{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T01:10:49Z","timestamp":1776215449742,"version":"3.50.1"},"reference-count":77,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T00:00:00Z","timestamp":1636502400000},"content-version":"vor","delay-in-days":9,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2012\/11524\u20139"],"award-info":[{"award-number":["2012\/11524\u20139"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2011\/07365\u20131"],"award-info":[{"award-number":["2011\/07365\u20131"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2013\/07937\u20138"],"award-info":[{"award-number":["2013\/07937\u20138"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013724","name":"CEPF","doi-asserted-by":"publisher","award":["2017\/09443\u20133"],"award-info":[{"award-number":["2017\/09443\u20133"]}],"id":[{"id":"10.13039\/100013724","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013724","name":"CEPF","doi-asserted-by":"publisher","award":["2013\/00586\u20135"],"award-info":[{"award-number":["2013\/00586\u20135"]}],"id":[{"id":"10.13039\/100013724","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["457851\/2014\u20137"],"award-info":[{"award-number":["457851\/2014\u20137"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["051\/2013"],"award-info":[{"award-number":["051\/2013"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Coenzyme Q (CoQ) is an essential molecule that consists of a highly substituted benzene ring attached to a polyprenyl tail anchored in the inner mitochondrial membrane. CoQ transfers electrons from NADH dehydrogenase and succinate dehydrogenase complexes toward ubiquinol-cytochrome c reductase, and that allows aerobic growth of cells. In Saccharomyces cerevisiae, the synthesis of CoQ depends on fourteen proteins Coq1p-Co11p, Yah1p, Arh1p, and Hfd1p. Some of these proteins are components of CoQ synthome. Using ab initio molecular modeling and site-directed mutagenesis, we identified the functional residues of the O-methyltransferase Coq3p, which depends on S-adenosylmethionine for catalysis and is necessary for two O-methylation steps required for CoQ maturation. Conserved residues as well as those that coevolved in the protein structure were found to have important roles in respiratory growth, CoQ biosynthesis, and also in the stability of CoQ synthome proteins. Finally, a multiple sequence alignment showed that S. cerevisiae Coq3p has a 45 amino acid residues insertion that is poorly conserved or absent in oleaginous yeast, cells that can store up to 20% of their dry weight as lipids. These results point to the Coq3p structural determinants of its biological and catalytic function and could contribute to the development of lipid-producing yeast for biotechnology.<\/jats:p>","DOI":"10.1093\/femsyr\/foab055","type":"journal-article","created":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T08:19:06Z","timestamp":1636100346000},"source":"Crossref","is-referenced-by-count":2,"title":["Coq3p relevant residues for protein activity and stability"],"prefix":"10.1093","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9486-9337","authenticated-orcid":false,"given":"Janaina A","family":"Paulela","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo , Av. Prof. Lineu Prestes, 1374 \u2013 Butant\u00e3 \u2013 S\u00e3o Paulo-SP \u2013 CEP 05508-000,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4642-9729","authenticated-orcid":false,"given":"Fernando","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Biologia Evolutiva, Instituto de Bioci\u00eancias, Universidade de S\u00e3o Paulo , Rua do Mat\u00e3o, travessa 14, n\u00famero 321, Cidade Universit\u00e1ria, S\u00e3o Paulo-SP, CEP 05508-090,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2642-0444","authenticated-orcid":false,"given":"Vittoria de Lima","family":"Camandona","sequence":"additional","affiliation":[{"name":"Escola de Artes, Ci\u00eancias e Humanidades, Universidade de S\u00e3o Paulo , Rua Arlindo Bettio, 1000 - Vila Guaraciaba - S\u00e3o Paulo-SP - CEP 03828-000,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3086-4059","authenticated-orcid":false,"given":"Thiago G P","family":"Alegria","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Biologia Evolutiva, Instituto de Bioci\u00eancias, Universidade de S\u00e3o Paulo , Rua do Mat\u00e3o, travessa 14, n\u00famero 321, Cidade Universit\u00e1ria, S\u00e3o Paulo-SP, CEP 05508-090,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4250-9177","authenticated-orcid":false,"given":"Luis E S","family":"Netto","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Biologia Evolutiva, Instituto de Bioci\u00eancias, Universidade de S\u00e3o Paulo , Rua do Mat\u00e3o, travessa 14, n\u00famero 321, Cidade Universit\u00e1ria, S\u00e3o Paulo-SP, CEP 