{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:47:42Z","timestamp":1788385662324,"version":"build-2803163510"},"reference-count":50,"publisher":"American Physiological Society","issue":"1","license":[{"start":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T00:00:00Z","timestamp":1701734400000},"content-version":"vor","delay-in-days":12,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-AG080731-01"],"award-info":[{"award-number":["R01-AG080731-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R00AG057825"],"award-info":[{"award-number":["R00AG057825"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01-AG080047"],"award-info":[{"award-number":["R01-AG080047"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alzheimer\u2019s disease (AD) develops along a continuum that spans years prior to diagnosis. Decreased muscle function and mitochondrial respiration occur years earlier in those that develop AD; however, it is unknown what causes these peripheral phenotypes in a disease of the brain. Exercise promotes muscle, mitochondria, and cognitive health and is proposed to be a potential therapeutic for AD, but no study has investigated how skeletal muscle adapts to exercise training in an AD-like context. Utilizing 5xFAD mice, an AD model that develops ad-like pathology and cognitive impairments around 6\u2009mo of age, we examined in vivo neuromuscular function and exercise adapations (mitochondrial respiration and RNA sequencing) before the manifestation of overt cognitive impairment. We found 5xFAD mice develop neuromuscular dysfunction beginning as early as 4\u2009mo of age, characterized by impaired nerve-stimulated muscle torque production and compound nerve action potential of the sciatic nerve. Furthermore, skeletal muscle in 5xFAD mice had altered, sex-dependent, adaptive responses (mitochondrial respiration and gene expression) to exercise training in the absence of overt cognitive impairment. Changes in peripheral systems, specifically neural communication to skeletal muscle, may be harbingers for AD and have implications for lifestyle interventions, like exercise, in AD.<\/jats:p>","DOI":"10.1093\/function\/zqad066","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T05:27:44Z","timestamp":1701149264000},"source":"Crossref","is-referenced-by-count":16,"title":["Neuromuscular Dysfunction Precedes Cognitive Impairment in a Mouse Model of Alzheimer\u2019s Disease"],"prefix":"10.1152","volume":"5","author":[{"given":"Matthew H","family":"Brisendine","sequence":"first","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna S","family":"Nichenko","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aloka B","family":"Bandara","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orion S","family":"Willoughby","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niloufar","family":"Amiri","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zach","family":"Weingrad","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyn S","family":"Specht","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M","family":"Bond","sequence":"additional","affiliation":[{"name":"Translational Biology, Medicine, and Health Program, Virginia Tech , Roanoke, VA 24016 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele","family":"Addington","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Ronald G","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Health, Human Performance, and Recreation, University of Arkansas , Fayetteville, AR 72701 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A","family":"Murach","sequence":"additional","affiliation":[{"name":"Department of Health, Human Performance, and Recreation, University of Arkansas , Fayetteville, AR 72701 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Poelzing","sequence":"additional","affiliation":[{"name":"Translational Biology, Medicine, and Health Program, Virginia Tech , Roanoke, VA 24016 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siobhan M","family":"Craige","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"},{"name":"Translational Biology, Medicine, and Health Program, Virginia Tech , Roanoke, VA 24016 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W","family":"Grange","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6658-4975","authenticated-orcid":false,"given":"Joshua