{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:27:37Z","timestamp":1789705657278,"version":"4.0.1"},"reference-count":87,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":107,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007945","name":"SFG","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007945","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011035","name":"Standortagentur Tirol","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011035","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004955","name":"Austrian Research Promotion Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["10.55776\/W1224"],"award-info":[{"award-number":["10.55776\/W1224"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["10.55776\/I6795"],"award-info":[{"award-number":["10.55776\/I6795"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["10.55776\/I6812"],"award-info":[{"award-number":["10.55776\/I6812"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["10.55776\/COE17"],"award-info":[{"award-number":["10.55776\/COE17"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["101000441"],"award-info":[{"award-number":["101000441"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Single carbon (C1) molecules are considered as valuable substrates for biotechnology, as they serve as intermediates of carbon dioxide recycling, and enable bio-based production of a plethora of substances of our daily use without relying on agricultural plant production. Yeasts are valuable chassis organisms for biotech production, and they are able to use C1 substrates either natively or as synthetic engineered strains. This minireview highlights native yeast pathways for methanol and formate assimilation, their engineering, and the realization of heterologous C1 pathways including CO2, in different yeast species. Key features determining the choice among C1 substrates are discussed, including their chemical nature and specifics of their assimilation, their availability, purity, and concentration as raw materials, as well as features of the products to be made from them.<\/jats:p>","DOI":"10.1093\/femsyr\/foaf020","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T02:28:22Z","timestamp":1744943302000},"source":"Crossref","is-referenced-by-count":14,"title":["Advancing yeast metabolism for a sustainable single carbon bioeconomy"],"prefix":"10.1093","volume":"25","author":[{"given":"Miriam","family":"Kuzman","sequence":"first","affiliation":[{"name":"BOKU University Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, , 1190, Vienna ,","place":["Austria"]},{"name":"Austrian Centre of Industrial Biotechnology (ACIB) , 1190, Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7157-2039","authenticated-orcid":false,"given":"\u00d6zge","family":"Ata","sequence":"additional","affiliation":[{"name":"BOKU University Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, , 1190, Vienna ,","place":["Austria"]},{"name":"Austrian Centre of Industrial Biotechnology (ACIB) , 1190, Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0907-4167","authenticated-orcid":false,"given":"Diethard","family":"Mattanovich","sequence":"additional","affiliation":[{"name":"BOKU University Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science, , 1190, Vienna ,","place":["Austria"]},{"name":"Austrian Centre of Industrial Biotechnology (ACIB) , 1190, Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"2025042410362822800_bib1","first-page":"E9271","article-title":"Assimilation of formic acid and CO2 by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways","volume":"115 (40)","author":"Bang","year":"2018","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib2","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1007\/978-3-642-94798-8_30","article-title":"The path of carbon in photosynthesis","volume-title":"Die CO2-Assimilation\/the Assimilation of Carbon Dioxide. Handbuch der Pflanzenphysiologie \/ Encyclopedia of Plant Physiology","author":"Bassham","year":"1960"},{"key":"2025042410362822800_bib3","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.synbio.2024.03.003","article-title":"Single carbon metabolism\u2014a new paradigm for microbial bioprocesses?","volume":"9","author":"Baumschabl","year":"2024","journal-title":"Synth Syst Biotechnol"},{"key":"2025042410362822800_bib4","doi-asserted-by":"crossref","first-page":"e2211827119","DOI":"10.1073\/pnas.2211827119","article-title":"Conversion of CO2 into organic acids by engineered autotrophic yeast","volume":"119","author":"Baumschabl","year":"2022","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib5","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1126\/science.1149976","article-title":"A 3-hydroxypropionate\/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea","volume":"318","author":"Berg","year":"2007","journal-title":"Science"},{"key":"2025042410362822800_bib6","doi-asserted-by":"crossref","first-page":"5702","DOI":"10.1021\/acs.chemrev.2c00581","article-title":"Enzymatic