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To explore the function of CgErg6p in the yeast pathogen Candida glabrata, we have constructed the Cgerg6\u0394 deletion mutant. We found that C. glabrata cells lacking CgErg6p exhibit reduced susceptibility to both antifungal azoles and polyenes. The reduced content of ergosterol in the Cgerg6 deletion mutant was accompanied by increased expression of genes encoding the last steps of the ergosterol biosynthetic pathway. The absence of CgErg6p leads to plasma membrane hyperpolarization and decrease in its fluidity compared to the parental C. glabrata strain. The absence of sterols containing C-24 alkyls influenced the susceptibility of Cgerg6\u0394 mutant cells to alkali metal cations and several other metabolic inhibitors. Our results thus show that sterols lacking C-24 alkyls are not sufficient substitutes for maintaining yeast plasma membrane function. The absence of CgErg6p influences also the cell wall integrity and calcineurin signaling in C. glabrata.<\/jats:p>","DOI":"10.1093\/femsyr\/foac045","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T14:56:24Z","timestamp":1662044184000},"source":"Crossref","is-referenced-by-count":22,"title":["Erg6p is essential for antifungal drug resistance, plasma membrane properties and cell wall integrity in <i>Candida glabrata<\/i>"],"prefix":"10.1093","volume":"22","author":[{"given":"Daniel","family":"Elias","sequence":"first","affiliation":[{"name":"Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava , Ilkovicova 6, 842 15 Bratislava, SlovkRepublic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2784-1032","authenticated-orcid":false,"given":"Nora","family":"Toth\u00a0Hervay","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava , Ilkovicova 6, 842 15 Bratislava, SlovkRepublic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juraj","family":"Jacko","sequence":"additional","affiliation":[{"name":"Faculty of Mathematics, Physics and Informatics, Department of Nuclear Physics and Biophysics, Comenius University in Bratislava , Mlynska dolina, 842 48 Bratislava, SlovkRepublic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Marcela","family":"Morvova","sequence":"additional","affiliation":[{"name":"Faculty of Mathematics, Physics and Informatics, Department of Nuclear Physics and Biophysics, Comenius University in Bratislava , Mlynska dolina, 842 48 Bratislava, SlovkRepublic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Valachovic","sequence":"additional","affiliation":[{"name":"Biochemistry of Biomembranes, Institute of Animal Biochemistry and Genetics CBS SAS , Dubravska cesta 9, 840 05 Bratislava, SlovkRepublic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7516-8463","authenticated-orcid":false,"given":"Yvetta","family":"Gbelska","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Department of Microbiology and Virology, Comenius University in Bratislava , Ilkovicova 6, 842 15 Bratislava, SlovkRepublic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,9,1]]},"reference":[{"key":"2022111112295337200_bib1","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.bbamem.2008.12.002","article-title":"Mechanistic role of ergosterol in membrane rigidity and cycloheximide resistance in saccharomycescerevisiae","volume":"1788","author":"Abe","year":"2009","journal-title":"Biochimica Et Biophysica Acta (BBA) - 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