C","family":"Drake","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods, and Exercise, Virginia Tech , Blacksburg, VA 24061 , USA"},{"name":"Translational Biology, Medicine, and Health Program, Virginia Tech , Roanoke, VA 24016 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","published-online":{"date-parts":[[2023,12,4]]},"reference":[{"issue":"3","key":"2024031307152313000_bib1","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.jalz.2011.03.003","article-title":"Toward defining the preclinical stages of Alzheimer\u2019s disease: recommendations from the National Institute on Aging-Alzheimer\u2019s Association workgroups on diagnostic guidelines for Alzheimer\u2019s disease","volume":"7","author":"Sperling","year":"2011","journal-title":"Alzheimers Dement"},{"issue":"5","key":"2024031307152313000_bib2","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1001\/archneur.1995.00540290075020","article-title":"The \u201cpreclinical phase\u201d of probable Alzheimer\u2019s Disease: a 13-year prospective study of the Framingham cohort","volume":"52","author":"Linn","year":"1995","journal-title":"Arch Neurol"},{"issue":"8","key":"2024031307152313000_bib3","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/S1474-4422(14)70136-X","article-title":"Potential for primary prevention of Alzheimer\u2019s disease: an analysis of population-based data","volume":"13","author":"Norton","year":"2014","journal-title":"Lancet Neurol"},{"issue":"7","key":"2024031307152313000_bib4","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1002\/alz.12940","article-title":"Examination of potentially modifiable dementia risk factors across the adult life course: the Framingham Heart Study","volume":"19","author":"Hwang","year":"2023","journal-title":"Alzheimers Dement"},{"issue":"4","key":"2024031307152313000_bib5","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1001\/archneurol.2010.38","article-title":"Reduced lean mass in early Alzheimer disease and its association with brain atrophy","volume":"67","author":"Burns","year":"2010","journal-title":"Arch Neurol"},{"issue":"3","key":"2024031307152313000_bib6","doi-asserted-by":"crossref","first-page":"647","DOI":"10.3233\/JAD-2011-110713","article-title":"Weight loss and rapid cognitive decline in community-dwelling patients with Alzheimer\u2019s disease","volume":"28","author":"Soto","year":"2012","journal-title":"J Alzheimers Dis"},{"issue":"710","key":"2024031307152313000_bib7","first-page":"1","article-title":"Sarcopenia and muscle functions at various stages of Alzheimer Disease","volume":"9","author":"Ogawa","year":"2018","journal-title":"Front Neurol"},{"issue":"2","key":"2024031307152313000_bib8","doi-asserted-by":"crossref","first-page":"e13552","DOI":"10.1111\/acel.13552","article-title":"Muscle mitochondrial energetics predicts mobility decline in well-functioning older adults: the baltimore longitudinal study of aging","volume":"21","author":"Tian","year":"2022","journal-title":"Aging Cell"},{"issue":"6","key":"2024031307152313000_bib9","doi-asserted-by":"crossref","first-page":"zqab045","DOI":"10.1093\/function\/zqab045","article-title":"Mild cognitive impairment and donepezil impact mitochondrial respiratory capacity in skeletal muscle","volume":"2","author":"Morris","year":"2021","journal-title":"Function"},{"issue":"3","key":"2024031307152313000_bib10","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1152\/japplphysiol.00659.2022","article-title":"Early stage Alzheimer\u2019s disease: are skeletal muscle and exercise the key?","volume":"134","author":"Brisendine","year":"2023","journal-title":"J Appl Physiol (1985)"},{"issue":"1","key":"2024031307152313000_bib11","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s13195-019-0538-4","article-title":"Midlife physical activity is associated with lower incidence of vascular dementia but not Alzheimer\u2019s disease","volume":"11","author":"Hansson","year":"2019","journal-title":"Alzheimers Res Ther"},{"issue":"12","key":"2024031307152313000_bib12","doi-asserted-by":"crossref","first-page":"e1322","DOI":"10.1212\/WNL.0000000000007021","article-title":"Cognitive and physical activity and dementia: a 44-year longitudinal population study of