{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T06:28:24Z","timestamp":1782973704480,"version":"3.54.5"},"reference-count":49,"publisher":"American Physiological Society","issue":"5","license":[{"start":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T00:00:00Z","timestamp":1629331200000},"content-version":"vor","delay-in-days":230,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01ES025229"],"award-info":[{"award-number":["R01ES025229"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["T32HL086350"],"award-info":[{"award-number":["T32HL086350"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["830629"],"award-info":[{"award-number":["830629"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cigarette smoke, including secondhand smoke (SHS), has significant detrimental vascular effects, but its effects on myogenic tone of small resistance arteries and the underlying mechanisms are understudied. Although it is apparent that SHS contributes to endothelial dysfunction, much less is known about how this toxicant alters arterial myocyte contraction, leading to alterations in myogenic tone. The study's goal is to determine the effects of SHS on mesenteric arterial myocyte contractility and excitability. C57BL\/6J\u00a0male mice were randomly assigned to either filtered air (FA) or SHS (6 h\/d, 5 d\/wk) exposed groups for a 4, 8, or 12-weeks period. Third and fourth-order mesenteric arteries and arterial myocytes were acutely isolated and evaluated with pressure myography and patch clamp electrophysiology, respectively. Myogenic tone was found to be elevated in mesenteric arteries from mice exposed to SHS for 12 wk but not for 4 or 8 wk. These results were correlated with an increase in L-type Ca2+ channel activity in mesenteric arterial myocytes after 12 wk of SHS exposure. Moreover, 12 wk SHS exposed arterial myocytes have reduced total potassium channel current density, which correlates with a depolarized membrane potential (Vm). These results suggest that SHS exposure induces alterations in key ionic conductances that modulate arterial myocyte contractility and myogenic tone. Thus, chronic exposure to an environmentally relevant concentration of SHS impairs mesenteric arterial myocyte electrophysiology and myogenic tone, which may contribute to increased blood pressure and risks of developing vascular complications due to passive exposure to cigarette smoke.<\/jats:p>","DOI":"10.1093\/function\/zqab041","type":"journal-article","created":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T08:41:48Z","timestamp":1629362508000},"source":"Crossref","is-referenced-by-count":13,"title":["Secondhand Smoke Exposure Impairs Ion Channel Function and Contractility of Mesenteric Arteries"],"prefix":"10.1152","volume":"2","author":[{"given":"Thanhmai","family":"Le","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Mart\u00edn-Arag\u00f3n\u00a0Baudel","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arsalan","family":"Syed","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navid","family":"Singhrao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyue","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor A","family":"Flores-Tamez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abby E","family":"Burns","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwun Nok Mimi","family":"Man","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Karey","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyoung","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes W","family":"Hell","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kent E","family":"Pinkerton","sequence":"additional","affiliation":[{"name":"Center for Health and the Environment, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Yin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1935-8400","authenticated-orcid":false,"given":"Madeline","family":"Nieves-Cintr\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of California Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","published-online":{"date-parts":[[2021,8,19]]},"reference":[{"issue":"5","key":"2021122118042518300_bib1","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1161\/01.CIR.92.5.1094","article-title":"Long-term cigarette smoking impairs endothelium-dependent coronary arterial vasodilator function","volume":"92","author":"Zeiher","year":"1995","journal-title":"Circulation"},{"issue":"16","key":"2021122118042518300_bib2","doi-asserted-by":"crossref","first-page":"e336","DOI":"10.1161\/CIR.0000000000000443","article-title":"Cardiovascular consequences of childhood secondhand tobacco smoke exposure: prevailing evidence, burden, and racial and socioeconomic disparities: a scientific statement from the American Heart Association","volume":"134","author":"Raghuveer","year":"2016","journal-title":"Circulation"},{"issue":"6","key":"2021122118042518300_bib3","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.amepre.2007.02.026","article-title":"The 2006 report of the surgeon general: the health consequences of involuntary exposure to tobacco