652400_bib29","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1146\/annurev.bi.58.070189.001211","article-title":"The mechanism of biotin-dependent enzymes","volume":"58","author":"Knowles","year":"1989","journal-title":"Annu Rev Biochem"},{"key":"2021010612201652400_bib30","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.4049\/jimmunol.1301332","article-title":"Candida-elicited murine Th17 cells express high Ctla-4 compared with Th1 cells and are resistant to costimulation blockade","volume":"192","author":"Krummey","year":"2014","journal-title":"J Immunol"},{"key":"2021010612201652400_bib31","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S1567-1356(03)00012-6","article-title":"Phylogenetic relationships among yeasts of the \u2018Saccharomyces complex\u2019 determined from multigene sequence analyses","volume":"3","author":"Kurtzman","year":"2003","journal-title":"FEMS Yeast Res"},{"key":"2021010612201652400_bib32","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1016\/S0021-9258(18)93826-3","article-title":"The Enzymatic Synthesis of Holotranscarboxylase from Apotranscarboxylase and (+)-Biotin. Ii. Investigation of the Reaction Mechanism","volume":"239","author":"Lane","year":"1964","journal-title":"J Biol Chem"},{"key":"2021010612201652400_bib33","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/978-1-61779-539-8_28","article-title":"Isolation and amplification of fungal RNA for microarray analysis from host samples","volume":"845","author":"L\u00fcttich","year":"2012","journal-title":"Methods Mol Biol"},{"key":"2021010612201652400_bib34","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1128\/JCM.32.4.1115-1118.1994","article-title":"Fluconazole-resistant recurrent oral candidiasis in human immunodeficiency virus-positive patients: persistence of Candida albicans strains with the same genotype","volume":"32","author":"Millon","year":"1994","journal-title":"J Clin Microbiol"},{"key":"2021010612201652400_bib35","doi-asserted-by":"crossref","first-page":"e52850","DOI":"10.1371\/journal.pone.0052850","article-title":"Cellular responses of Candida albicans to phagocytosis and the extracellular activities of neutrophils are critical to counteract carbohydrate starvation, oxidative and nitrosative stress","volume":"7","author":"Miram\u00f3n","year":"2012","journal-title":"PLoS One"},{"key":"2021010612201652400_bib36","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/j.1567-1364.2011.00734.x","article-title":"The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata","volume":"11","author":"Miyazaki","year":"2011","journal-title":"FEMS Yeast Res"},{"key":"2021010612201652400_bib37","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/s004380050665","article-title":"Expression of a chromosomally integrated, single-copy GFP gene in Candida albicans, and its use as a reporter of gene regulation","volume":"257","author":"Morschh\u00e4user","year":"1998","journal-title":"Mol Gen Genet"},{"key":"2021010612201652400_bib38","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1002\/1097-0061(20000315)16:4<325::AID-YEA538>3.0.CO;2-#","article-title":"CIp10, an efficient and convenient integrating vector for Candida albicans","volume":"16","author":"Murad","year":"2000","journal-title":"Yeast"},{"key":"2021010612201652400_bib39","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1099\/mic.0.045005-0","article-title":"Dur3 is the major urea transporter in Candida albicans and is co-regulated with the urea amidolyase Dur1,2","volume":"157","author":"Navarathna","year":"2011","journal-title":"Microbiology"},{"key":"2021010612201652400_bib40","doi-asserted-by":"crossref","first-page":"e48475","DOI":"10.1371\/journal.pone.0048475","article-title":"Urea amidolyase (DUR1,2) contributes to virulence and kidney pathogenesis of Candida albicans","volume":"7","author":"Navarathna","year":"2012","journal-title":"PLoS One"},{"key":"2021010612201652400_bib41","doi-asserted-by":"crossref","first-page":"e1001322","DOI":"10.1371\/journal.ppat.1001322","article-title":"Host iron withholding demands siderophore utilization for Candida glabrata to survive macrophage killing","volume":"7","author":"Nevitt","year":"2011","journal-title":"PLoS Pathog"},{"key":"2021010612201652400_bib42","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1128\/AAC.01278-12","article-title":"STB5 is a negative regulator of azole resistance in Candida glabrata","volume":"57","author":"Noble","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"2021010612201652400_bib43","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/0378-1119(83)90040-9","article-title":"Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis","volume":"26","author":"Norrander","year":"1983","journal-title":"Gene"},{"key":"2021010612201652400_bib44","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"2021010612201652400_bib45","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1002\/(SICI)1097-0061(199612)12:16<1677::AID-YEA79>3.0.CO;2-U","article-title":"Understanding Candida albicans at the molecular level","volume":"12","author":"Pla","year":"1996","journal-title":"Yeast"},{"key":"2021010612201652400_bib46","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1534\/genetics.111.132266","article-title":"Chromatin and transcription in yeast","volume":"190","author":"Rando","year":"2012","journal-title":"Genetics"},{"key":"2021010612201652400_bib47","doi-asserted-by":"crossref","first-page":"4107","DOI":"10.1016\/S0021-9258(19)45046-1","article-title":"Urea amidolyase. I. Properties of the enzyme from Candida