women","volume":"92","author":"Najar","year":"2019","journal-title":"Neurology"},{"issue":"6406","key":"2024031307152313000_bib13","doi-asserted-by":"crossref","first-page":"eaan8821","DOI":"10.1126\/science.aan8821","article-title":"Combined adult neurogenesis and BDNF mimic exercise effects on cognition in an Alzheimer\u2019s mouse model","volume":"361","author":"Choi","year":"2018","journal-title":"Science"},{"issue":"1","key":"2024031307152313000_bib14","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1038\/s41598-020-58309-8","article-title":"Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer\u2019s disease","volume":"10","author":"Svensson","year":"2020","journal-title":"Sci Rep"},{"issue":"11","key":"2024031307152313000_bib15","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1001\/archneurol.2009.240","article-title":"Association of muscle strength with the risk of Alzheimer disease and the rate of cognitive decline in community-dwelling older persons","volume":"66","author":"Boyle","year":"2009","journal-title":"Arch Neurol"},{"issue":"1-2","key":"2024031307152313000_bib16","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1159\/000109498","article-title":"Grip strength and the risk of incident Alzheimer\u2019s disease","volume":"29","author":"Buchman","year":"2007","journal-title":"Neuroepidemiology"},{"issue":"12","key":"2024031307152313000_bib17","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1001\/archneur.63.12.1763","article-title":"Motor dysfunction in mild cognitive impairment and the risk of incident Alzheimer disease","volume":"63","author":"Aggarwal","year":"2006","journal-title":"Arch Neurol"},{"issue":"1","key":"2024031307152313000_bib18","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41597-021-01054-y","article-title":"Systematic phenotyping and characterization of the 5xFAD mouse model of Alzheimer\u2019s disease","volume":"8","author":"Forner","year":"2021","journal-title":"Sci Data"},{"issue":"40","key":"2024031307152313000_bib19","doi-asserted-by":"crossref","first-page":"10129","DOI":"10.1523\/JNEUROSCI.1202-06.2006","article-title":"Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer\u2019s disease mutations: potential factors in amyloid plaque formation","volume":"26","author":"Oakley","year":"2006","journal-title":"J Neurosci"},{"issue":"3","key":"2024031307152313000_bib20","doi-asserted-by":"crossref","first-page":"e12538","DOI":"10.1111\/gbb.12538","article-title":"Age-related deterioration of motor function in male and female 5xFAD mice from 3 to 16 months of age","volume":"19","author":"O'Leary","year":"2020","journal-title":"Genes Brain Behav"},{"key":"2024031307152313000_bib21","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.freeradbiomed.2018.02.006","article-title":"Mitochondrial protein S-nitrosation protects against ischemia reperfusion-induced denervation at neuromuscular junction in skeletal muscle","volume":"117","author":"Wilson","year":"2018","journal-title":"Free Radic Biol Med"},{"issue":"6","key":"2024031307152313000_bib22","doi-asserted-by":"crossref","first-page":"7330","DOI":"10.1096\/fj.201903055R","article-title":"A novel voluntary weightlifting model in mice promotes muscle adaptation and insulin sensitivity with simultaneous enhancement of autophagy and mTOR pathway","volume":"34","author":"Cui","year":"2020","journal-title":"FASEB J"},{"issue":"1","key":"2024031307152313000_bib23","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1152\/japplphysiol.00358.2018","article-title":"Voluntary running protects against neuromuscular dysfunction following hindlimb ischemia-reperfusion in mice","volume":"126","author":"Wilson","year":"2019","journal-title":"J Appl Physiol"},{"issue":"11","key":"2024031307152313000_bib24","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1016\/j.clinph.2013.04.015","article-title":"Electrical stimulation site influences the spatial distribution of motor units recruited in tibialis anterior","volume":"124","author":"Okuma","year":"2013","journal-title":"Clin Neurophysiol"},{"issue":"876816","key":"2024031307152313000_bib25","first-page":"1","article-title":"Age related changes in muscle mass and force generation in the triple transgenic (3xTgAD) mouse model of Alzheimer\u2019s disease","volume":"14","author":"Xu","year":"2022","journal-title":"Front