{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T20:45:40Z","timestamp":1784234740803,"version":"3.55.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Caenorhabditis Genetic Center"},{"name":"NIH Office of Research Infrastructure Programs","award":["P40 OD010440"],"award-info":[{"award-number":["P40 OD010440"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM103860"],"award-info":[{"award-number":["GM103860"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM103860S1"],"award-info":[{"award-number":["GM103860S1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Biological sex affects numerous aspects of biology, yet how sex influences different biological processes have not been extensively studied at the molecular level. Caenorhabditis elegans, with both hermaphrodites (functionally females as adults) and males, is an excellent system to uncover how sex influences physiology. Here, we describe a method to isolate large quantities of C. elegans males by conditionally degrading DPY-27, a component of the dosage compensation complex essential for hermaphrodite, but not male, development. We show that germ cells from males isolated following DPY-27 degradation undergo meiosis and spermiogenesis like wild type and these males are competent to mate and sire viable offspring. We further demonstrate the efficacy of this system by analyzing gene expression and performing affinity pull-downs from male worm extracts.<\/jats:p>","DOI":"10.1093\/g3journal\/jkac085","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T08:43:14Z","timestamp":1649666594000},"source":"Crossref","is-referenced-by-count":8,"title":["Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of\n                    <i>Caenorhabditis elegans<\/i>\n                    males for molecular and biochemical analyses"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4865-0101","authenticated-orcid":false,"given":"Qianyan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA"},{"name":"Biochemistry, Molecular, Cellular and Developmental Biology Graduate Group, University of California, Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9846-0675","authenticated-orcid":false,"given":"Arshdeep","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1572-6181","authenticated-orcid":false,"given":"Benjamin","family":"Mallory","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4693-2359","authenticated-orcid":false,"given":"Sara","family":"Hariri","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA"},{"name":"Biochemistry, Molecular, Cellular and Developmental Biology Graduate Group, University of California, Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2733-7506","authenticated-orcid":false,"given":"JoAnne","family":"Engebrecht","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA"},{"name":"Biochemistry, Molecular, Cellular and Developmental Biology Graduate Group, University of California, Davis, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"issue":"3","key":"2022050609281514100_jkac085-B1","doi-asserted-by":"crossref","first-page":"iyab006","DOI":"10.1093\/genetics\/iyab006","article-title":"An expanded auxin-inducible degron toolkit for Caenorhabditis 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