{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:22:03Z","timestamp":1788826923510,"version":"build-2803163510"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-20-CE20-0015-01"],"award-info":[{"award-number":["ANR-20-CE20-0015-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The uses of Cannabis sativa, a dioecious species with an XY sex chromosome system, are varying from fiber and oil to cannabinoids, among others. In most cases, males are undesirable, and the sexual dimorphism at immature plants is too subtle for reliable phenotypic sexing, making genetic approaches promising. In this technical note, we present a multiplex PCR mix that includes 2 markers of Y-specific coding regions and 1 autosomal control marker. This PCR mix, tested across 12 hemp-type cultivars, encompassing approximately 200 individuals, achieved a 99.5% true positive rate in identifying the sex of C. sativa samples. Despite being tested on a limited number of germplasm, we believe that these markers offer a promising solution for sexing a larger diversity of C. sativa seedlings.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf077","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T19:13:17Z","timestamp":1744225997000},"source":"Crossref","is-referenced-by-count":5,"title":["Promising high fidelity genetic markers for sexing <i>Cannabis sativa<\/i> seedlings"],"prefix":"10.1093","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3088-3954","authenticated-orcid":false,"given":"Djivan","family":"Prentout","sequence":"first","affiliation":[{"name":"Laboratoire de Biom\u00e9trie et Biologie \u00c9volutive UMR 5558, Universit\u00e9 de Lyon, Universit\u00e9 Lyon 1, CNRS , Villeurbanne F-69622 ,","place":["France"]},{"name":"Department of Biological Sciences, Columbia University , New York, NY 10027 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma","family":"El Aoudati","sequence":"additional","affiliation":[{"name":"Laboratoire de Biom\u00e9trie et Biologie \u00c9volutive UMR 5558, Universit\u00e9 de Lyon, Universit\u00e9 Lyon 1, CNRS , Villeurbanne F-69622 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Mathis","sequence":"additional","affiliation":[{"name":"Hemp-it ADN , R&D Department, Beaufort en Anjou 49 250 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel A B","family":"Marais","sequence":"additional","affiliation":[{"name":"Laboratoire de Biom\u00e9trie et Biologie \u00c9volutive UMR 5558, Universit\u00e9 de Lyon, Universit\u00e9 Lyon 1, CNRS , Villeurbanne F-69622 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Henri","sequence":"additional","affiliation":[{"name":"Laboratoire de Biom\u00e9trie et Biologie \u00c9volutive UMR 5558, Universit\u00e9 de Lyon, Universit\u00e9 Lyon 1, CNRS , Villeurbanne F-69622 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,9]]},"reference":[{"key":"2025060406574379200_jkaf077-B1","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-54564-6","volume-title":"Cannabis sativa L.\u2014Botany and Biotechnology","author":"Chandra","year":"2017"},{"issue":"1","key":"2025060406574379200_jkaf077-B2","doi-asserted-by":"publisher","first-page":"jkad216","DOI":"10.1093\/g3journal\/jkad216","article-title":"An affordable and convenient diagnostic marker to identify male and female hop plants","volume":"14","author":"Clare","year":"2024","journal-title":"G3 (Bethesda)"},{"issue":"1","key":"2025060406574379200_jkaf077-B3","doi-asserted-by":"publisher","first-page":"e85118","DOI":"10.1371\/journal.pone.0085118","article-title":"Molecular cytogenetic characterization of the dioecious Cannabis sativa with an XY chromosome sex determination system","volume":"9","author":"Divashuk","year":"2014","journal-title":"PLoS One"},{"key":"2025060406574379200_jkaf077-B4","doi-asserted-by":"publisher","first-page":"113194","DOI":"10.1016\/j.indcrop.2020.113194","article-title":"Extraction of high molecular weight DNA suitable for next-generation sequencing from the fiber crop abaca","volume":"161","author":"Koh","year":"2021","journal-title":"Ind Crops Prod"},{"issue":"4","key":"2025060406574379200_jkaf077-B5","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1093\/bioinformatics\/btt703","article-title":"Causal analysis approaches in ingenuity pathway analysis","volume":"30","author":"Kr\u00e4mer","year":"2014","journal-title":"Bioinforma Oxf Engl"},{"issue":"VII","key":"2025060406574379200_jkaf077-B6","doi-asserted-by":"publisher","first-page":"82","DOI":"10.51584\/IJRIAS.2023.8709","article-title":"Cannabis sativa: dioecious into monoecious plants influencing sex determination","volume":"VIII","author":"Malabadi","year":"2023","journal-title":"Int J Res Innov Appl