Aging Neurosci"},{"issue":"8","key":"2024031307152313000_bib26","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1002\/lary.26531","article-title":"Compound motor action potential duration and latency are markers of recurrent laryngeal nerve injury","volume":"127","author":"Bhatt","year":"2017","journal-title":"Laryngoscope"},{"issue":"1","key":"2024031307152313000_bib27","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1038\/s41467-017-00520-9","article-title":"Ampk phosphorylation of Ulk1 is required for targeting of mitochondria to lysosomes in exercise-induced mitophagy","volume":"8","author":"Laker","year":"2017","journal-title":"Nat Commun"},{"issue":"876816","key":"2024031307152313000_bib28","first-page":"1","article-title":"Ulk1, Not Ulk2, is required for exercise training-induced improvement of insulin response in skeletal muscle","volume":"12","author":"Drake","year":"2021","journal-title":"Front Physiol"},{"issue":"1","key":"2024031307152313000_bib29","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nprot.2006.2","article-title":"T-maze alternation in the rodent","volume":"1","author":"Deacon","year":"2006","journal-title":"Nat Protoc"},{"issue":"37","key":"2024031307152313000_bib30","doi-asserted-by":"crossref","first-page":"e2025932118","DOI":"10.1073\/pnas.2025932118","article-title":"Mitochondria-localized AMPK responds to local energetics and contributes to exercise and energetic stress-induced mitophagy","volume":"118","author":"Drake","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"2024031307152313000_bib31","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1038\/nprot.2008.61","article-title":"Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells","volume":"3","author":"Kuznetsov","year":"2008","journal-title":"Nat Protoc"},{"issue":"4","key":"2024031307152313000_bib32","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.2337\/db12-1219","article-title":"Methods for assessing mitochondrial function in diabetes","volume":"62","author":"Perry","year":"2013","journal-title":"Diabetes"},{"issue":"1","key":"2024031307152313000_bib33","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1152\/japplphysiol.00358.2018","article-title":"Voluntary running protects against neuromuscular dysfunction following hindlimb ischemia-reperfusion in mice","volume":"126","author":"Wilson","year":"2019","journal-title":"J Appl Physiol (1985)"},{"key":"2024031307152313000_bib34","doi-asserted-by":"crossref","first-page":"231","DOI":"10.2147\/NDT.S349005","article-title":"Reduced peripheral nerve conduction velocity is associated with Alzheimer\u2019s Disease: a cross-sectional study from China","volume":"18","author":"Qian","year":"2022","journal-title":"Neuropsychiatr Dis Treat"},{"issue":"10","key":"2024031307152313000_bib35","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.1096\/fj.201500100R","article-title":"Mitochondrial adaptations to high-volume exercise training are rapidly reversed after a reduction in training volume in human skeletal muscle","volume":"30","author":"Granata","year":"2016","journal-title":"FASEB J"},{"key":"2024031307152313000_bib36","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.neuroscience.2013.06.047","article-title":"Regulation of conduction time along axons","volume":"276","author":"Seidl","year":"2014","journal-title":"Neuroscience"},{"issue":"32","key":"2024031307152313000_bib37","first-page":"2507","article-title":"Morphological differences in skeletal muscle atrophy of rats with motor nerve and\/or sensory nerve injury","volume":"7","author":"Zhao","year":"2012","journal-title":"Neural Regen Res"},{"issue":"923190","key":"2024031307152313000_bib38","first-page":"1","article-title":"Changes of gene expression patterns of muscle pathophysiology-related transcription factors during denervated muscle atrophy","volume":"13","author":"Yang","year":"2022","journal-title":"Front Physiol"},{"issue":"2","key":"2024031307152313000_bib39","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0022-510X(77)90069-7","article-title":"The numbers of limb motor neurons in the human lumbosacral cord throughout life","volume":"34","author":"Tomlinson","year":"1977","journal-title":"J Neurol