conversion of CO2: from natural to artificial utilization","volume":"123","author":"Bierbaumer","year":"2023","journal-title":"Chem Rev"},{"key":"2025042410362822800_bib7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3389\/fenrg.2019.00081","article-title":"Methanol production via CO2 hydrogenation: sensitivity analysis and simulation-based optimization","volume":"7","author":"Borisut","year":"2019","journal-title":"Front Energy Res"},{"key":"2025042410362822800_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.ymben.2023.02.004","article-title":"Paving the way for synthetic C1-metabolism in Pseudomonas putida through the reductive glycine pathway","volume":"76","author":"Bruinsma","year":"2023","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib9","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.ymben.2023.11.007","article-title":"Engineering and evolution of the complete reductive glycine pathway in Saccharomyces cerevisiae for formate and CO2 assimilation","volume":"81","author":"Bysani","year":"2024","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib10","doi-asserted-by":"crossref","first-page":"7791","DOI":"10.1093\/nar\/gkab535","article-title":"Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris","volume":"49","author":"Cai","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2025042410362822800_bib11","doi-asserted-by":"crossref","first-page":"e2201711119","DOI":"10.1073\/pnas.2201711119","article-title":"Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris","volume":"119","author":"Cai","year":"2022","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib12","doi-asserted-by":"crossref","first-page":"7512","DOI":"10.1021\/acscatal.0c01487","article-title":"In vivo selection for formate dehydrogenases with high efficiency and specificity toward NADP+","volume":"10","author":"Calzadiaz-Ramirez","year":"2020","journal-title":"ACS Catal"},{"key":"2025042410362822800_bib13","doi-asserted-by":"crossref","first-page":"2400290","DOI":"10.1002\/biot.202400290","article-title":"Unlocking the formate utilization of wild-type Yarrowia lipolytica through adaptive laboratory evolution","volume":"19","author":"Chen","year":"2024","journal-title":"Biotechnol J"},{"key":"2025042410362822800_bib14","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1146\/annurev.micro.091208.073600","article-title":"The expanding world of methylotrophic metabolism","volume":"63","author":"Chistoserdova","year":"2009","journal-title":"Annu Rev Microbiol"},{"key":"2025042410362822800_bib15","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.ymben.2023.06.015","article-title":"Engineering the biological conversion of formate into crotonate in Cupriavidus necator","volume":"79","author":"Collas","year":"2023","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib16","doi-asserted-by":"crossref","first-page":"5057","DOI":"10.1128\/JB.00228-08","article-title":"Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1","volume":"190","author":"Crowther","year":"2008","journal-title":"J Bacteriol"},{"key":"2025042410362822800_bib17","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/j.biortech.2017.05.100","article-title":"Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae.","volume":"245","author":"Dai","year":"2017","journal-title":"Bioresour Technol"},{"key":"2025042410362822800_bib18","doi-asserted-by":"crossref","first-page":"107774","DOI":"10.1016\/j.bioorg.2024.107774","article-title":"Synthetic biology of metabolic cycles for enhanced CO2 capture and sequestration","volume":"153","author":"Dowaidar","year":"2024","journal-title":"Bioorg Chem"},{"key":"2025042410362822800_bib19","doi-asserted-by":"publisher","first-page":"preprint: not peer reviewed","DOI":"10.1101\/717942","article-title":"Engineering and evolution of methanol assimilation in Saccharomyces cerevisiae.","author":"Espinosa","year":"2020","journal-title":"bioRxiv"},{"key":"2025042410362822800_bib20","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1073\/pnas.55.4.928","article-title":"A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium","volume":"55","author":"Evans","year":"1966","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib21","doi-asserted-by":"crossref","first-page":"123811","DOI":"10.1016\/j.seppur.2023.123811","article-title":"Electrochemical reduction of CO2 into formate\/formic acid: a review of cell design and operation","volume":"316","author":"Ewis","year":"2023","journal-title":"Sep Purif Technol"},{"key":"2025042410362822800_bib22","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.tibtech.2020.08.008","article-title":"Synthetic methylotrophy in yeasts: towards a circular bioeconomy","volume":"39","author":"Fabarius","year":"2021","journal-title":"Trends