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Its multifaceted roles in cellular protein homeostasis as a nucleotide exchange factor (NEF), as a protein-disaggregase and as a chaperone linked to protein synthesis (CLIPS) are well documented. In the current study, we show that SSE1 genetically interacts with IRE1 and HAC1, the endoplasmic reticulum-unfolded protein response (ER-UPR) sensors implicating its role in ER protein homeostasis. Interestingly, the absence of this chaperone imparts unusual resistance to tunicamycin-induced ER stress which depends on the intact Ire1-Hac1 mediated ER-UPR signaling. Furthermore, cells lacking SSE1 show inefficient ER-stress-responsive reorganization of translating ribosomes from polysomes to monosomes that drive uninterrupted protein translation during tunicamycin stress. In consequence, the sse1\u0394 strain shows prominently faster reversal from ER-UPR activated state indicating quicker restoration of homeostasis, in comparison to the wild-type (WT) cells. Importantly, Sse1 plays a critical role in controlling the ER-stress-mediated cell division arrest, which is escaped in sse1\u0394 strain during chronic tunicamycin stress. Accordingly, sse1\u0394 strain shows significantly higher cell viability in comparison to WT yeast imparting the stark fitness following short-term as well as long-term tunicamycin stress. These data, all together, suggest that cytosolic chaperone Sse1 is an important modulator of ER stress response in yeast and it controls stress-induced cell division arrest and cell death during overwhelming ER stress induced by tunicamycin.<\/jats:p>","DOI":"10.1093\/g3journal\/jkae075","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T06:36:26Z","timestamp":1712298986000},"source":"Crossref","is-referenced-by-count":5,"title":["Sse1, Hsp110 chaperone of yeast, controls the cellular fate during endoplasmic reticulum stress"],"prefix":"10.1093","volume":"14","author":[{"given":"Mainak Pratim","family":"Jha","sequence":"first","affiliation":[{"name":"Protein Homeostasis Laboratory, Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institution of Eminence , Delhi-NCR, Gautam Buddha Nagar, Greater Noida, Uttar Pradesh 201314 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4647-6616","authenticated-orcid":false,"given":"Vignesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Chemical and Systems Biology Unit, CSIR\u2013Institute of Genomics and Integrative Biology , New Delhi 110025 , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002, Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmita","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Chemical and Systems Biology Unit, CSIR\u2013Institute of Genomics and Integrative Biology , New Delhi 110025 , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002, Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0080-9243","authenticated-orcid":false,"given":"Koyeli","family":"Mapa","sequence":"additional","affiliation":[{"name":"Protein Homeostasis Laboratory, Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institution of Eminence , Delhi-NCR, Gautam Buddha Nagar, Greater Noida, Uttar Pradesh 201314 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,5]]},"reference":[{"issue":"1","key":"2024060514121627300_jkae075-B1","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.cell.2005.11.039","article-title":"Systems analyses reveal two chaperone networks with distinct functions in eukaryotic cells","volume":"124","author":"Albanese","year":"2006","journal-title":"Cell"},{"issue":"43","key":"2024060514121627300_jkae075-B2","doi-asserted-by":"publisher","first-page":"16519","DOI":"10.1073\/pnas.0804187105","article-title":". 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