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A frequently chosen tool for global disruption of phase separation via weak hydrophobic interactions is treatment with 1,6-hexanediol. This study evaluates the cytotoxic and genotoxic effects of treating live fission yeast with 1,6-hexanediol. We find that 1,6-hexanediol causes a drastic decrease in cell survival and growth rate. We also see a reduction in HP1 protein foci and increase in DNA damage foci. However, there is no evidence for increased genomic instability in two classically phase-separated domains, the heterochromatic pericentromere and the nucleolar rDNA repeats. This study reveals that 1,6-hexanediol is a blunt tool for phase separation inhibition and its secondary effects must be taken into consideration during its in vivo use.<\/jats:p>","DOI":"10.1093\/g3journal\/jkad123","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T12:15:32Z","timestamp":1687263332000},"source":"Crossref","is-referenced-by-count":5,"title":["Impact of 1,6-hexanediol on <i>Schizosaccharomyces pombe<\/i> genome stability"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-6028","authenticated-orcid":false,"given":"Chance E","family":"Jones","sequence":"first","affiliation":[{"name":"Section of Molecular & Computational Biology, University of Southern California , Los Angeles, CA 90089 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4895-8598","authenticated-orcid":false,"given":"Susan 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