utilis","volume":"247","author":"Roon","year":"1972","journal-title":"J Biol Chem"},{"key":"2021010612201652400_bib48","doi-asserted-by":"crossref","first-page":"6461","DOI":"10.1016\/S0021-9258(18)68661-2","article-title":"Evolutionary conservation among biotin enzymes","volume":"263","author":"Samols","year":"1988","journal-title":"J Biol Chem"},{"key":"2021010612201652400_bib49","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1128\/AAC.40.10.2300","article-title":"Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors","volume":"40","author":"Sanglard","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"2021010612201652400_bib50","doi-asserted-by":"crossref","first-page":"e1004211","DOI":"10.1371\/journal.ppat.1004211","article-title":"Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes","volume":"10","author":"Schwarzm\u00fcller","year":"2014","journal-title":"PLoS Pathog"},{"key":"2021010612201652400_bib51","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1128\/EC.00262-13","article-title":"Immune evasion, stress resistance, and efficient nutrient acquisition are crucial for intracellular survival of Candida glabrata within macrophages","volume":"13","author":"Seider","year":"2014","journal-title":"Eukaryot Cell"},{"key":"2021010612201652400_bib52","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.13197","article-title":"Fungal biotin homeostasis is essential for immune evasion after macrophage phagocytosis and virulence","author":"Sprenger","year":"2020","journal-title":"Cell Microbiol"},{"key":"2021010612201652400_bib53","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.1099\/mic.0.26445-0","article-title":"Integrative, multifunctional plasmids for hypha-specific or constitutive expression of green fluorescent protein in Candida albicans","volume":"149","author":"Staab","year":"2003","journal-title":"Microbiology"},{"key":"2021010612201652400_bib54","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.tibs.2017.02.001","article-title":"Mechanisms Governing Precise Protein Biotinylation","volume":"42","author":"Sternicki","year":"2017","journal-title":"Trends Biochem Sci"},{"key":"2021010612201652400_bib55","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1007\/s00018-012-1096-0","article-title":"Structure and function of biotin-dependent carboxylases","volume":"70","author":"Tong","year":"2013","journal-title":"Cell Mol Life Sci"},{"key":"2021010612201652400_bib56","doi-asserted-by":"crossref","first-page":"e24759","DOI":"10.1371\/journal.pone.0024759","article-title":"Intestinal resident yeast Candida glabrata requires Cyb2p-mediated lactate assimilation to adapt in mouse intestine","volume":"6","author":"Ueno","year":"2011","journal-title":"PLoS One"},{"key":"2021010612201652400_bib57","doi-asserted-by":"crossref","first-page":"e26962","DOI":"10.1371\/journal.pone.0026962","article-title":"In vivo systematic analysis of Candida albicans Zn2-Cys6 transcription factors mutants for mice organ colonization","volume":"6","author":"Vandeputte","year":"2011","journal-title":"PLoS One"},{"key":"2021010612201652400_bib58","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s00294-002-0349-0","article-title":"An improved transformation protocol for the human fungal pathogen Candida albicans","volume":"42","author":"Walther","year":"2003","journal-title":"Curr Genet"},{"key":"2021010612201652400_bib60","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1128\/JB.181.6.1868-1874.1999","article-title":"Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions","volume":"181","author":"Wilson","year":"1999","journal-title":"J Bacteriol"},{"key":"2021010612201652400_bib61","doi-asserted-by":"crossref","first-page":"507","DOI":"10.4161\/viru.28893","article-title":"Molecular genetic techniques for gene manipulation in Candida albicans","volume":"5","author":"Xu","year":"2014","journal-title":"Virulence"},{"key":"2021010612201652400_bib62","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.fgb.2015.04.020","article-title":"Expression vectors for C-terminal fusions with fluorescent proteins and epitope tags in Candida glabrata","volume":"80","author":"Y\u00e1nez-Carrillo","year":"2015","journal-title":"Fungal Genet Biol"},{"key":"2021010612201652400_bib63","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/biof.8","article-title":"Biotin","volume":"35","author":"Zempleni","year":"2009","journal-title":"Biofactors"},{"key":"2021010612201652400_bib64","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1534\/g3.113.006908","article-title":"Expression plasmids for use in Candida glabrata","volume":"3","author":"Zordan","year":"2013","journal-title":"G3 (Bethesda)"}],"container-title":["FEMS Yeast Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsyr\/advance-article-pdf\/doi\/10.1093\/femsyr\/foaa066\/34797733\/foaa066.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsyr\/article-pdf\/20\/8\/foaa066\/35472345\/foaa066.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsyr\/article-pdf\/20\/8\/foaa066\/35472345\/foaa066.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T13:04:38Z","timestamp":1669986278000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsyr\/article\/doi\/10.1093\/femsyr\/foaa066\/6027539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":61,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,1,6]]}},"URL":"https:\/\/doi.org\/10.1093\/femsyr\/foaa066","relation":{},"ISSN":["1567-1364"],"issn-type":[{"value":"1567-1364","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,12]]},"published":{"date-parts":[[2020,12]]},"article-number":"foaa066"}}