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Previous work led to the identification of 9 srf or bus genes; here, we report molecular identification and characterization of a further 10 surface-affecting genes. Three of these were found to encode factors implicated in glycosylation (srf-2, bus-5, and bus-22), like several of those previously reported; srf-2 belongs to the GT92 family of putative galactosyltransferases, and bus-5 is homologous to human dTDP-D-glucose 4,6-dehydratase, which is implicated in Catel\u2013Manzke syndrome. Other genes encoded proteins with sequence similarity to phosphatidylinositol phosphatases (bus-6), Patched-related receptors (ptr-15\/bus-13), steroid dehydrogenases (dhs-5\/bus-21), or glypiation factors (bus-24). Three genes appeared to be nematode-specific (srf-5, bus-10, and bus-28). Many mutants exhibited cuticle fragility as revealed by bleach and detergent sensitivity; this fragility was correlated with increased drug sensitivity, as well as with abnormal skiddy locomotion. Most of the genes examined were found to be expressed in epidermal seam cells, which appear to be important for synthesizing nematode surface coat. The results reveal the genetic and biochemical complexity of this critical surface layer, and provide new tools for its analysis.<\/jats:p>","DOI":"10.1093\/g3journal\/jkad056","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T15:46:35Z","timestamp":1678722395000},"source":"Crossref","is-referenced-by-count":11,"title":["Isolation and molecular identification of nematode surface mutants with resistance to bacterial pathogens"],"prefix":"10.1093","volume":"13","author":[{"given":"Delia","family":"O\u2019Rourke","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Oxford , Oxford OX1 3QU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J","family":"Gravato-Nobre","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford , Oxford OX1 3QU , 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