{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:53:56Z","timestamp":1781632436276,"version":"3.54.5"},"reference-count":65,"publisher":"American Physiological Society","issue":"5","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":203,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Ru 368\/5-4"],"award-info":[{"award-number":["Ru 368\/5-4"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010215","name":"Deutsche Diabetes Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010215","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The consensus or canonical model of glucose-stimulated insulin secretion provides that the metabolism of glucose closes KATP channels by increase of the ATP\/ADP ratio and that the ensuing depolarization-induced Ca2+ influx through voltage-dependent Ca2+ channels represents the immediate signal for the onset of exocytosis. However, it has been shown earlier that the depolarization-induced secretion can be suppressed by inhibition of the oxidative phosphorylation, pointing to an energy-requiring step presumably located downstream of Ca2+ influx. Here, we have investigated the relation between oxidative phosphorylation and the insulinotropic effect of K+ depolarization to better localize the energy-requiring step. The specific inhibitor of the mitochondrial F1FO ATPase, oligomycin, concentration-dependently and time-dependently inhibited the insulin secretion elicited by a strong K+ depolarization (40\u00a0mm). Perifusion with 4\u00a0\u00b5g\/mL of oligomycin for 20, 10, or 5\u00a0min prior to the K+ depolarization reduced the amount of insulin secreted from freshly isolated islets from control value to about 5% with a half-time of 1.6\u00a0min. 0.4\u00a0\u00b5g\/mL of oligomycin required more time for comparable effects. Cultured islets were less susceptible to the inhibitory action of oligomycin than fresh islets, corresponding to their significantly higher ATP\/ADP ratio. The perifusion with oligomycin prior to the K+ depolarization did not decrease the depolarization-elevated cytosolic Ca2+ concentration and did not affect the resting plasma membrane potential and the extent of depolarization by 40\u00a0mm KCl. In conclusion, the exocytotic machinery of the beta cell requires a continuously running oxidative phosphorylation to remain responsive to the Ca2+ signal for granule fusion.<\/jats:p>","DOI":"10.1093\/function\/zqaf033","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T11:50:08Z","timestamp":1753271408000},"source":"Crossref","is-referenced-by-count":1,"title":["The Amount of Releasable Insulin Depends on Continuous Oxidative Phosphorylation"],"prefix":"10.1152","volume":"6","author":[{"given":"Carolin","family":"Tappe","sequence":"first","affiliation":[{"name":"Technische Universit\u00e4t Braunschweig Institute of Pharmacology, Toxicology and Clinical Pharmacy, , D-38106 Braunschweig ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0603-9726","authenticated-orcid":false,"given":"Manjitha","family":"Parambath","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Braunschweig Institute of Pharmacology, Toxicology and Clinical Pharmacy, , D-38106 Braunschweig ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Reschke","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Braunschweig Institute of Pharmacology, Toxicology and Clinical Pharmacy, , D-38106 Braunschweig ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo","family":"Rustenbeck","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Braunschweig Institute of Pharmacology, Toxicology and Clinical Pharmacy, , D-38106 Braunschweig ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","published-online":{"date-parts":[[2025,7,23]]},"reference":[{"issue":"1","key":"2025091915585318000_bib1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1042\/bj0930066","article-title":"Regulation of insulin secretion studied with pieces of rabbit pancreas incubated in vitro","volume":"93","author":"Coore","year":"1964","journal-title":"Biochem J"},{"issue":"2","key":"2025091915585318000_bib2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0014-5793(72)80801-9","article-title":"Effects of L-leucine