smoke","volume":"32","author":"Moritsugu","year":"2007","journal-title":"Am J Prev Med"},{"issue":"2","key":"2021122118042518300_bib4","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.amepre.2018.08.013","article-title":"Healthcare costs of secondhand smoke exposure at home for U.S. children","volume":"56","author":"Yao","year":"2019","journal-title":"Am J Prev Med"},{"issue":"1","key":"2021122118042518300_bib5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ypmed.2017.12.028","article-title":"Healthcare costs attributable to secondhand smoke exposure at home for US adults","volume":"108","author":"Yao","year":"2018","journal-title":"Prev Med"},{"issue":"5","key":"2021122118042518300_bib6","doi-asserted-by":"crossref","first-page":"e013292","DOI":"10.1136\/bmjopen-2016-013292","article-title":"Modeling lifetime costs and health outcomes attributable to secondhand smoke exposure at home among Korean adult women","volume":"7","author":"Lee","year":"2017","journal-title":"BMJ Open"},{"issue":"7","key":"2021122118042518300_bib7","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1289\/ehp.1104413","article-title":"Exposure to secondhand smoke outside of a bar and a restaurant and tobacco exposure biomarkers in nonsmokers","volume":"120","author":"St\u00a0Helen","year":"2012","journal-title":"Environ Health Perspect"},{"issue":"1","key":"2021122118042518300_bib8","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s12971-015-0052-9","article-title":"The effects of Secondhand Smoke (SHS) exposure on microvascular endothelial function among healthy women","volume":"13","author":"Sanip","year":"2015","journal-title":"Tob Induc Dis"},{"issue":"1\u20133","key":"2021122118042518300_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejphar.2010.09.042","article-title":"Nitric oxide-mediated blood flow regulation as affected by smoking and nicotine","volume":"649","author":"Toda","year":"2010","journal-title":"Eur J Pharmacol"},{"issue":"3","key":"2021122118042518300_bib10","doi-asserted-by":"crossref","first-page":"H818","DOI":"10.1152\/ajpheart.00852.2011","article-title":"Secondhand tobacco smoke, arterial stiffness, and altered circadian blood pressure patterns are associated with lung inflammation and oxidative stress in rats","volume":"302","author":"Gentner","year":"2012","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"2","key":"2021122118042518300_bib11","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1002\/cphy.c160011","article-title":"Smooth muscle ion channels and regulation of vascular tone in resistance arteries and arterioles","volume":"7","author":"Tykocki","year":"2017","journal-title":"Compr Physiol"},{"issue":"2","key":"2021122118042518300_bib12","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1152\/physrev.1999.79.2.387","article-title":"Signaling mechanisms underlying the vascular myogenic response","volume":"79","author":"Davis","year":"1999","journal-title":"Physiol Rev"},{"issue":"1 Pt 2","key":"2021122118042518300_bib13","first-page":"H348","article-title":"Regulation of membrane potential and diameter by voltage-dependent K+ channels in rabbit myogenic cerebral arteries","volume":"269","author":"Knot","year":"1995","journal-title":"Am J Physiol"},{"issue":"1","key":"2021122118042518300_bib14","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1469-7793.1998.199br.x","article-title":"Regulation of arterial diameter and wall [Ca2+] in cerebral arteries of rat by membrane potential and intravascular pressure","volume":"508","author":"Knot","year":"1998","journal-title":"J Physiol"},{"issue":"3","key":"2021122118042518300_bib15","doi-asserted-by":"crossref","first-page":"H803","DOI":"10.1152\/ajpheart.00760.2010","article-title":"Fundamental increase in pressure-dependent constriction of brain parenchymal arterioles from subarachnoid hemorrhage model rats due to membrane depolarization","volume":"300","author":"Nystoriak","year":"2011","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"5","key":"2021122118042518300_bib16","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/S0735-1097(98)00217-4","article-title":"In-utero and neonatal exposure to secondhand smoke causes vascular dysfunction in newborn rats","volume":"32","author":"Hutchison","year":"1998","journal-title":"J Am Coll Cardiol"},{"issue":"2","key":"2021122118042518300_bib17","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.3892\/mmr.2015.3722","article-title":"Anti-oxidant effect of heme oxygenase-1 on cigarette smoke-induced vascular injury","volume":"12","author":"Yang","year":"2015","journal-title":"Mol Med Rep"},{"issue":"2","key":"2021122118042518300_bib18","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.atherosclerosis.2015.03.041","article-title":"Effect of smoking on endothelium-independent vasodilatation","volume":"240","author":"Lanza","year":"2015","journal-title":"Atherosclerosis"},{"issue":"6","key":"2021122118042518300_bib19","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1136\/tc.2009.033233","article-title":"A cross-sectional study on levels of secondhand smoke in restaurants and bars in five cities in