05508-090,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7121-4952","authenticated-orcid":false,"given":"Lucas","family":"Bleicher","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia \u2013 Instituto de Ci\u00eancias Biol\u00f3gicas \u2013 Universidade Federal de Minas Gerais , Av. Ant\u00f4nio Carlos, 6627 - Campus Pampulha - Belo Horizonte-MG CEP 31270-901,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2977-9815","authenticated-orcid":false,"given":"Mario H","family":"Barros","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo , Av. Prof. Lineu Prestes, 1374 \u2013 Butant\u00e3 \u2013 S\u00e3o Paulo-SP \u2013 CEP 05508-000,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5539-5298","authenticated-orcid":false,"given":"Jose Ribamar","family":"Ferreira-Junior","sequence":"additional","affiliation":[{"name":"Escola de Artes, Ci\u00eancias e Humanidades, Universidade de S\u00e3o Paulo , Rua Arlindo Bettio, 1000 - Vila Guaraciaba - S\u00e3o Paulo-SP - CEP 03828-000,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,11,10]]},"reference":[{"key":"2026041420155530600_bib1","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.bbabio.2016.03.036","article-title":"Coenzyme Q biosynthesis in health and disease","volume":"1857","author":"Acosta","year":"2016","journal-title":"Biochimica et Biophysica Acta (BBA) - Bioenergetics"},{"key":"2026041420155530600_bib2","doi-asserted-by":"crossref","first-page":"7517","DOI":"10.1074\/jbc.M114.633131","article-title":"Identification of Coq11, a new coenzyme Q biosynthetic protein in the CoQ-synthome in Saccharomyces cerevisiae","volume":"290","author":"Allan","year":"2015","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib3","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1042\/EBC20170106","article-title":"Coenzyme Q10 deficiencies: pathways in yeast and humans","volume":"62","author":"Awad","year":"2018","journal-title":"Essays Biochem"},{"key":"2026041420155530600_bib4","doi-asserted-by":"crossref","first-page":"102127","DOI":"10.1016\/j.redox.2021.102127","article-title":"Genetic screening reveals phospholipid metabolism as a key regulator of the biosynthesis of the redox-active lipid coenzyme Q","volume":"46","author":"Ayer","year":"2021","journal-title":"Redox Biol"},{"key":"2026041420155530600_bib5","doi-asserted-by":"crossref","first-page":"42627","DOI":"10.1074\/jbc.M510768200","article-title":"The Saccharomyces cerevisiae COQ10 gene encodes a START domain protein required for function of coenzyme Q in respiration","volume":"280","author":"Barros","year":"2005","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib6","doi-asserted-by":"crossref","first-page":"e27786","DOI":"10.1371\/journal.pone.0027786","article-title":"Using amino acid correlation and community detection algorithms to identify functional determinants in protein families","volume":"6","author":"Bleicher","year":"2011","journal-title":"PLoS One"},{"key":"2026041420155530600_bib7","doi-asserted-by":"crossref","first-page":"6023","DOI":"10.1074\/jbc.RA119.012420","article-title":"COQ11 deletion mitigates respiratory deficiency caused by mutations in the gene encoding the coenzyme Q chaperone protein Coq10","volume":"295","author":"Bradley","year":"2020","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.biochi.2015.10.016","article-title":"Coq7p relevant residues for protein activity and stability","volume":"119","author":"Busso","year":"2015","journal-title":"Biochimie"},{"key":"2026041420155530600_bib9","doi-asserted-by":"crossref","first-page":"16636","DOI":"10.1016\/S0021-9258(18)55349-7","article-title":"Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase gene","volume":"266","author":"Clarke","year":"1991","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib10","doi-asserted-by":"crossref","first-page":"7534","DOI":"10.1073\/pnas.93.15.7534","article-title":"Enhanced sensitivity of ubiquinone-deficient mutants of Saccharomyces cerevisiae to products of autoxidized polyunsaturated fatty acids","volume":"93","author":"Do","year":"1996","journal-title":"Proc Natl Acad Sci"},{"key":"2026041420155530600_bib11","doi-asserted-by":"crossref","first-page":"47250","DOI":"10.18632\/oncotarget.16872","article-title":"The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis","volume":"8","author":"Fan","year":"2017","journal-title":"Oncotarget"},{"key":"2026041420155530600_bib12","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.fgb.2013.06.006","article-title":"Her2p molecular modeling, mutant analysis and intramitochondrial localization","volume":"60","author":"Ferreira-Junior","year":"2013","journal-title":"Fungal Genet Biol"},{"key":"2026041420155530600_bib13","doi-asserted-by":"crossref","first-page":"D279","DOI":"10.1093\/nar\/gkv1344","article-title":"The