Sci"},{"key":"2024031307152313000_bib40","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.exger.2015.07.007","article-title":"Motor unit loss is accompanied by decreased peak muscle power in the lower limb of older adults","volume":"70","author":"McKinnon","year":"2015","journal-title":"Exp Gerontol"},{"key":"2024031307152313000_bib41","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.neurobiolaging.2018.03.016","article-title":"Muscle strength and size are associated with motor unit connectivity in aged mice","volume":"67","author":"Sheth","year":"2018","journal-title":"Neurobiol Aging"},{"issue":"12","key":"2024031307152313000_bib42","doi-asserted-by":"crossref","first-page":"107808","DOI":"10.1016\/j.celrep.2020.107808","article-title":"Skeletal muscle transcriptomic comparison between long-term trained and untrained men and women","volume":"31","author":"Chapman","year":"2020","journal-title":"Cell Rep"},{"issue":"10","key":"2024031307152313000_bib43","doi-asserted-by":"crossref","first-page":"4436","DOI":"10.1002\/alz.13388","article-title":"Skeletal muscle mitochondrial function predicts cognitive impairment and is associated with biomarkers of Alzheimer's disease and neurodegeneration","volume":"19","author":"Tian","year":"2023","journal-title":"Alzheimers Dement"},{"issue":"7","key":"2024031307152313000_bib44","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1093\/brain\/awac177","article-title":"Sex differences in the genetic architecture of cognitive resilience to Alzheimer\u2019s disease","volume":"145","author":"Eissman","year":"2022","journal-title":"Brain J Neurol"},{"issue":"24","key":"2024031307152313000_bib45","doi-asserted-by":"crossref","first-page":"7361","DOI":"10.1113\/JP272487","article-title":"Denervation drives mitochondrial dysfunction in skeletal muscle of octogenarians","volume":"594","author":"Spendiff","year":"2016","journal-title":"J Physiol"},{"key":"2024031307152313000_bib46","doi-asserted-by":"crossref","first-page":"111613","DOI":"10.1016\/j.exger.2021.111613","article-title":"Variation in muscle and neuromuscular junction morphology between atrophy-resistant and atrophy-prone muscles supports failed re-innervation in aging muscle atrophy","volume":"156","author":"Burke","year":"2021","journal-title":"Exp Gerontol"},{"issue":"4","key":"2024031307152313000_bib47","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1152\/japplphysiol.00149.2016","article-title":"Motor unit number and transmission stability in octogenarian world class athletes: can age-related deficits be outrun?","volume":"121","author":"Power","year":"2016","journal-title":"J Appl Physiol (1985)"},{"issue":"1","key":"2024031307152313000_bib48","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3233\/JAD-201100","article-title":"Cognitive effects of aerobic exercise in Alzheimer\u2019s Disease: a pilot randomized controlled trial","volume":"80","author":"Yu","year":"2021","journal-title":"J Alzheimers Dis"},{"issue":"2","key":"2024031307152313000_bib49","doi-asserted-by":"crossref","first-page":"e0170547","DOI":"10.1371\/journal.pone.0170547","article-title":"Aerobic exercise for Alzheimer\u2019s disease: a randomized controlled pilot trial","volume":"12","author":"Morris","year":"2017","journal-title":"PLoS One"},{"issue":"5","key":"2024031307152313000_bib50","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.cmet.2021.02.017","article-title":"Excessive exercise training causes mitochondrial functional impairment and decreases glucose tolerance in healthy volunteers","volume":"33","author":"Flockhart","year":"2021","journal-title":"Cell Metab"}],"container-title":["Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/full\/10.1093\/function\/zqad066","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1093\/function\/zqad066","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:07:07Z","timestamp":1781629627000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/full\/10.1093\/function\/zqad066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,23]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,11,23]]}},"URL":"https:\/\/doi.org\/10.1093\/function\/zqad066","relation":{},"ISSN":["2633-8823"],"issn-type":[{"value":"2633-8823","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024]]},"published":{"date-parts":[[2023,11,23]]},"article-number":"zqad066"}}