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While hypoxia is the prevalent condition within the host, the machinery that integrates oxygen status to tune the fitness of fungal pathogens remains poorly characterized. Here, we uncovered that Snf5, a subunit of the chromatin remodeling complex SWI\/SNF, is required to tolerate antifungal stress particularly under hypoxia. RNA-seq profiling of snf5 mutant exposed to amphotericin B and fluconazole under hypoxic conditions uncovered a signature that is reminiscent of copper (Cu) starvation. We found that under hypoxic and Cu-starved environments, Snf5 is critical for preserving Cu homeostasis and the transcriptional modulation of the Cu regulon. Furthermore, snf5 exhibits elevated levels of reactive oxygen species and an increased sensitivity to oxidative stress principally under hypoxia. Supplementing growth medium with Cu or increasing gene dosage of the Cu transporter CTR1 alleviated snf5 growth defect and attenuated reactive oxygen species levels in response to antifungal challenge. Genetic interaction analysis suggests that Snf5 and the bona fide Cu homeostasis regulator Mac1 function in separate pathways. Together, our data underlined a unique role of SWI\/SNF complex as a potent regulator of Cu metabolism and antifungal stress under hypoxia.<\/jats:p>","DOI":"10.1093\/femsyr\/foae018","type":"journal-article","created":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T03:54:14Z","timestamp":1716004454000},"source":"Crossref","is-referenced-by-count":6,"title":["Regulation of copper uptake by the SWI\/SNF chromatin remodeling complex in <i>Candida albicans<\/i> affects susceptibility to antifungal and oxidative stresses under hypoxia"],"prefix":"10.1093","volume":"24","author":[{"given":"In\u00e8s","family":"Khemiri","sequence":"first","affiliation":[{"name":"Montreal Heart Institute\/Institut de Cardiologie de Montr\u00e9al, Universit\u00e9 de Montr\u00e9al , 5000 Rue B\u00e9langer, Montr\u00e9al, QC H1T 1C8 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faiza","family":"Tebbji","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute\/Institut de Cardiologie de Montr\u00e9al, Universit\u00e9 de Montr\u00e9al , 5000 Rue B\u00e9langer, Montr\u00e9al, QC H1T 1C8 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana\u00efs","family":"Burgain","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval , Quebec City, QC ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0506-7752","authenticated-orcid":false,"given":"Adnane","family":"Sellam","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute\/Institut de Cardiologie de Montr\u00e9al, Universit\u00e9 de Montr\u00e9al , 5000 Rue B\u00e9langer, Montr\u00e9al, QC H1T 1C8 ,","place":["Canada"]},{"name":"Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 de Montr\u00e9al , Montr\u00e9al, QC 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