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The aim of this study was to reveal markers for rapidly identifying the predominant azole-resistant C. tropicalis genotype. We analysed XYR1, one of the six genes used in the multilocus sequence typing analysis, and SNQ2, an ATP-binding cassette transporter in 281 C. tropicalis, including 120 and 161 from Taiwan and global areas, respectively. Intriguingly, the first 4-mer of codon sequences ATRA of CTRG_05978 (96\/119\u00a0versus 21\/162, P\u00a0&amp;lt;\u00a0.001, at phi\u00a0=\u00a00. 679) and the SNQ2 A2977G resulting in amino acid I993V alternation (105\/118\u00a0versus 12\/163, P\u00a0&amp;lt;\u00a0.001, at phi\u00a0=\u00a00.81) was significantly associated with the clade 4 genotype. The sensitivity and specificity of the clade 4 genotype detection with a combination of SNPs of CTRG_05978 and SNQ2 were 0.812 and 0.994, respectively, at phi\u00a0=\u00a00.838. Furthermore, we successfully established a TaqMan SNP genotyping assay to identify the clade 4 genotype. Our findings suggest that to improve the management of C. tropicalis infections, rapidly identifying azole-resistant C. tropicalis by detecting SNPs of CTRG_05978 and SNQ2 is promising.<\/jats:p>","DOI":"10.1093\/femsyr\/foae025","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T09:56:06Z","timestamp":1729590966000},"source":"Crossref","is-referenced-by-count":5,"title":["Rapid identification of the predominant azole-resistant genotype in <i>Candida tropicalis<\/i>"],"prefix":"10.1093","volume":"24","author":[{"given":"Kuo-Yun","family":"Tseng","sequence":"first","affiliation":[{"name":"Taiwan Mycology Reference Center, Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 350401 ,","place":["Taiwan, ROC"]},{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University , Hsinchu 300044 ,","place":["Taiwan, 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Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 350401 ,","place":["Taiwan, ROC"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey-Kang","family":"Sytwu","sequence":"additional","affiliation":[{"name":"Taiwan Mycology Reference Center, Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 350401 ,","place":["Taiwan, ROC"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Yun","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Taiwan Mycology Reference Center, Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 350401 ,","place":["Taiwan, ROC"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Yu","family":"Lan","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University , Hsinchu 300044 ,","place":["Taiwan, ROC"]},{"name":"Department of Life Science, National Tsing Hua University , Hsinchu 300044 ,","place":["Taiwan, ROC"]},{"name":"School of Medicine, National Tsing Hua University , Hsinchu 300044 ,","place":["Taiwan, ROC"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-5214","authenticated-orcid":false,"given":"Hsiu-Jung","family":"Lo","sequence":"additional","affiliation":[{"name":"Taiwan Mycology Reference Center, Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Miaoli 350401 ,","place":["Taiwan, ROC"]},{"name":"School of Dentistry, China Medical University , Taichung 404328 ,","place":["Taiwan, ROC"]},{"name":"Department of Biological Science and Technology, National Yang Ming Chiao Tung University , Hsinchu 300093 ,","place":["Taiwan, 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