and alpha-ketoisocaproic acid upon insulin secretion and metabolism of isolated pancreatic islets","volume":"20","author":"Panten","year":"1972","journal-title":"FEBS Lett"},{"issue":"5983","key":"2025091915585318000_bib3","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1038\/311271a0","article-title":"Intracellular ATP directly blocks K+ channels in pancreatic B-cells","volume":"311","author":"Cook","year":"1984","journal-title":"Nature"},{"issue":"2","key":"2025091915585318000_bib4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0079-6107(89)90013-8","article-title":"Electrophysiology of the pancreatic beta-cell","volume":"54","author":"Ashcroft","year":"1989","journal-title":"Prog Biophys Mol Biol"},{"issue":"2","key":"2025091915585318000_bib5","first-page":"123","article-title":"The role of Ca2+ in the release of pancreatic islet hormones","volume":"20","author":"Hellman","year":"1994","journal-title":"Diabete Metab"},{"issue":"3","key":"2025091915585318000_bib6","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1113\/jphysiol.2005.102368","article-title":"Characterization of voltage-dependent sodium and calcium channels in mouse pancreatic A- and B-cells","volume":"572","author":"Vignali","year":"2006","journal-title":"J Physiol"},{"issue":"6","key":"2025091915585318000_bib7","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1172\/JCI88491","article-title":"Ca2+ channel clustering with insulin-containing granules is disturbed in type 2 diabetes","volume":"127","author":"Gandasi","year":"2017","journal-title":"J Clin Invest"},{"issue":"1","key":"2025091915585318000_bib8","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1210\/endo-87-1-84","article-title":"Energy requirements for insulin release from rat pancreas in vitro","volume":"87","author":"Aleyassine","year":"1970","journal-title":"Endocrinology"},{"issue":"3","key":"2025091915585318000_bib9","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/BF01995702","article-title":"Interaction of diazoxide, tolbutamide and ATP4- on nucleotide-dependent K+ channels in an insulin-secreting cell line","volume":"99","author":"Dunne","year":"1987","journal-title":"J. Membrain Biol."},{"issue":"5","key":"2025091915585318000_bib10","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1254\/jjp.27.679","article-title":"Effect of diltiazem on insulin secretion. I. Experiments in vitro","volume":"27","author":"Yamaguchi","year":"1977","journal-title":"Jpn J Pharmacol"},{"issue":"3","key":"2025091915585318000_bib11","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/BF00515566","article-title":"The dihydropyridine derivative, Bay K 8644, enhances insulin secretion by isolated pancreatic islets","volume":"328","author":"Panten","year":"1985","journal-title":"Naunyn-Schmiedeberg's Arch. Pharmacol"},{"issue":"47","key":"2025091915585318000_bib12","doi-asserted-by":"crossref","first-page":"12696","DOI":"10.1002\/anie.201406359","article-title":"The molecular machinery of neurotransmitter release (Nobel lecture)","volume":"53","author":"S\u00fcdhof","year":"2014","journal-title":"Angew Chem Int Ed Engl"},{"issue":"1-2","key":"2025091915585318000_bib13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s00424-002-0781-5","article-title":"Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells","volume":"444","author":"Olofsson","year":"2002","journal-title":"Pfl\u00fcgers Arch\u2014Eur J Physiol"},{"issue":"suppl_1","key":"2025091915585318000_bib14","doi-asserted-by":"crossref","first-page":"S74","DOI":"10.2337\/diabetes.51.2007.S74","article-title":"A subset of 50 secretory granules in close contact with L-type Ca2+ channels accounts for first-phase insulin secretion in mouse beta-cells","volume":"51","author":"Barg","year":"2002","journal-title":"Diabetes"},{"issue":"8","key":"2025091915585318000_bib15","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1007\/s00125-003-1153-1","article-title":"Insulin granule dynamics in pancreatic beta cells","volume":"46","author":"Rorsman","year":"2003","journal-title":"Diabetologia"},{"issue":"9","key":"2025091915585318000_bib16","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.2337\/diabetes.48.9.1686","article-title":"Identification