Biotechnol"},{"key":"2025042410362822800_bib23","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.synbio.2021.04.005","article-title":"Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products","volume":"6","author":"Gao","year":"2021","journal-title":"Synth Syst Biotechnol"},{"key":"2025042410362822800_bib24","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1038\/s42255-022-00601-0","article-title":"Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol","volume":"4","author":"Gao","year":"2022","journal-title":"Nat Metab"},{"key":"2025042410362822800_bib25","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1021\/jacsau.4c00106","article-title":"Engineering yeast peroxisomes for \u03b1-bisabolene production from sole methanol with the aid of proteomic analysis","volume":"4","author":"Gao","year":"2024","journal-title":"JACS Au"},{"key":"2025042410362822800_bib26","doi-asserted-by":"crossref","first-page":"2400261","DOI":"10.1002\/biot.202400261","article-title":"Engineering a versatile yeast platform for sesquiterpene production from glucose or methanol","volume":"19","author":"Gao","year":"2024","journal-title":"Biotechnol J"},{"key":"2025042410362822800_bib27","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2217\/fmb.12.133","article-title":"Pichia pastoris: protein production host and model organism for biomedical research","volume":"8","author":"Gasser","year":"2013","journal-title":"Future Microbiol"},{"key":"2025042410362822800_bib30","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/978-1-4939-9024-5_9","article-title":"CRISPR\/Cas9-mediated homology-directed genome editing in Pichia pastoris","volume-title":"Recombinant Protein Production in Yeast","author":"Gassler","year":"2019"},{"key":"2025042410362822800_bib28","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ymben.2021.11.007","article-title":"Adaptive laboratory evolution and reverse engineering enhances autotrophic growth in Pichia pastoris.","volume":"69","author":"Gassler","year":"2022","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib29","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41587-019-0363-0","article-title":"The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2","volume":"38","author":"Gassler","year":"2020","journal-title":"Nat Biotechnol"},{"key":"2025042410362822800_bib31","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s002530000464","article-title":"Heterologous protein production in methylotrophic yeasts","volume":"54","author":"Gellissen","year":"2000","journal-title":"Appl Microbiol Biotechnol"},{"key":"2025042410362822800_bib32","volume-title":"Fossil fuel CO 2 emissions increase again in 2024","author":"Global Carbon Budget","year":"2024"},{"key":"2025042410362822800_bib33","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1021\/acssynbio.8b00464","article-title":"Core catalysis of the reductive glycine pathway demonstrated in yeast","volume":"8","author":"Gonzalez\u00a0De\u00a0La\u00a0Cruz","year":"2019","journal-title":"ACS Synth Biol"},{"key":"2025042410362822800_bib34","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1039\/C9EE01673B","article-title":"Plant-to-planet analysis of CO2-based methanol processes","volume":"12","author":"Gonz\u00e1lez-Garay","year":"2019","journal-title":"Energy Environ Sci"},{"key":"2025042410362822800_bib35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1754-6834-6-125","article-title":"Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast","volume":"6","author":"Guadalupe-Medina","year":"2013","journal-title":"Biotechnol Biofuels"},{"key":"2025042410362822800_bib36","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/bit.27575","article-title":"Metabolic engineering of Pichia pastoris for malic acid production from methanol","volume":"118","author":"Guo","year":"2021","journal-title":"Biotechnol Bioeng"},{"key":"2025042410362822800_bib37","doi-asserted-by":"crossref","first-page":"eadq3484","DOI":"10.1126\/sciadv.adq3484","article-title":"Evolutionary engineering of Saccharomyces cerevisiae: crafting a synthetic methylotroph via self-reprogramming","volume":"10","author":"Guo","year":"2024","journal-title":"Sci Adv"},{"key":"2025042410362822800_bib38","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/j.tibtech.2023.03.006","article-title":"Bioconversion of C1 feedstocks for chemical production using Pichia pastoris.","volume":"41","author":"Guo","year":"2023","journal-title":"Trends Biotechnol"},{"key":"2025042410362822800_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymben.2024.05.002","article-title":"Engineering yeasts to Co-utilize methanol or formate coupled with CO2 fixation","volume":"84","author":"Guo","year":"2024","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib40","doi-asserted-by":"crossref","first-page":"e0247135","DOI":"10.1371\/journal.pone.0247135","article-title":"Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system","volume":"16","author":"Ha-Tran","year":"2021","journal-title":"PLoS