Sci"},{"issue":"2","key":"2025060406574379200_jkaf077-B7","doi-asserted-by":"publisher","first-page":"170","DOI":"10.30574\/wjbphs.2024.17.2.0044","article-title":"Cannabis sativa: a therapeutic medicinal plant-global marketing updates","volume":"17","author":"Malabadi","year":"2024","journal-title":"World J Biol Pharm Health Sci"},{"issue":"1","key":"2025060406574379200_jkaf077-B8","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1146\/annurev-arplant-042110-103914","article-title":"Sex chromosomes in land plants","volume":"62","author":"Ming","year":"2011","journal-title":"Annu Rev Plant Biol"},{"issue":"2","key":"2025060406574379200_jkaf077-B9","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1101\/gr.251207.119","article-title":"An efficient RNA-seq-based segregation analysis identifies the sex chromosomes of Cannabis sativa","volume":"30","author":"Prentout","year":"2020","journal-title":"Genome Res"},{"issue":"4","key":"2025060406574379200_jkaf077-B10","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1111\/nph.17456","article-title":"Plant genera Cannabis and Humulus share the same pair of well-differentiated sex chromosomes","volume":"231","author":"Prentout","year":"2021","journal-title":"New Phytol"},{"issue":"38","key":"2025060406574379200_jkaf077-B11","doi-asserted-by":"publisher","first-page":"52832","DOI":"10.1007\/s11356-021-16264-5","article-title":"Evaluation of hemp (Cannabis sativa L.) as an industrial crop: a review","volume":"28","author":"Rehman","year":"2021","journal-title":"Environ Sci Pollut Res"},{"issue":"29","key":"2025060406574379200_jkaf077-B12","doi-asserted-by":"publisher","first-page":"eabg2286","DOI":"10.1126\/sciadv.abg2286","article-title":"Large-scale whole-genome resequencing unravels the domestication history of Cannabis sativa","volume":"7","author":"Ren","year":"2021","journal-title":"Sci Adv"},{"issue":"5","key":"2025060406574379200_jkaf077-B13","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1139\/g05-056","article-title":"RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L","volume":"48","author":"Sakamoto","year":"2005","journal-title":"Genome"},{"issue":"D1","key":"2025060406574379200_jkaf077-B14","doi-asserted-by":"publisher","first-page":"D20","DOI":"10.1093\/nar\/gkab1112","article-title":"Database resources of the national center for biotechnology information","volume":"50","author":"Sayers","year":"2021","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"2025060406574379200_jkaf077-B15","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1023\/A:1020204729122","article-title":"Novel male-specific molecular markers (MADC5, MADC6) in hemp","volume":"127","author":"T\u00f6rj\u00e9k","year":"2002","journal-title":"Euphytica"},{"issue":"3","key":"2025060406574379200_jkaf077-B16","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/gcbb.12667","article-title":"Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L","volume":"12","author":"Toth","year":"2020","journal-title":"Glob Change Biol Bioenergy."},{"issue":"7","key":"2025060406574379200_jkaf077-B17","doi-asserted-by":"publisher","first-page":"1492","DOI":"10.3390\/agronomy12071492","article-title":"Cannabis sativa L.: crop management and abiotic factors that affect phytocannabinoid production","volume":"12","author":"Trancoso","year":"2022","journal-title":"Agronomy"},{"issue":"10","key":"2025060406574379200_jkaf077-B18","doi-asserted-by":"publisher","first-page":"R102","DOI":"10.1186\/gb-2011-12-10-r102","article-title":"The draft genome and transcriptome of Cannabis sativa","volume":"12","author":"van Bakel","year":"2011","journal-title":"Genome Biol"}],"container-title":["G3: Genes, Genomes, Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/g3journal\/advance-article-pdf\/doi\/10.1093\/g3journal\/jkaf077\/62899452\/jkaf077.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/g3journal\/article-pdf\/15\/6\/jkaf077\/62899452\/jkaf077.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/g3journal\/article-pdf\/15\/6\/jkaf077\/62899452\/jkaf077.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T10:57:48Z","timestamp":1749034668000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/g3journal\/article\/doi\/10.1093\/g3journal\/jkaf077\/8109470"}},"subtitle":[],"editor":[{"given":"E","family":"Akhunov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,4,9]]},"references-count":18,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,4,9]]},"published-print":{"date-parts":[[2025,6,4]]}},"URL":"https:\/\/doi.org\/10.1093\/g3journal\/jkaf077","relation":{},"ISSN":["2160-1836"],"issn-type":[{"value":"2160-1836","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,6]]},"published":{"date-parts":[[2025,4,9]]},"article-number":"jkaf077"}}