{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:26:26Z","timestamp":1787768786018,"version":"build-2784847793"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01AG073238"],"award-info":[{"award-number":["R01AG073238"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>During mammalian reproduction, sperm are delivered to the female reproductive tract bathed in a complex medium known as seminal fluid, which plays key roles in signaling to the female reproductive tract and in nourishing sperm for their onwards journey. Along with minor contributions from the prostate and the epididymis, the majority of seminal fluid is produced by a somewhat understudied organ known as the seminal vesicle. Here, we report the first single-cell RNA-seq atlas of the mouse seminal vesicle, generated using tissues obtained from 23 mice of varying ages, exposed to a range of dietary challenges. We define the transcriptome of the secretory cells in this tissue, identifying a relatively homogeneous population of the epithelial cells which are responsible for producing the majority of seminal fluid. We also define the immune cell populations\u2014including large populations of macrophages, dendritic cells, T cells, and NKT cells\u2014which have the potential to play roles in producing the various immune mediators present in seminal plasma. Together, our data provide a resource for understanding the composition of an understudied reproductive tissue, with potential implications for paternal control of offspring development and metabolism.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf045","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T17:12:25Z","timestamp":1741108345000},"source":"Crossref","is-referenced-by-count":1,"title":["A single cell atlas of the mouse seminal vesicle"],"prefix":"10.1093","volume":"15","author":[{"given":"Fengyun","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Desevin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6252-3075","authenticated-orcid":false,"given":"Yu","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanmathi","family":"Parameswaran","sequence":"additional","affiliation":[{"name":"Hunter Medical Research Institute Infertility and Reproduction Research Program, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle , Callaghan, NSW 2305 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jemma","family":"Mayall","sequence":"additional","affiliation":[{"name":"Hunter Medical Research Institute, The University of Newcastle, Newcastle Immune Health Program, , NSW 2305 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0051-1754","authenticated-orcid":false,"given":"Vera","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Krietenstein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Art\u00fcr","family":"Manukyan","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Integrative Biology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiangzong","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Galan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Hsiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia V","family":"Shindyapina","sequence":"additional","affiliation":[{"name":"Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Harvard University , Boston, MA 02115 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim N","family":"Gladyshev","sequence":"additional","affiliation":[{"name":"Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Harvard University , Boston, MA 02115 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Garber","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Integrative Biology, University of Massachusetts Chan Medical School, University of Massachusetts , Worcester, MA 01605 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