China","volume":"20","author":"Liu","year":"2011","journal-title":"Tob Control"},{"issue":"13\u201315","key":"2021122118042518300_bib20","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1080\/15287394.2012.690690","article-title":"Occupational exposure to environmental tobacco smoke: a study in Lisbon restaurants","volume":"75","author":"Pacheco","year":"2012","journal-title":"J Toxicol Environ Health A"},{"issue":"7","key":"2021122118042518300_bib21","first-page":"571","article-title":"Bar workers' exposure to second-hand smoke: the effect of Scottish smoke-free legislation on occupational exposure","volume":"51","author":"Semple","year":"2007","journal-title":"Ann Occup Hyg"},{"issue":"12","key":"2021122118042518300_bib22","doi-asserted-by":"crossref","first-page":"127001","DOI":"10.1289\/EHP3664","article-title":"Exposure to secondhand smoke and arrhythmogenic cardiac alternans in a mouse model","volume":"126","author":"Wang","year":"2018","journal-title":"Environ Health Perspect"},{"issue":"31","key":"2021122118042518300_bib23","doi-asserted-by":"crossref","first-page":"11112","DOI":"10.1073\/pnas.0500360102","article-title":"Constitutively active L-type Ca2+ channels","volume":"102","author":"Navedo","year":"2005","journal-title":"Proc Natl Acad Sci"},{"issue":"4","key":"2021122118042518300_bib24","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1161\/CIRCRESAHA.109.213363","article-title":"Increased coupled gating of L-type Ca2+ channels during hypertension and Timothy syndrome","volume":"106","author":"Navedo","year":"2010","journal-title":"Circ Res"},{"issue":"5","key":"2021122118042518300_bib25","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1085\/jgp.95.5.911","article-title":"Permeation in the dihydropyridine-sensitive calcium channel. Multi-ion occupancy but no anomalous mole-fraction effect between Ba2+ and Ca2+","volume":"95","author":"Yue","year":"1990","journal-title":"J Gen Physiol"},{"issue":"50","key":"2021122118042518300_bib26","doi-asserted-by":"crossref","first-page":"39710","DOI":"10.1074\/jbc.M005462200","article-title":"Protein phosphatase 2A is associated with class C L-type calcium channels (Cav1.2) and antagonizes channel phosphorylation by cAMP-dependent protein kinase","volume":"275","author":"Davare","year":"2000","journal-title":"J Biol Chem"},{"key":"2021122118042518300_bib27","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.12688\/f1000research.11808.1","article-title":"Proteolytic processing of the L-type Ca (2+) channel alpha 11.2 subunit in neurons","volume":"6","author":"Buonarati","year":"2017","journal-title":"F1000Res"},{"issue":"1","key":"2021122118042518300_bib28","doi-asserted-by":"crossref","first-page":"139","DOI":"10.15252\/embj.201695709","article-title":"Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy","volume":"37","author":"Richter","year":"2018","journal-title":"EMBO J"},{"issue":"1","key":"2021122118042518300_bib29","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/bs.apha.2016.08.002","article-title":"Calcium channels in vascular smooth muscle","volume":"78","author":"Ghosh","year":"2017","journal-title":"Adv Pharmacol"},{"issue":"4","key":"2021122118042518300_bib30","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/micc.12021","article-title":"Local regulation of L-type ca(2+) channel sparklets in arterial smooth muscle","volume":"20","author":"Navedo","year":"2013","journal-title":"Microcirculation"},{"issue":"5","key":"2021122118042518300_bib31","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1073\/pnas.1116731109","article-title":"Calcium signaling amplification by oligomerization of L-type Cav1.2 channels","volume":"109","author":"Dixon","year":"2012","journal-title":"Proc Natl Acad Sci"},{"key":"2021122118042518300_bib32","doi-asserted-by":"publisher","first-page":"e05608","DOI":"10.7554\/eLife.05608","article-title":"Graded Ca(2)(+)\/calmodulin-dependent coupling of voltage-gated CaV1.2 channels","volume":"4","author":"Dixon","year":"2015","journal-title":"eLife"},{"issue":"1","key":"2021122118042518300_bib33","doi-asserted-by":"crossref","first-page":"e58","DOI":"10.1002\/cpim.58","article-title":"Proximity Ligation Assay (PLA)","volume":"123","author":"Alam","year":"2018","journal-title":"Curr Protoc Immunol"},{"issue":"42","key":"2021122118042518300_bib34","doi-asserted-by":"crossref","first-page":"72127","DOI":"10.18632\/oncotarget.19552","article-title":"Visualization and quantitation of epidermal growth factor receptor homodimerization and activation with a proximity ligation assay","volume":"8","author":"Ota","year":"2017","journal-title":"Oncotarget"},{"issue":"1","key":"2021122118042518300_bib35","doi-asserted-by":"crossref","first-page":"e12423","DOI":"10.1111\/micc.12423","article-title":"Regulation of voltage-gated potassium channels in vascular smooth muscle during hypertension and metabolic disorders","volume":"25","author":"Nieves-Cintron","year":"2018","journal-title":"Microcirculation"},{"issue":"45","key":"2021122118042518300_bib36","doi-asserted-by":"crossref","first-page":"47326","DOI":"10.1074\/jbc.M408789200","article-title":"NFATc3 