{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:53:29Z","timestamp":1781632409115,"version":"3.54.5"},"reference-count":44,"publisher":"American Physiological Society","issue":"3","license":[{"start":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T00:00:00Z","timestamp":1651708800000},"content-version":"vor","delay-in-days":124,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL133832"],"award-info":[{"award-number":["R01HL133832"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01-HL141084"],"award-info":[{"award-number":["P01-HL141084"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-HL126825"],"award-info":[{"award-number":["R01-HL126825"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-HL153835"],"award-info":[{"award-number":["R01-HL153835"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL147263"],"award-info":[{"award-number":["HL147263"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\u03b2-adrenergic receptor (\u03b2-AR) signaling in cardiac myocytes is central to cardiac function, but spatiotemporal activation within myocytes is unresolved. In rabbit ventricular myocytes, \u03b2-AR agonists or high extracellular [Ca] were applied locally at one end, to measure \u03b2-AR signal propagation as Ca\u2212transient (CaT) amplitude and sarcoplasmic reticulum (SR) Ca uptake. High local [Ca]o, increased CaT amplitude under the pipette faster than did ISO, but was also more spatially restricted. Local isoproterenol (ISO) or norepinephrine (NE) increased CaT amplitude and SR Ca uptake, that spread along the myocyte to the unexposed end. Thus, local [Ca]i decline kinetics reflect spatio-temporal progression of \u03b2-AR end-effects in myocytes. To test whether intracellular \u03b2-ARs contribute to this response, we used \u03b2-AR-blockers that are membrane permeant (propranolol) or not (sotalol). Propranolol completely blocked NE-dependent CaT effects. However, blocking surface \u03b2-ARs only (sotalol) suppressed only \u223c50% of the NE-induced increase in CaT peak and rate of [Ca]i decline, but these changes spread more gradually than NE alone. We also tested whether A-kinase anchoring protein 7\u03b3 (AKAP7\u03b3; that interacts with phospholamban) is mobile, such that it might contribute to intracellular spatial propagation of \u03b2-AR signaling. We found AKAP7\u03b3 to be highly mobile using fluorescence recovery after photobleach of GFP tagged AKAP7\u03b3, and that PKA activation accelerated AKAP7\u03b3-GFP wash-out upon myocyte saponin-permeabilization, suggesting increased AKAP7\u03b3 mobility. We conclude that local \u03b2-AR activation can activate SR Ca uptake at remote myocyte sites, and that intracellular \u03b2-AR and AKAP7\u03b3 mobility may play a role in this spread of activation.<\/jats:p>","DOI":"10.1093\/function\/zqac020","type":"journal-article","created":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T18:08:06Z","timestamp":1651774086000},"source":"Crossref","is-referenced-by-count":13,"title":["Subcellular Propagation of Cardiomyocyte \u03b2-Adrenergic Activation of Calcium Uptake Involves Internal \u03b2-Receptors and AKAP7"],"prefix":"10.1152","volume":"3","author":[{"given":"Thomas R","family":"Shannon","sequence":"first","affiliation":[{"name":"Department of Molecular Biophysics and Physiology, Rush University, Chicago, IL 60605, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan J","family":"Bare","sequence":"additional","affiliation":[{"name":"Department of Molecular Biophysics and Physiology, Rush University, Chicago, IL 60605, 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