One"},{"key":"2025042410362822800_bib41","doi-asserted-by":"crossref","first-page":"7851","DOI":"10.1073\/pnas.0801043105","article-title":"A dicarboxylate\/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis","volume":"105","author":"Huber","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib42","doi-asserted-by":"crossref","first-page":"142733","DOI":"10.1016\/j.electacta.2023.142733","article-title":"On the stability of electrochemical CO2 reduction reaction to formate at indium electrodes at biocompatible conditions","volume":"462","author":"Izadi","year":"2023","journal-title":"Electrochim Acta"},{"key":"2025042410362822800_bib43","doi-asserted-by":"crossref","first-page":"2000431","DOI":"10.1002\/biot.202000431","article-title":"L-malic acid production from xylose by engineered Saccharomyces cerevisiae","volume":"17","author":"Kang","year":"2022","journal-title":"Biotechnol J"},{"key":"2025042410362822800_bib44","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.4014\/jmb.1003.03004","article-title":"Fermentation process development of recombinant Hansenula polymorpha for gamma-linolenic acid production","volume":"20","author":"Khongto","year":"2010","journal-title":"J Microbiol Biotechnol"},{"key":"2025042410362822800_bib45","doi-asserted-by":"crossref","first-page":"1091899","DOI":"10.3389\/fbioe.2023.1091899","article-title":"Optimizing E. coli as a formatotrophic platform for bioproduction via the reductive glycine pathway","volume":"11","author":"Kim","year":"2023","journal-title":"Front Bioeng Biotechnol"},{"key":"2025042410362822800_bib46","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/s41589-020-0473-5","article-title":"Growth of E. coli on formate and methanol via the reductive glycine pathway","volume":"16","author":"Kim","year":"2020","journal-title":"Nat Chem Biol"},{"key":"2025042410362822800_bib47","article-title":"Trends in globally-averaged CO2 determined from NOAA Global Monitoring Laboratory measurements","author":"Lan","year":"2025"},{"key":"2025042410362822800_bib48","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.ymben.2024.08.006","article-title":"Engineering yeast for high-level production of \u03b2-farnesene from sole methanol","volume":"85","author":"Li","year":"2024","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib49","doi-asserted-by":"crossref","first-page":"43875","DOI":"10.1038\/srep43875","article-title":"Engineered yeast with a CO2-fixation pathway to improve the bio-ethanol production from xylose-mixed sugars","volume":"7","author":"Li","year":"2017","journal-title":"Sci Rep"},{"key":"2025042410362822800_bib50","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1146\/annurev.mi.40.100186.002215","article-title":"The autotrophic pathway of acetate synthesis in acetogenic bacteria","volume":"40","author":"Ljungdhal","year":"1986","journal-title":"Annu Rev Microbiol"},{"key":"2025042410362822800_bib51","doi-asserted-by":"crossref","first-page":"2400347","DOI":"10.1002\/biot.202400347","article-title":"Facilitating secretory expression of apple seed \u03b2-glucosidase in Komagataella phaffii for the efficient preparation of salidroside","volume":"19","author":"Lu","year":"2024","journal-title":"Biotechnol J"},{"key":"2025042410362822800_bib52","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/s41929-022-00746-x","article-title":"A cell-free self-replenishing CO2-fixing system","volume":"5","author":"Luo","year":"2022","journal-title":"Nat Catal"},{"key":"2025042410362822800_bib53","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1002\/yea.1208","article-title":"Heterologous protein production using the Pichia pastoris expression system","volume":"22","author":"Macauley-Patrick","year":"2005","journal-title":"Yeast"},{"key":"2025042410362822800_bib54","doi-asserted-by":"crossref","first-page":"7754","DOI":"10.1038\/s41467-023-43610-7","article-title":"The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO2in the yeast Komagataella phaffii","volume":"14","author":"Mitic","year":"2023","journal-title":"Nat Commun"},{"key":"2025042410362822800_bib55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-017-1001-z","article-title":"Optimizing anaerobic growth rate and fermentation kinetics in Saccharomyces cerevisiae strains expressing Calvin-cycle enzymes for improved ethanol yield","volume":"11","author":"Papapetridis","year":"2018","journal-title":"Biotechnol Biofuels"},{"key":"2025042410362822800_bib56","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ymben.2018.04.017","article-title":"Metabolic engineering of Pichia pastoris.","volume":"50","author":"Pe\u00f1a","year":"2018","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib57","doi-asserted-by":"crossref","first-page":"131913","DOI":"10.1016\/j.biortech.2024.131913","article-title":"Advanced aspects of