Pfam protein families database: towards a more sustainable future","volume":"44","author":"Finn","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"2026041420155530600_bib14","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1089\/cmb.2017.0181","article-title":"PFstats: a Network-Based open tool for protein family analysis","volume":"25","author":"Fonseca-Junior","year":"2018","journal-title":"J Comput Biol"},{"key":"2026041420155530600_bib15","first-page":"304","article-title":"Human mitochondrial pathologies of the respiratory chain and ATP synthase: contributions from studies of saccharomyces cerevisiae","volume":"10","author":"Franco","year":"2020","journal-title":"Life (Basel)"},{"key":"2026041420155530600_bib16","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1074\/mcp.M114.043083","article-title":"MitoFates: improved prediction of mitochondrial targeting sequences and their cleavage sites","volume":"14","author":"Fukasawa","year":"2015","journal-title":"Mol Cell Proteomics"},{"key":"2026041420155530600_bib17","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1002\/iub.1243","article-title":"Regulation of coenzyme Q biosynthesis in yeast: a new complex in the block","volume":"66","author":"Gonzalez-Mariscal","year":"2014","journal-title":"IUBMB Life"},{"key":"2026041420155530600_bib18","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/cbin.10913","article-title":"Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits","volume":"42","author":"Guedes-Monteiro","year":"2018","journal-title":"Cell Biol Int"},{"key":"2026041420155530600_bib19","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.chembiol.2018.12.001","article-title":"A Soluble metabolon synthesizes the isoprenoid lipid ubiquinone","volume":"26","author":"Hajj\u00a0Chehade","year":"2019","journal-title":"Cell Chemical Biology"},{"key":"2026041420155530600_bib20","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1038\/nprot.2007.132","article-title":"Gene splicing and mutagenesis by PCR-driven overlap extension","volume":"2","author":"Heckman","year":"2007","journal-title":"Nat Protoc"},{"key":"2026041420155530600_bib21","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1002\/yea.320020304","article-title":"Yeast\/E. coli shuttle vectors with multiple unique restriction sites","volume":"2","author":"Hill","year":"1986","journal-title":"Yeast"},{"key":"2026041420155530600_bib22","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/S0969-2126(01)00643-8","article-title":"Two polymorphic forms of human histamine methyltransferase: structural, thermal, and kinetic comparisons","volume":"9","author":"Horton","year":"2001","journal-title":"Structure"},{"key":"2026041420155530600_bib23","doi-asserted-by":"crossref","first-page":"D884","DOI":"10.1093\/nar\/gkaa942","article-title":"Ensembl 2021","volume":"49","author":"Howe","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2026041420155530600_bib24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.abb.2007.02.016","article-title":"Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex","volume":"463","author":"Hsieh","year":"2007","journal-title":"Arch Biochem Biophys"},{"key":"2026041420155530600_bib25","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S1388-1981(00)00019-6","article-title":"Genetic evidence for a multi-subunit complex in the O-methyltransferase steps of coenzyme Q biosynthesis","volume":"1484","author":"Hsu","year":"2000","journal-title":"Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids"},{"key":"2026041420155530600_bib26","doi-asserted-by":"crossref","first-page":"9797","DOI":"10.1021\/bi9602932","article-title":"Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis","volume":"35","author":"Hsu","year":"1996","journal-title":"Biochemistry"},{"key":"2026041420155530600_bib27","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nature08433","article-title":"Structural insights into mechanisms of the small RNA methyltransferase HEN1","volume":"461","author":"Huang","year":"2009","journal-title":"Nature"},{"key":"2026041420155530600_bib28","doi-asserted-by":"crossref","first-page":"31397","DOI":"10.1074\/jbc.M503277200","article-title":"COQ9, a new gene required for the biosynthesis of coenzyme Q in Saccharomyces cerevisiae","volume":"280","author":"Johnson","year":"2005","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib29","doi-asserted-by":"crossref","first-page":"3656","DOI":"10.1074\/jbc.M116.765222","article-title":"Polymorphic variants of human protein L-isoaspartyl methyltransferase affect catalytic activity, aggregation, and thermal stability: implications for the etiology of neurological disorders and cognitive