regulates Kv2.1 expression in arterial smooth muscle","volume":"279","author":"Amberg","year":"2004","journal-title":"J Biol Chem"},{"issue":"1","key":"2021122118042518300_bib37","doi-asserted-by":"crossref","first-page":"14058","DOI":"10.1038\/s41598-017-14565-9","article-title":"Impaired BKCa channel function in native vascular smooth muscle from humans with type 2 diabetes","volume":"7","author":"Nieves-Cintron","year":"2017","journal-title":"Sci Rep"},{"issue":"1","key":"2021122118042518300_bib38","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1469-7793.2001.00049.x","article-title":"Rapidly inactivating and non-inactivating calcium-activated potassium currents in frog saccular hair cells","volume":"536","author":"Armstrong","year":"2001","journal-title":"J Physiol"},{"issue":"5","key":"2021122118042518300_bib39","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.1074\/jbc.M608822200","article-title":"Activation of NFATc3 down-regulates the \u03b21 subunit of large conductance, calcium-activated K+ channels in arterial smooth muscle and contributes to hypertension","volume":"282","author":"Nieves-Cintr\u00f3n","year":"2007","journal-title":"J Biol Chem"},{"issue":"3","key":"2021122118042518300_bib40","doi-asserted-by":"crossref","first-page":"C611","DOI":"10.1152\/ajpcell.00435.2007","article-title":"NFATc3 regulates BK channel function in murine urinary bladder smooth muscle","volume":"295","author":"Layne","year":"2008","journal-title":"Am J Physiol Cell Physiol"},{"issue":"1","key":"2021122118042518300_bib41","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1146\/annurev.immunol.15.1.707","article-title":"Transcription factors of the NFAT family: regulation and function","volume":"15","author":"Rao","year":"1997","journal-title":"Annu Rev Immunol"},{"issue":"40","key":"2021122118042518300_bib42","doi-asserted-by":"crossref","first-page":"15623","DOI":"10.1073\/pnas.0808759105","article-title":"The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension","volume":"105","author":"Nieves-Cintron","year":"2008","journal-title":"Proc Natl Acad Sci"},{"issue":"5","key":"2021122118042518300_bib43","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/j.yjmcc.2012.08.005","article-title":"Ca(2+) influx through L-type Ca(2+) channels and transient receptor potential channels activates pathological hypertrophy signaling","volume":"53","author":"Gao","year":"2012","journal-title":"J Mol Cell Cardiol"},{"issue":"2","key":"2021122118042518300_bib44","doi-asserted-by":"crossref","first-page":"C211","DOI":"10.1152\/ajpcell.00267.2009","article-title":"Elevated Ca2+ sparklet activity during acute hyperglycemia and diabetes in cerebral arterial smooth muscle cells","volume":"298","author":"Navedo","year":"2010","journal-title":"Am J Physiol Cell Physiol"},{"issue":"10","key":"2021122118042518300_bib45","doi-asserted-by":"crossref","first-page":"7979","DOI":"10.1074\/jbc.M109466200","article-title":"Up-regulation of L-type voltage-dependent calcium channels after long term exposure to nicotine in cerebral cortical neurons","volume":"277","author":"Katsura","year":"2002","journal-title":"J Biol Chem"},{"issue":"7","key":"2021122118042518300_bib46","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1289\/ehp.112-1241994","article-title":"Metal particulate matter components affect gene expression and beat frequency of neonatal rat ventricular myocytes","volume":"112","author":"Graff","year":"2004","journal-title":"Environ Health Perspect"},{"issue":"1","key":"2021122118042518300_bib47","doi-asserted-by":"crossref","first-page":"G7","DOI":"10.1152\/ajpgi.00205.2004","article-title":"Carbon monoxide activates human intestinal smooth muscle L-type Ca2+ channels through a nitric oxide-dependent mechanism","volume":"288","author":"Lim","year":"2005","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"9","key":"2021122118042518300_bib48","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1113\/expphysiol.2011.059063","article-title":"Ion channels as target effectors for carbon monoxide","volume":"96","author":"Peers","year":"2011","journal-title":"Exp Physiol"},{"issue":"10","key":"2021122118042518300_bib49","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1097\/HJH.0000000000002478","article-title":"Association between secondhand smoke exposure and hypertension: nearly as large as smoking","volume":"38","author":"Skipina","year":"2020","journal-title":"J Hypertens"}],"container-title":["Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/full\/10.1093\/function\/zqab041","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1093\/function\/zqab041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:06:44Z","timestamp":1781629604000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/full\/10.1093\/function\/zqab041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,9,17]]}},"URL":"https:\/\/doi.org\/10.1093\/function\/zqab041","relation":{},"ISSN":["2633-8823"],"issn-type":[{"value":"2633-8823","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021]]},"published":{"date-parts":[[2021]]},"article-number":"zqab041"}}