acetogens","volume":"427","author":"Poehlein","year":"2024","journal-title":"Bioresour Technol"},{"key":"2025042410362822800_bib58","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s12918-017-0492-3","article-title":"GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris","volume":"11","author":"Prielhofer","year":"2017","journal-title":"BMC Syst Biol"},{"key":"2025042410362822800_bib59","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s12915-015-0186-5","article-title":"Systems-level organization of yeast methylotrophic lifestyle","volume":"13","author":"Ru\u00dfmayer","year":"2015","journal-title":"BMC Biol"},{"key":"2025042410362822800_bib60","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.cogsc.2019.01.005","article-title":"Recent advances in industrial CO2 electroreduction","volume":"16","author":"S\u00e1nchez","year":"2019","journal-title":"Curr Opin Green Sustain Chem"},{"key":"2025042410362822800_bib61","doi-asserted-by":"crossref","first-page":"5090","DOI":"10.1038\/s41467-020-18906-7","article-title":"The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans","volume":"11","author":"S\u00e1nchez-Andrea","year":"2020","journal-title":"Nat Commun"},{"key":"2025042410362822800_bib62","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1126\/science.aah5237","article-title":"A synthetic pathway for the fixation of carbon dioxide in vitro","volume":"354","author":"Schwander","year":"2016","journal-title":"Science"},{"key":"2025042410362822800_bib63","doi-asserted-by":"crossref","first-page":"131396","DOI":"10.1016\/j.biortech.2024.131396","article-title":"Engineering high production of fatty alcohols from methanol by constructing coordinated dual biosynthetic pathways","volume":"412","author":"Shen","year":"2024","journal-title":"Bioresour Technol"},{"key":"2025042410362822800_bib64","doi-asserted-by":"crossref","first-page":"7516","DOI":"10.1073\/pnas.1912289117","article-title":"Functional cooperation of the glycine synthase-reductase and Wood\u2013Ljungdahl pathways for autotrophic growth of Clostridium drakei","volume":"117","author":"Song","year":"2020","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib65","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/978-3-642-79856-6_6","article-title":"Pichia pastoris","volume-title":"Nonconventional Yeasts in Biotechnology: A Handbook","author":"Sreekrishna","year":"1996"},{"key":"2025042410362822800_bib66","doi-asserted-by":"crossref","first-page":"7758","DOI":"10.1038\/s41467-023-43631-2","article-title":"Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery","volume":"14","author":"Tian","year":"2023","journal-title":"Nat Commun"},{"key":"2025042410362822800_bib67","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1007\/978-94-009-0213-8_2","article-title":"The ribulose monophosphate (Quayle) cycle: news and views","volume-title":"Microbial Growth on C1 Compounds: Proceedings of the 8th International Symposium on Microbial Growth on C1 Compounds, Held in San Diego, U.S.A., 27 August \u20131 September 1995","author":"Trotsenko","year":"1996"},{"key":"2025042410362822800_bib68","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ymben.2022.10.008","article-title":"Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation","volume":"74","author":"Turlin","year":"2022","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib69","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1002\/yea.3909","article-title":"Carbon efficient production of chemicals with yeasts","volume":"40","author":"V\u00e1squez\u00a0Castro","year":"2023","journal-title":"Yeast"},{"key":"2025042410362822800_bib70","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1021\/acssynbio.1c00464","article-title":"Engineering yeast Yarrowia lipolytica for methanol assimilation","volume":"10","author":"Wang","year":"2021","journal-title":"ACS Synth Biol"},{"key":"2025042410362822800_bib71","doi-asserted-by":"crossref","first-page":"108369","DOI":"10.1016\/j.bej.2022.108369","article-title":"Methanol oxidase from Hansenula polymorpha shows activity in peroxisome-deficient Pichia pastoris.","volume":"180","author":"Wang","year":"2022","journal-title":"Biochem Eng J"},{"key":"2025042410362822800_bib72","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1186\/s12934-024-02475-1","article-title":"Metabolic engineering of Komagataella phaffii for the efficient utilization of methanol","volume":"23","author":"Wang","year":"2024","journal-title":"Microb Cell Fact"},{"key":"2025042410362822800_bib73","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ymben.2024.10.007","article-title":"Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO2 via the Serine Threonine Cycle","volume":"88","author":"Wenk","year":"2025","journal-title":"Metab Eng"},{"key":"2025042410362822800_bib74","doi-asserted-by":"crossref","first-page":"860","DOI":"10.3390\/catal12080860","article-title":"Recent advances in heterogeneous