{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T02:24:57Z","timestamp":1777343097322,"version":"3.51.4"},"reference-count":50,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T00:00:00Z","timestamp":1746230400000},"content-version":"vor","delay-in-days":122,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Wolfson Family Chair in Biochemistry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Most laboratory strains of the yeast Saccharomyces cerevisiae are incapable of invading agar, to form large colonies (mats), and to develop filament-like structures (pseudohyphae). A prominent strain that manifests these morphologies is \u22111278b. While induced transcription of the FLO11 gene is critical for executing invasive growth, mat formation, and pseudohyphal growth, downregulation of the \u2018general stress response\u2019 also seems to be required. As this response is weak in \u22111278b cells, we assumed that they may be sensitives to stresses. We report, however, that they are resistant to various stressors, but severely sensitive specifically to NaCl. We found that this sensitivity is a result of mutations in the single \u22111278b\u2019s ENA gene, encoding P-type sodium ATPase. Other laboratory strains harbor three to five copies of ENA, suggesting that \u22111278b was selected against Ena activity. Obtaining \u22111278b cells that can grow on NaCl allows checking its effect on colony morphologies. In the presence of NaCl, \u22111278b\/ENA1+ cells do not invade agar, and do not form pseudohyphae or mats. Thus, we have found the following: (i) The \u22111278b strain differs from other laboratory strains with respect to sensitivity to NaCl, because it has no active Na+ ATPase exporter. (ii) NaCl is a suppressor of invasiveness, filamentous growth, and mat formation.<\/jats:p>","DOI":"10.1093\/femsyr\/foaf021","type":"journal-article","created":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T03:27:56Z","timestamp":1746242876000},"source":"Crossref","is-referenced-by-count":1,"title":["The <i>Saccharomyces cerevisiae<\/i> \u22111278b strain is sensitive to NaCl because of mutations in its <i>ENA1<\/i> gene"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6977-3285","authenticated-orcid":false,"given":"David","family":"Engelberg","sequence":"first","affiliation":[{"name":"The Institute of Life Science, The Hebrew University of Jerusalem Department of Biological Chemistry, , Jerusalem 91904 ,","place":["Israel"]},{"name":"Singapore\u2013HUJ Alliance for Research and Enterprise, Mechanisms of Liver Inflammatory Diseases Program, National University of Singapore , Singapore 138602"},{"name":"Yong Loo Lin School of Medicine, National 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