of the docked granule pool responsible for the first phase of glucose-stimulated insulin secretion","volume":"48","author":"Daniel","year":"1999","journal-title":"Diabetes"},{"issue":"3","key":"2025091915585318000_bib17","doi-asserted-by":"crossref","first-page":"374","DOI":"10.2337\/diabetes.47.3.374","article-title":"Effects of depletion of mitochondrial DNA in metabolism secretion coupling in INS-1 cells","volume":"47","author":"Kennedy","year":"1998","journal-title":"Diabetes"},{"issue":"3","key":"2025091915585318000_bib18","doi-asserted-by":"crossref","first-page":"C565","DOI":"10.1152\/ajpcell.00079.2004","article-title":"Hypothesis: one rate-limiting step controls the magnitude of both phases of glucose-stimulated insulin secretion","volume":"287","author":"Straub","year":"2004","journal-title":"Am J Physiol Cell Physiol"},{"issue":"8","key":"2025091915585318000_bib19","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.2337\/diab.46.8.1305","article-title":"Energetic requirement of insulin secretion distal to calcium influx","volume":"46","author":"Rustenbeck","year":"1997","journal-title":"Diabetes"},{"issue":"3","key":"2025091915585318000_bib20","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1042\/bj2970455","article-title":"Two sites of glucose control of insulin release with distinct dependence on the energy state in pancreatic B-cells","volume":"297","author":"Detimary","year":"1994","journal-title":"Biochem J"},{"issue":"11","key":"2025091915585318000_bib21","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.2337\/diabetes.49.11.1751","article-title":"Triggering and amplifying pathways of regulation of insulin secretion by glucose","volume":"49","author":"Henquin","year":"2000","journal-title":"Diabetes"},{"issue":"6","key":"2025091915585318000_bib22","doi-asserted-by":"crossref","first-page":"355","DOI":"10.3390\/metabo11060355","article-title":"What is the metabolic amplification of insulin secretion and is it (still) relevant?","volume":"11","author":"Rustenbeck","year":"2021","journal-title":"Metabolites"},{"key":"2025091915585318000_bib23","doi-asserted-by":"crossref","first-page":"109085","DOI":"10.1016\/j.mbs.2023.109085","article-title":"Deconstructing the integrated oscillator model for pancreatic \u03b2-cells","volume":"365","author":"Bertram","year":"2023","journal-title":"Math Biosci"},{"issue":"36","key":"2025091915585318000_bib24","doi-asserted-by":"crossref","first-page":"24334","DOI":"10.1074\/jbc.M802097200","article-title":"Islet oxygen consumption and insulin secretion tightly coupled to calcium derived from L-type calcium channels but not from the endoplasmic reticulum","volume":"283","author":"Gilbert","year":"2008","journal-title":"J Biol Chem"},{"issue":"3","key":"2025091915585318000_bib25","doi-asserted-by":"crossref","first-page":"E717","DOI":"10.1152\/ajpendo.00282.2009","article-title":"A highly energetic process couples calcium influx through L-type calcium channels to insulin secretion in pancreatic beta-cells","volume":"297","author":"Jung","year":"2009","journal-title":"Am J Physiol Endocrinol Metab"},{"issue":"2","key":"2025091915585318000_bib26","doi-asserted-by":"crossref","first-page":"E223","DOI":"10.1152\/ajpendo.00010.2012","article-title":"Ca(2+)-dependent desensitization of insulin secretion by strong potassium depolarization","volume":"303","author":"Willenborg","year":"2012","journal-title":"Am J Physiol Endocrinol Metab"},{"issue":"6","key":"2025091915585318000_bib27","doi-asserted-by":"crossref","first-page":"E697","DOI":"10.1152\/ajpendo.00362.2013","article-title":"Insulinotropic effect of high potassium concentration beyond plasma membrane depolarization","volume":"306","author":"Belz","year":"2014","journal-title":"Am J Physiol Endocrinol Metab"},{"issue":"12","key":"2025091915585318000_bib28","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1007\/s00125-005-0030-5","article-title":"Selective loss of glucose-induced amplification of insulin secretion in mouse pancreatic islets pretreated with sulfonylurea in the absence of