{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:01:06Z","timestamp":1753876866750,"version":"3.41.2"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:00:00Z","timestamp":1562716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100009193","name":"Royal Society of New Zealand Marsden Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009193","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Fungal infections are a major challenge to medicine and agriculture. Repeated and prophylactic use of antifungals can lead to pathogen cross-resistance to different classes of drugs. The early development of multidrug resistance in pathogenic fungi includes drug tolerance mediated by drug-dependent activation of drug efflux. In Saccharomyces cerevisiae and the fungal pathogen Candida glabrata, xenobiotic sensing motifs in transcription factors upregulate expression of several ATP-binding cassette (ABC) drug efflux pumps. We have therefore considered how drug candidates that trigger or prevent drug resistance could be identified and evaluated during drug discovery. We report a robust and sensitive, S. cerevisiae-based xenobiotic sensing system using the Pdr1 protein as a sensor and an attenuated version of the apoptotic murine BCL2-associated X (BAX) gene as a reporter. A molecular mechanism of attenuation that involves frameshift reversal may be associated with translation coupling and requires further investigation.<\/jats:p>","DOI":"10.1093\/femsyr\/foz048","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T16:25:57Z","timestamp":1562775957000},"source":"Crossref","is-referenced-by-count":0,"title":["Attenuated apoptotic BAX expression as a xenobiotic reporter in<i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1093","volume":"19","author":[{"given":"Mikhail V","family":"Keniya","sequence":"first","affiliation":[{"name":"The Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, PO Box 56, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian C","family":"Monk","sequence":"additional","affiliation":[{"name":"The Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, PO Box 56, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"key":"2021070602250613100_bib1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF02110333","article-title":"Yeast multidrug resistance: the PDR network","volume":"27","author":"Balzi","year":"1995","journal-title":"J Bioenerg Biomembr"},{"key":"2021070602250613100_bib2","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1099\/mic.0.2007\/010405-0","article-title":"Candida albicans drug resistance another way to cope with stress","volume":"153","author":"Cannon","year":"2007","journal-title":"Microbiology"},{"key":"2021070602250613100_bib3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1080\/03602530600569828","article-title":"Synthetic drugs and natural products as modulators of constitutive androstane receptor (CAR) and pregnane X receptor (PXR)","volume":"38","author":"Chang","year":"2006","journal-title":"Drug Metab Rev"},{"key":"2021070602250613100_bib4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10911-009-9109-9","article-title":"ABC transporters, drug resistance, and cancer stem cells","volume":"14","author":"Dean","year":"2009","journal-title":"J Mammary Gland Biol Neoplasia"},{"key":"2021070602250613100_bib5","doi-asserted-by":"crossref","first-page":"12612","DOI":"10.1074\/jbc.273.20.12612","article-title":"ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p","volume":"273","author":"Decottignies","year":"1998","journal-title":"J Biol Chem"},{"key":"2021070602250613100_bib6","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1124\/mol.107.038398","article-title":"Human pregnane X receptor antagonists and agonists define molecular requirements for different binding sites","volume":"72","author":"Ekins","year":"2007","journal-title":"Mol Pharmacol"},{"key":"2021070602250613100_bib7","doi-asserted-by":"crossref","first-page":"5063","DOI":"10.1074\/jbc.M610197200","article-title":"The central role of PDR1 in the foundation of yeast drug resistance","volume":"282","author":"Fardeau","year":"2007","journal-title":"J Biol Chem"},{"key":"2021070602250613100_bib8","first-page":"129","article-title":"Estrogens in unexpected places: possible implications for researchers and consumers","volume":"103","author":"Feldman","year":"1995","journal-title":"Environ Health Perspect"},{"key":"2021070602250613100_bib9","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1128\/MCB.20.9.3125-3136.2000","article-title":"Biochemical and genetic analysis of the mitochondrial response of yeast to BAX and BCL-X(L)","volume":"20","author":"Gross","year":"2000","journal-title":"Mol Cell Biol"},{"key":"2021070602250613100_bib10","doi-asserted-by":"crossref","first-page":"24439","DOI":"10.1016\/S0021-9258(18)54248-4","article-title":"Evidence for coupling between membrane and cytoplasmic domains of the yeast plasma membrane H(+)-ATPase. 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