{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:49:04Z","timestamp":1784875744799,"version":"3.55.0"},"reference-count":108,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["206194"],"award-info":[{"award-number":["206194"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["218328"],"award-info":[{"award-number":["218328"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Many, but not all, parasitic filarial nematodes (Onchocercidae) carry intracellular, maternally transmitted, alphaproteobacterial Wolbachia symbionts. The association between filarial nematodes and Wolbachia is often portrayed as mutualist, where the nematode is reliant on Wolbachia for an essential but unknown service. Wolbachia are targets for antifilarial chemotherapeutic interventions for human disease. Wolbachia of Onchocercidae derive from four of the major supergroups (C, D, F, and J) defined within the genus. We explored the evolutionary history of the filarial nematode-Wolbachia symbiosis in 22 nematode species, 16 of which have current Wolbachia infections, by screening the nematode nuclear genome sequences for nuclear Wolbachia transfers, fragments of the Wolbachia genome that have been inserted into the nuclear genome. We identified Wolbachia insertions in 5 of the 6 species that have no current Wolbachia infection, showing they have previously had and have now lost Wolbachia infections. In currently infected species, we found a diversity of origins of the insertions, including many cases where they derived from a different supergroup to the current live infection. Mapping the origins of the insertions onto the filarial nematode phylogeny we derive a complex model of evolution of Wolbachia symbiosis. The history of association between Wolbachia and onchocercid nematodes includes not only cospeciation, as would be expected from a mutualist symbiosis, but also loss (in the 5 Wolbachia-free species), frequent symbiont replacement, and dual infection. This dynamic pattern is challenging to models that assume host\u2013symbiont mutualism.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf226","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T08:13:44Z","timestamp":1759306424000},"source":"Crossref","is-referenced-by-count":2,"title":["Ghosts of symbionts past: the hidden history of the dynamic association between filarial nematodes and their\n                    <i>Wolbachia<\/i>\n                    endosymbionts"],"prefix":"10.1093","volume":"15","author":[{"given":"Emmelien","family":"Vancaester","sequence":"first","affiliation":[{"name":"Tree of Life, Wellcome Sanger Institute , Hinxton CB10 1SA ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1428-0608","authenticated-orcid":false,"given":"Guy R","family":"Oldrieve","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Edinburgh , Edinburgh EH9 3TF ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Reid","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Edinburgh , Edinburgh EH9 3TF ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3406-3715","authenticated-orcid":false,"given":"Georgios","family":"Koutsovoulos","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Edinburgh , Edinburgh EH9 3TF ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7887-0186","authenticated-orcid":false,"given":"Dominik R","family":"Laetsch","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Edinburgh , Edinburgh EH9 3TF ,","place":["United 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,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2842-8100","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Kr\u00fccken","sequence":"additional","affiliation":[{"name":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin , Berlin 14163 ,","place":["Germany"]},{"name":"Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin , Berlin 14163 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7989-3929","authenticated-orcid":false,"given":"Adrian","family":"Wolstenholme","sequence":"additional","affiliation":[{"name":"Dept of Infectious Diseases, University of Georgia , 501 D W Brooks Drive, Athens, GA 30602 ,","place":["United 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