fuels","volume":"48","author":"Urban","year":"2005","journal-title":"Diabetologia"},{"issue":"1","key":"2025091915585318000_bib29","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0014-5793(90)80667-8","article-title":"Simultaneous recordings of glucose dependent electrical activity and ATP-regulated K(+)-currents in isolated mouse pancreatic beta-cells","volume":"261","author":"Smith","year":"1990","journal-title":"FEBS Lett"},{"issue":"5","key":"2025091915585318000_bib30","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/BF00657506","article-title":"Opposite effects of tolbutamide and diazoxide on the ATP-dependent K+ channel in mouse pancreatic beta-cells","volume":"407","author":"Trube","year":"1986","journal-title":"Pflugers Arch"},{"issue":"2","key":"2025091915585318000_bib31","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0014-5793(88)80925-6","article-title":"Cytosolic ADP enhances the sensitivity to tolbutamide of ATP-dependent K+ channels from pancreatic B-cells","volume":"239","author":"Z\u00fcnkler","year":"1988","journal-title":"FEBS Lett"},{"issue":"20","key":"2025091915585318000_bib32","doi-asserted-by":"crossref","first-page":"17422","DOI":"10.1074\/jbc.M110.202820","article-title":"Reduced cytochrome C is an essential regulator of sustained insulin secretion by pancreatic islets","volume":"286","author":"Jung","year":"2011","journal-title":"J Biol Chem"},{"issue":"50","key":"2025091915585318000_bib33","doi-asserted-by":"crossref","first-page":"14461","DOI":"10.1021\/bi7016816","article-title":"The L-type voltage-gated Ca2+ channel is the Ca2+ sensor protein of stimulus-secretion coupling in pancreatic beta cells","volume":"46","author":"Trus","year":"2007","journal-title":"Biochemistry"},{"issue":"2","key":"2025091915585318000_bib34","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/BF01230690","article-title":"Ultrastructural morphometry of the pancreatic -cell","volume":"9","author":"Dean","year":"1973","journal-title":"Diabetologia"},{"issue":"Suppl. 1","key":"2025091915585318000_bib35","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/dom.13015","article-title":"A 4D view on insulin secretory granule turnover in the \u03b2-cell","volume":"19","author":"M\u00fcller","year":"2017","journal-title":"Diabetes Obes Metab"},{"issue":"Pt 3","key":"2025091915585318000_bib36","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1113\/jphysiol.2003.059675","article-title":"Capacitance measurements of exocytosis in mouse pancreatic alpha-, beta- and delta-cells within intact islets of Langerhans","volume":"556","author":"G\u00f6pel","year":"2004","journal-title":"J Physiol"},{"issue":"5585","key":"2025091915585318000_bib37","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1126\/science.1073806","article-title":"Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet","volume":"297","author":"Takahashi","year":"2002","journal-title":"Science"},{"issue":"6","key":"2025091915585318000_bib38","doi-asserted-by":"crossref","first-page":"673","DOI":"10.2337\/diab.38.6.673","article-title":"A new phase of insulin secretion. How will it contribute to our understanding of beta-cell function?","volume":"38","author":"Grodsky","year":"1989","journal-title":"Diabetes"},{"issue":"s2","key":"2025091915585318000_bib39","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1463-1326.2007.00774.x","article-title":"The balance between proinsulin biosynthesis and insulin secretion: where can imbalance lead?","volume":"9","author":"Uchizono","year":"2007","journal-title":"Diabetes Obes Metab"},{"issue":"4567","key":"2025091915585318000_bib40","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1126\/science.6181562","article-title":"Evidence that glucose \u201cmarks\u201d beta cells resulting in preferential release of newly synthesized insulin","volume":"218","author":"Gold","year":"1982","journal-title":"Science"},{"issue":"5","key":"2025091915585318000_bib41","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1007\/s00018-020-03688-4","article-title":"Insulin granule biogenesis and exocytosis","volume":"78","author":"Omar-Hmeadi","year":"2021","journal-title":"Cell. Mol. Life Sci."