electroreduction of CO2 on copper-based catalysts","volume":"12","author":"Wu","year":"2022","journal-title":"Catalysts"},{"key":"2025042410362822800_bib75","doi-asserted-by":"crossref","first-page":"2400115","DOI":"10.1002\/biot.202400115","article-title":"Efficient CRISPR-mediated C-to-T base editing in Komagataella phaffii","volume":"19","author":"Wu","year":"2024","journal-title":"Biotechnol J"},{"key":"2025042410362822800_bib76","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1021\/acssynbio.6b00167","article-title":"Recycling carbon dioxide during xylose fermentation by engineered Saccharomyces cerevisiae.","volume":"6","author":"Xia","year":"2017","journal-title":"ACS Synth Biol"},{"key":"2025042410362822800_bib77","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1021\/acscatal.1c04151","article-title":"A minimized synthetic carbon fixation cycle","volume":"12","author":"Xiao","year":"2022","journal-title":"ACS Catal"},{"key":"2025042410362822800_bib78","doi-asserted-by":"crossref","first-page":"e52086","DOI":"10.1371\/journal.pone.0052086","article-title":"Fumaric acid production in Saccharomyces cerevisiae by in silico aided metabolic engineering","volume":"7","author":"Xu","year":"2012","journal-title":"PLoS One"},{"key":"2025042410362822800_bib79","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.biotechadv.2017.11.002","article-title":"Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review","volume":"36","author":"Yang","year":"2018","journal-title":"Biotechnol Adv"},{"key":"2025042410362822800_bib80","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1021\/acssynbio.7b00086","article-title":"Engineered assimilation of exogenous and endogenous formate in Escherichia coli","volume":"6","author":"Yishai","year":"2017","journal-title":"ACS Synth Biol"},{"key":"2025042410362822800_bib81","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1038\/s41467-018-04417-z","article-title":"Augmenting the Calvin\u2013Benson\u2013Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway","volume":"9","author":"Yu","year":"2018","journal-title":"Nat Commun"},{"key":"2025042410362822800_bib82","doi-asserted-by":"crossref","first-page":"foab009","DOI":"10.1093\/femsyr\/foab009","article-title":"Beyond alcohol oxidase: the methylotrophic yeast Komagataella phaffii utilizes methanol also with its native alcohol dehydrogenase Adh2","volume":"21","author":"Zavec","year":"2021","journal-title":"FEMS Yeast Res"},{"key":"2025042410362822800_bib83","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1128\/AEM.02591-07","article-title":"Malic acid production by Saccharomyces cerevisiae: engineering of pyruvate carboxylation, oxaloacetate reduction, and malate export","volume":"74","author":"Zelle","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"2025042410362822800_bib84","doi-asserted-by":"crossref","first-page":"e2220816120","DOI":"10.1073\/pnas.2220816120","article-title":"Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast","volume":"120","author":"Zhai","year":"2023","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025042410362822800_bib85","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/s41929-023-00957-w","article-title":"Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph","volume":"6","author":"Zhan","year":"2023","journal-title":"Nat Catal"},{"key":"2025042410362822800_bib86","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1039\/D2GC02783F","article-title":"Improving methanol assimilation in Yarrowia lipolytica via systematic metabolic engineering combined with compartmentalization","volume":"25","author":"Zhang","year":"2023","journal-title":"Green Chem"},{"key":"2025042410362822800_bib87","doi-asserted-by":"crossref","first-page":"1800694","DOI":"10.1002\/biot.201800694","article-title":"Pichia pastoris as a versatile cell factory for the production of industrial enzymes and chemicals: current status and Future perspectives","volume":"14","author":"Zhu","year":"2019","journal-title":"Biotechnol J"}],"container-title":["FEMS Yeast Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/femsyr\/advance-article-pdf\/doi\/10.1093\/femsyr\/foaf020\/62951292\/foaf020.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsyr\/article-pdf\/doi\/10.1093\/femsyr\/foaf020\/62951292\/foaf020.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsyr\/article-pdf\/doi\/10.1093\/femsyr\/foaf020\/62951292\/foaf020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T14:36:48Z","timestamp":1745505408000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsyr\/article\/doi\/10.1093\/femsyr\/foaf020\/8115773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":87,"URL":"https:\/\/doi.org\/10.1093\/femsyr\/foaf020","relation":{},"ISSN":["1567-1364"],"issn-type":[{"value":"1567-1364","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025]]},"published":{"date-parts":[[2025]]},"article-number":"foaf020"}}