{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:18:27Z","timestamp":1789024707890,"version":"build-2803163510"},"reference-count":143,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T00:00:00Z","timestamp":1742688000000},"content-version":"vor","delay-in-days":81,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFC3407100"],"award-info":[{"award-number":["2024YFC3407100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["ZR2022ZD24"],"award-info":[{"award-number":["ZR2022ZD24"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011374","name":"SKLMT","doi-asserted-by":"publisher","award":["SKLMTFCP-2023"],"award-info":[{"award-number":["SKLMTFCP-2023"]}],"id":[{"id":"10.13039\/501100011374","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011374","name":"SKLMT","doi-asserted-by":"publisher","award":["03"],"award-info":[{"award-number":["03"]}],"id":[{"id":"10.13039\/501100011374","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Saccharomyces cerevisiae is a promising microbial cell factory. However, the overflow metabolism, known as the Crabtree effect, directs the majority of the carbon source toward ethanol production, in many cases, resulting in low yields of other target chemicals and byproducts accumulation. To construct Crabtree-negative S. cerevisiae, the deletion of pyruvate decarboxylases and\/or ethanol dehydrogenases is required. However, these modifications compromises the growth of the strains on glucose. This review discusses the metabolic engineering approaches used to eliminate ethanol production, the efforts to alleviate growth defect of Crabtree-negative strains, and the underlying mechanisms of the growth rescue. In addition, it summarizes the applications of Crabtree-negative S. cerevisiae in the synthesis of various chemicals such as lactic acid, 2,3-butanediol, malic acid, succinic acid, isobutanol, and others.<\/jats:p>","DOI":"10.1093\/femsyr\/foaf014","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T06:44:51Z","timestamp":1742712291000},"source":"Crossref","is-referenced-by-count":28,"title":["The advances in creating Crabtree-negative\n                    <i>Saccharomyces cerevisiae<\/i>\n                    and the application for chemicals biosynthesis"],"prefix":"10.1093","volume":"25","author":[{"given":"Yalin","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University , Binhai Road 72 , Qingdao, Shandong, 266237,","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Xiong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University , Binhai Road 72 , Qingdao, Shandong, 266237,","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Zhai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University , Binhai Road 72 , Qingdao, Shandong, 266237,","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University , Binhai Road 72 , Qingdao, Shandong, 266237,","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9015-4561","authenticated-orcid":false,"given":"Qingsheng","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University , Binhai Road 72 , Qingdao, Shandong, 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