},{"issue":"suppl_1","key":"2025091915585318000_bib42","doi-asserted-by":"crossref","first-page":"S60","DOI":"10.2337\/diabetes.51.2007.S60","article-title":"Signals and pools underlying biphasic insulin secretion","volume":"51","author":"Henquin","year":"2002","journal-title":"Diabetes"},{"issue":"1","key":"2025091915585318000_bib43","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/0003-9861(83)90193-5","article-title":"The stimulus-secretion coupling of glucose-induced insulin release: fuel metabolism in islets deprived of exogenous nutrient","volume":"224","author":"Malaisse","year":"1983","journal-title":"Arch Biochem Biophys"},{"key":"2025091915585318000_bib44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.metabol.2016.10.008","article-title":"Metabolic amplification of insulin secretion is differentially desensitized by depolarization in the absence of exogenous fuels","volume":"67","author":"Schulze","year":"2017","journal-title":"Metabolism"},{"key":"2025091915585318000_bib45","doi-asserted-by":"crossref","first-page":"1395028","DOI":"10.3389\/fendo.2024.1395028","article-title":"Hypertonicity during a rapid rise in D-glucose mediates first-phase insulin secretion","volume":"15","author":"Kamat","year":"2024","journal-title":"Front Endocrinol"},{"issue":"5","key":"2025091915585318000_bib46","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1023\/A:1005621125812","article-title":"The oligomycin axis of mitochondrial ATP synthase: OSCP and the proton channel","volume":"32","author":"Devenish","year":"2000","journal-title":"J Bioenerg Biomembr"},{"issue":"1","key":"2025091915585318000_bib47","doi-asserted-by":"crossref","first-page":"C67","DOI":"10.1152\/ajpcell.1994.267.1.C67","article-title":"ATP depletion rather than mitochondrial depolarization mediates hepatocyte killing after metabolic inhibition","volume":"267","author":"Nieminen","year":"1994","journal-title":"Am J Physiol Cell Physiol"},{"issue":"8","key":"2025091915585318000_bib48","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1530\/EC-20-0289","article-title":"Fresh and cultured mouse islets differ in their response to nutrient stimulation","volume":"9","author":"Morsi","year":"2020","journal-title":"Endocr Connect"},{"issue":"8","key":"2025091915585318000_bib49","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.3390\/biomedicines12081747","article-title":"The proton leak of the inner mitochondrial membrane is enlarged in freshly isolated pancreatic islets","volume":"12","author":"Alshafei","year":"2024","journal-title":"Biomedicines"},{"issue":"3","key":"2025091915585318000_bib50","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1530\/JME-17-0063","article-title":"Glucose but not KCl diminishes submembrane granule turnover in mouse beta-cells","volume":"59","author":"Br\u00fcning","year":"2017","journal-title":"J Mol Endocrinol"},{"issue":"3","key":"2025091915585318000_bib51","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1113\/jphysiol.1976.sp011250","article-title":"Effects of glucose on 45Ca2+ uptake by pancreatic islets as studied with the lanthanum method","volume":"254","author":"Hellman","year":"1976","journal-title":"J Physiol"},{"issue":"2","key":"2025091915585318000_bib52","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1042\/bj1900361","article-title":"Calcium and pancreatic beta-cell function. The mechanism of insulin secretion studied with the aid of lanthanum","volume":"190","author":"Flatt","year":"1980","journal-title":"Biochem J"},{"issue":"4","key":"2025091915585318000_bib53","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1210\/endo.131.4.1327724","article-title":"Calcium channels in rat melanotrophs are permeable to manganese, cobalt, cadmium, and lanthanum, but not to nickel: evidence provided by fluorescence changes in fura-2-loaded cells","volume":"131","author":"Shibuya","year":"1992","journal-title":"Endocrinology"},{"issue":"2","key":"2025091915585318000_bib54","doi-asserted-by":"crossref","first-page":"bqz028","DOI":"10.1210\/endocr\/bqz028","article-title":"The endoplasmic reticulum and calcium homeostasis in pancreatic beta cells","volume":"161","author":"Zhang","year":"2020","journal-title":"Endocrinology"},{"issue":"40","key":"2025091915585318000_bib55","doi-asserted-by":"crossref","first-page":"30634","DOI":"10.1074\/jbc.M110.116681","article-title":"Plasma