aging","volume":"292","author":"Juang","year":"2017","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib30","doi-asserted-by":"crossref","first-page":"S83","DOI":"10.1080\/13693780600835773","article-title":"LaeA, a global regulator of Aspergillus toxins","volume":"44","author":"Keller","year":"2006","journal-title":"Med Mycol"},{"key":"2026041420155530600_bib31","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat Protoc"},{"key":"2026041420155530600_bib32","doi-asserted-by":"crossref","first-page":"W526","DOI":"10.1093\/nar\/gkh468","article-title":"Protein structure prediction and analysis using the Robetta server","volume":"32","author":"Kim","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"2026041420155530600_bib33","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.ajhg.2007.12.024","article-title":"ADCK3, an ancestral kinase, is mutated in a form of recessive ataxia associated with coenzyme Q10 deficiency","volume":"82","author":"Lagier-Tourenne","year":"2008","journal-title":"The American Journal of Human Genetics"},{"key":"2026041420155530600_bib34","doi-asserted-by":"crossref","first-page":"D1062","DOI":"10.1093\/nar\/gkx1153","article-title":"ClinVar: improving access to variant interpretations and supporting evidence","volume":"46","author":"Landrum","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"2026041420155530600_bib35","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK - a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J Appl Crystallogr"},{"key":"2026041420155530600_bib36","doi-asserted-by":"crossref","first-page":"W256","DOI":"10.1093\/nar\/gkz239","article-title":"Interactive Tree Of Life (iTOL) v4: recent updates and new developments","volume":"47","author":"Letunic","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2026041420155530600_bib37","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1039\/c2np20029e","article-title":"Architectures, mechanisms and molecular evolution of natural product methyltransferases","volume":"29","author":"Liscombe","year":"2012","journal-title":"Nat Prod Rep"},{"key":"2026041420155530600_bib38","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.molcel.2018.11.033","article-title":"An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q Biosynthesis","volume":"73","author":"Lohman","year":"2019","journal-title":"Mol Cell"},{"key":"2026041420155530600_bib39","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/356083a0","article-title":"Assessment of protein models with three-dimensional profiles","volume":"356","author":"Luthy","year":"1992","journal-title":"Nature"},{"key":"2026041420155530600_bib40","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.bbalip.2008.10.006","article-title":"The yeast Coq4 polypeptide organizes a mitochondrial protein complex essential for coenzyme Q biosynthesis","volume":"1791","author":"Marbois","year":"2009","journal-title":"Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids"},{"key":"2026041420155530600_bib41","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0378-1119(94)90810-9","article-title":"Cloning of a rat cDNA encoding dihydroxypolyprenylbenzoate methyltransferase by functional complementation of a Saccharomyces cerevisiae mutant deficient in ubiquinone biosynthesis","volume":"138","author":"Marbois","year":"1994","journal-title":"Gene"},{"key":"2026041420155530600_bib42","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/S0959-440X(02)00391-3","article-title":"SAM (dependent) I AM: the S-adenosylmethionine-dependent methyltransferase fold","volume":"12","author":"Martin","year":"2002","journal-title":"Curr Opin Struct Biol"},{"key":"2026041420155530600_bib43","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1091\/mbc.e12-10-0749","article-title":"Modular assembly of yeast cytochrome oxidase","volume":"24","author":"McStay","year":"2013","journal-title":"Mol Biol Cell"},{"key":"2026041420155530600_bib44","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1056\/NEJMc0800582","article-title":"Early coenzyme Q10 supplementation in primary coenzyme Q10 deficiency","volume":"358","author":"Montini","year":"2008","journal-title":"N Engl J Med"},{"key":"2026041420155530600_bib45","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/yea.320080202","article-title":"A rapid method for localized mutagenesis of yeast genes","volume":"8","author":"Muhlrad","year":"1992","journal-title":"Yeast"},{"key":"2026041420155530600_bib46","doi-asserted-by":"crossref","first-page":"21665","DOI":"10.1074\/jbc.274.31.21665","article-title":"Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis","volume":"274","author":"Poon","year":"1999","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib47","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.biochi.2004.09.017","article-title":"Fatty acid biosynthesis in microorganisms being used for Single Cell Oil