{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T19:08:13Z","timestamp":1793732893365,"version":"4.6.1"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pacific Biosciences (PacBio) SMRT"},{"name":"Interagency Grizzly Bear Committee, United States Department of Agriculture National Institute of Food and Agriculture","award":["1018967"],"award-info":[{"award-number":["1018967"]}]},{"DOI":"10.13039\/100014252","name":"International Association for Bear Research and Management","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014252","id-type":"DOI","asserted-by":"publisher"}]},{"name":"T. N. Tollefson and Mazuri Exotic Animal Nutrition"},{"name":"Raili Korkka Brown Bear Endowment"},{"name":"Nutritional Ecology Endowment, and Bear Research and Conservation Endowment"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Understanding hibernation in brown bears (Ursus arctos) can provide insight into some human diseases. During hibernation, brown bears experience periods of insulin resistance, physical inactivity, extreme bradycardia, obesity, and the absence of urine production. These states closely mimic aspects of human diseases such as type 2 diabetes, muscle atrophy, as well as renal and heart failure. The reversibility of these states from hibernation to active season enables the identification of mediators with possible therapeutic value for humans. Recent studies have identified genes and pathways that are differentially expressed between active and hibernation seasons in bears. However, little is known about the role of differential expression of gene isoforms on hibernation physiology. To identify both distinct and novel mRNA isoforms, full-length RNA-sequencing (Iso-Seq) was performed on adipose, skeletal muscle, and liver from three individual bears sampled during both active and hibernation seasons. The existing reference genome annotation was improved by combining it with the Iso-Seq data. Short-read RNA-sequencing data from six individuals were mapped to the new reference annotation to quantify differential isoform usage (DIU) between tissues and seasons. We identified differentially expressed isoforms in all three tissues, to varying degrees. Adipose had a high level of DIU with isoform switching, regardless of whether the genes were differentially expressed. Our analyses revealed that DIU, even in the absence of differential gene expression, is an important mechanism for modulating genes during hibernation. These findings demonstrate the value of isoform expression studies and will serve as the basis for deeper exploration into hibernation biology.<\/jats:p>","DOI":"10.1093\/g3journal\/jkab422","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T07:22:45Z","timestamp":1640071365000},"source":"Crossref","is-referenced-by-count":18,"title":["Long-read isoform sequencing reveals tissue-specific isoform expression between active and hibernating brown bears (\n                    <i>Ursus arctos<\/i>\n                    )"],"prefix":"10.1093","volume":"12","author":[{"given":"Elizabeth","family":"Tseng","sequence":"first","affiliation":[{"name":"Pacific Biosciences, Menlo Park, CA 94025, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason G","family":"Underwood","sequence":"additional","affiliation":[{"name":"Pacific Biosciences, Menlo Park, CA 94025, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon 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