{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:36:52Z","timestamp":1788345412965,"version":"build-2803163510"},"reference-count":67,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,8,17]],"date-time":"2025-08-17T00:00:00Z","timestamp":1755388800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001543","name":"Neurological Foundation of New Zealand","doi-asserted-by":"publisher","award":["PRG1732"],"award-info":[{"award-number":["PRG1732"]}],"id":[{"id":"10.13039\/501100001543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>RECQL4 encodes a RecQ helicase, one of a family of DNA unwinding enzymes with roles in DNA replication, double-strand break repair, and genomic stability. Pathogenic variants in RECQL4 are clinically associated with 3 rare autosomal recessive conditions: Rothmund\u2013Thomson syndrome type II, Baller\u2013Gerold syndrome, and RAPADILINO syndrome. These 3 syndromes show overlapping growth retardation, low bone density, and skeletal defects affecting the arms and hands. Here, we take advantage of the ability to generate one-sided CRISPR knockdowns of recql4 in Xenopus laevis tadpoles. Tadpoles develop normally until feeding starts, after which growth slows on the edited side, leading to a curved posture, smaller eyes (microphthalmia), and reduced head size (microcephaly). Forelimb buds fail to develop, leading to complete absence of the forelimb on the edited side. Additionally, Meckel's cartilage (lower jaw) ossification is absent or reduced and the hyoid cartilage is smaller, but this is not due to deficiencies in cranial neural crest migration on the edited side. Knockdown of recql4 also results in hypoplastic vasculature, with reduced branching from the aorta on the edited side. Taken together, our results clearly show the utility of unilateral CRISPR editing in Xenopus for understanding the specific phenotypic developmental effects of mutations affecting cell proliferation.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf179","type":"journal-article","created":{"date-parts":[[2025,8,17]],"date-time":"2025-08-17T12:38:00Z","timestamp":1755434280000},"source":"Crossref","is-referenced-by-count":1,"title":["Unilateral loss of\n                    <i>recql4<\/i>\n                    function in\n                    <i>Xenopus laevis<\/i>\n                    tadpoles leads to ipsilateral ablation of the forelimb, hypoplastic Meckel's cartilage, and vascular defects"],"prefix":"10.1093","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6489-7425","authenticated-orcid":false,"given":"Caroline W","family":"Beck","sequence":"first","affiliation":[{"name":"Department of Zoology, University of Otago , Dunedin 9016, Otago ,","place":["New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Reily-Bell","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of Otago , Dunedin 9016, Otago ,","place":["New Zealand"]},{"name":"Department of Biochemistry, University of Otago , Dunedin 9016, Otago ,","place":["New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise S","family":"Bicknell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Otago , Dunedin 9016, Otago ,","place":["New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,16]]},"reference":[{"key":"2025100811220735100_jkaf179-B1","doi-asserted-by":"publisher","first-page":"e147301","DOI":"10.1172\/JCI147301","article-title":"RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase 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