production","volume":"86","author":"Ratledge","year":"2004","journal-title":"Biochimie"},{"key":"2026041420155530600_bib48","doi-asserted-by":"crossref","first-page":"4417","DOI":"10.1074\/jbc.M113.515197","article-title":"A functional, genome-wide evaluation of liposensitive yeast identifies the \u201cARE2 required for viability\u201d (ARV1) gene product as a major component of eukaryotic fatty acid resistance","volume":"289","author":"Ruggles","year":"2014","journal-title":"J Biol Chem"},{"key":"2026041420155530600_bib49","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol Biol Evol"},{"key":"2026041420155530600_bib77_1637691185404","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.fuel.2012.12.014","article-title":"Biodiesel production from a newly isolated Pichia kudriavzevii strain","volume":"106","author":"Sankh","year":"2013","journal-title":"Fuel"},{"key":"2026041420155530600_bib76_1637690796867","doi-asserted-by":"publisher","first-page":"90547","DOI":"10.1039\/C6RA16382C","article-title":"Lipid production by Yarrowia lipolyticagrown on biodiesel-derived crude glycerol: optimization of growthparameters and their effects on the fermentation efficiency","volume":"6","author":"Sara","year":"2016","journal-title":"RSC Adv"},{"key":"2026041420155530600_bib50","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S0968-0004(03)00090-2","article-title":"Many paths to methyltransfer: a chronicle of convergence","volume":"28","author":"Schubert","year":"2003","journal-title":"Trends Biochem Sci"},{"key":"2026041420155530600_bib51","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.7150\/jca.30699","article-title":"Identification of key genes and pathways associated with esophageal squamous cell carcinoma development based on weighted gene correlation network analysis","volume":"11","author":"Shao","year":"2020","journal-title":"J Cancer"},{"key":"2026041420155530600_bib52","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol Syst Biol"},{"key":"2026041420155530600_bib53","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.str.2012.01.024","article-title":"Detection of spatial correlations in protein structures and molecular complexes","volume":"20","author":"Sippl","year":"2012","journal-title":"Structure"},{"key":"2026041420155530600_bib54","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1038\/nbt.3683","article-title":"Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling","volume":"34","author":"Stefely","year":"2016","journal-title":"Nat Biotechnol"},{"key":"2026041420155530600_bib55","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.tibs.2017.06.008","article-title":"Biochemistry of Mitochondrial Coenzyme Q Biosynthesis","volume":"42","author":"Stefely","year":"2017","journal-title":"Trends Biochem Sci"},{"key":"2026041420155530600_bib56","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1083\/jcb.201808044","article-title":"Coenzyme Q biosynthetic proteins assemble in a substrate-dependent manner into domains at ER-mitochondria contacts","volume":"218","author":"Subramanian","year":"2019","journal-title":"J Cell Biol"},{"key":"2026041420155530600_bib57","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.apsb.2020.08.011","article-title":"Diversity of the reaction mechanisms of SAM-dependent enzymes","volume":"11","author":"Sun","year":"2021","journal-title":"Acta Pharmaceutica Sinica B"},{"key":"2026041420155530600_bib58","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.cmet.2020.03.011","article-title":"Systems biochemistry approaches to defining mitochondrial protein function","volume":"31","author":"Sung","year":"2020","journal-title":"Cell Metab"},{"key":"2026041420155530600_bib59","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1111\/j.1567-1364.2008.00436.x","article-title":"Ubiquinone biosynthesis in Saccharomyces cerevisiae: the molecular organization of O-methylase Coq3p depends on Abc1p\/Coq8p","volume":"8","author":"Tauche","year":"2008","journal-title":"FEMS Yeast Res"},{"key":"2026041420155530600_bib60","doi-asserted-by":"crossref","first-page":"D506","DOI":"10.1093\/nar\/gky1049","article-title":"UniProt: a worldwide hub of protein knowledge","volume":"47","author":"The_Uniprot_Consortium","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2026041420155530600_bib61","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/0092-8674(89)90584-9","article-title":"Elevated recombination rates in transcriptionally active DNA","volume":"56","author":"Thomas","year":"1989","journal-title":"Cell"},{"key":"2026041420155530600_bib62","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.bbadis.2016.10.005","article-title":"Leucine 208 in human histamine N-methyltransferase emerges as a hotspot for protein stability rationalizing the role of the L208P variant