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Strains of 52 Saccharomycotina yeasts were screened for growth on ethanol. The 21 fastest strains, among which representatives of the Phaffomycetales order were overrepresented, showed specific growth rates in ethanol-grown shake-flask cultures between 0.12 and 0.46 h\u22121. Seven strains were studied in aerobic, ethanol-limited chemostats (dilution rate 0.10 h\u22121). Saccharomyces cerevisiae and Kluyveromyces lactis, whose genomes do not encode Complex-I-type NADH dehydrogenases, showed biomass yields of 0.59 and 0.56 gbiomass gethanol\u22121, respectively. Different biomass yields were observed among species whose genomes do harbour Complex-I-encoding genes: Phaffomyces thermotolerans (0.58 g g\u22121), Pichia ethanolica (0.59 g g\u22121), Saturnispora dispora (0.66 g g\u22121), Ogataea parapolymorpha (0.67 g g\u22121), and Cyberlindnera jadinii (0.73 g g\u22121). Cyberlindnera jadinii biomass showed the highest protein content (59\u00a0\u00b1\u00a02%) of these yeasts. Its biomass yield corresponded to 88% of the theoretical maximum that is reached when growth is limited by assimilation rather than by energy availability. This study suggests that energy coupling of mitochondrial respiration and its regulation will become key factors for selecting and improving yeast strains for ethanol-based processes.<\/jats:p>","DOI":"10.1093\/femsyr\/foae037","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T17:57:21Z","timestamp":1733853441000},"source":"Crossref","is-referenced-by-count":8,"title":["Specific growth rates and growth stoichiometries of Saccharomycotina yeasts on ethanol as sole carbon and energy substrate"],"prefix":"10.1093","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4927-6432","authenticated-orcid":false,"given":"Marieke","family":"Warmerdam","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Delft University of Technology , Van der Maasweg 9, 2629 HZ Delft,","place":["the 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