membrane Ca2+-ATPase overexpression depletes both mitochondrial and endoplasmic reticulum Ca2+ stores and triggers apoptosis in insulin-secreting BRIN-BD11 cells","volume":"285","author":"Jiang","year":"2010","journal-title":"J Biol Chem"},{"issue":"2","key":"2025091915585318000_bib56","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0014-5793(89)81572-8","article-title":"The dependence on intracellular ATP concentration of ATP-sensitive K-channels and of Na,K-ATPase in intact HIT-T15 beta-cells","volume":"257","author":"Niki","year":"1989","journal-title":"FEBS Lett"},{"issue":"6","key":"2025091915585318000_bib57","doi-asserted-by":"crossref","first-page":"856","DOI":"10.2337\/dbi23-0032","article-title":"Glucose regulation of \u03b2-Cell KATP channels: it is time for a new model","volume":"73","author":"Merrins","year":"2024","journal-title":"Diabetes"},{"issue":"6","key":"2025091915585318000_bib58","doi-asserted-by":"crossref","first-page":"849","DOI":"10.2337\/dbi23-0031","article-title":"Glucose regulation of \u03b2-Cell KATP channels: is a new model needed?","volume":"73","author":"Rutter","year":"2024","journal-title":"Diabetes"},{"issue":"1","key":"2025091915585318000_bib59","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1042\/bj2040161","article-title":"Proton-translocating Mg2+-dependent ATPase activity in insulin-secretory granules","volume":"204","author":"Hutton","year":"1982","journal-title":"Biochem J"},{"issue":"6","key":"2025091915585318000_bib60","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1083\/jcb.103.6.2273","article-title":"Conversion of proinsulin to insulin occurs coordinately with acidification of maturing secretory vesicles","volume":"103","author":"Orci","year":"1986","journal-title":"J Cell Biol"},{"issue":"2","key":"2025091915585318000_bib61","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1111\/j.1469-7793.1997.399bh.x","article-title":"Rapid ATP-dependent priming of secretory granules precedes Ca (2+)-induced exocytosis in mouse pancreatic B-cells","volume":"503","author":"Eliasson","year":"1997","journal-title":"J Physiol"},{"issue":"11","key":"2025091915585318000_bib62","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1242\/jcs.114.11.2145","article-title":"Priming of insulin granules for exocytosis by granular Cl(-) uptake and acidification","volume":"114","author":"Barg","year":"2001","journal-title":"J Cell Sci"},{"issue":"2","key":"2025091915585318000_bib63","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1083\/jcb.201303104","article-title":"The V-ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery","volume":"203","author":"Po\u00eba-Guyon","year":"2013","journal-title":"J Cell Biol"},{"issue":"23","key":"2025091915585318000_bib64","doi-asserted-by":"crossref","first-page":"4987","DOI":"10.1242\/jcs.158550","article-title":"The vacuolar-type H\u207a-ATPase at a glance\u2014more than a proton pump","volume":"127","author":"Maxson","year":"2014","journal-title":"J Cell Sci"},{"issue":"10","key":"2025091915585318000_bib65","doi-asserted-by":"crossref","first-page":"bqac125","DOI":"10.1210\/endocr\/bqac125","article-title":"Metabolic regulation of hormone secretion in beta-cells and alpha-cells of female mice: fundamental differences","volume":"163","author":"Br\u00fcning","year":"2022","journal-title":"Endocrinology"}],"container-title":["Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/full\/10.1093\/function\/zqaf033","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1093\/function\/zqaf033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:07:23Z","timestamp":1781629643000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/full\/10.1093\/function\/zqaf033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":65,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9,15]]}},"URL":"https:\/\/doi.org\/10.1093\/function\/zqaf033","relation":{},"ISSN":["2633-8823"],"issn-type":[{"value":"2633-8823","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025]]},"published":{"date-parts":[[2025]]},"article-number":"zqaf033"}}