in intellectual disability","volume":"1863","author":"Tongsook","year":"2017","journal-title":"Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease"},{"issue":"Suppl","key":"2026041420155530600_bib63","doi-asserted-by":"crossref","first-page":"S62","DOI":"10.1016\/j.mito.2007.03.007","article-title":"Endogenous synthesis of coenzyme Q in eukaryotes","volume":"7","author":"Tran","year":"2007","journal-title":"Mitochondrion"},{"key":"2026041420155530600_bib64","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1128\/mr.54.3.211-225.1990","article-title":"PET genes of Saccharomyces cerevisiae","volume":"54","author":"Tzagoloff","year":"1990","journal-title":"Microbiol Rev"},{"key":"2026041420155530600_bib65","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1007\/s00253-019-09742-x","article-title":"Oleaginous yeasts for sustainable lipid production-from biodiesel to surf boards, a wide range of \u201cgreen\u201d applications","volume":"103","author":"Vasconcelos","year":"2019","journal-title":"Appl Microbiol Biotechnol"},{"key":"2026041420155530600_bib66","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1038\/s41467-019-12438-5","article-title":"Landscape of multi-nucleotide variants in 125,748 human exomes and 15,708 genomes","volume":"11","author":"Wang","year":"2020","journal-title":"Nat Commun"},{"key":"2026041420155530600_bib75_1637690042202","doi-asserted-by":"crossref","first-page":"7027","DOI":"10.15376\/biores.9.4.7027-7040","article-title":"Oil production by theoleaginous yeast Lipomyces starkeyi using diverse carbon sources","volume":"9","author":"Wang","year":"2014","journal-title":"BioResources"},{"key":"2026041420155530600_bib67","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","article-title":"Jalview Version 2\u2013a multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"2026041420155530600_bib68","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.str.2014.05.005","article-title":"Structure-based characterization of multiprotein complexes","volume":"22","author":"Wiederstein","year":"2014","journal-title":"Structure"},{"key":"2026041420155530600_bib69","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.bbalip.2011.01.009","article-title":"Expression of the human atypical kinase ADCK3 rescues coenzyme Q biosynthesis and phosphorylation of Coq polypeptides in yeast coq8 mutants","volume":"1811","author":"Xie","year":"2011","journal-title":"Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids"},{"key":"2026041420155530600_doi73_979_232021","first-page":"86","article-title":"Recycling biodiesel-derived glycerol by the oleaginous yeast\u00a0Rhodosporidium toruloides\u00a0Y4 through the two-stage lipid production process","volume-title":"Biochemical Engineering Journal","author":"Yang","year":"2014"},{"key":"2026041420155530600_bib70","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.bbrc.2010.09.118","article-title":"Over-expression of COQ10 in Saccharomyces cerevisiae inhibits mitochondrial respiration","volume":"402","author":"Zampol","year":"2010","journal-title":"Biochem Biophys Res Commun"},{"key":"2026041420155530600_doi74_509_232721","first-page":"424","article-title":"Lipid Production for Biodiesel from Sludge and Crude Glycerol","volume-title":"Water Environment Research","author":"Zhang","year":"2017"},{"key":"2026041420155530600_bib71","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2105-9-40","article-title":"I-TASSER server for protein 3D structure prediction","volume":"9","author":"Zhang","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"2026041420155530600_bib72","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1042\/BJ20150329","article-title":"Structural and biochemical studies reveal UbiG\/Coq3 as a class of novel membrane-binding proteins","volume":"470","author":"Zhu","year":"2015","journal-title":"Biochem J"}],"container-title":["FEMS Yeast Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/femsyr\/advance-article-pdf\/doi\/10.1093\/femsyr\/foab055\/41121522\/foab055.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsyr\/article-pdf\/21\/7\/foab055\/41641384\/foab055.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsyr\/article-pdf\/21\/7\/foab055\/41641384\/foab055.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:28:30Z","timestamp":1776212910000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsyr\/article\/doi\/10.1093\/femsyr\/foab055\/6424906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":77,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,12,7]]}},"URL":"https:\/\/doi.org\/10.1093\/femsyr\/foab055","relation":{},"ISSN":["1567-1364"],"issn-type":[{"value":"1567-1364","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,11]]},"published":{"date-parts":[[2021,11]]},"article-number":"foab055"}}