{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:31:49Z","timestamp":1793345509805,"version":"4.5.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT-159702 (HDL)"],"award-info":[{"award-number":["PJT-159702 (HDL)"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT-190124 (HDL)"],"award-info":[{"award-number":["PJT-190124 (HDL)"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council","doi-asserted-by":"publisher","award":["RGPIN-2022-05163 (JAB)"],"award-info":[{"award-number":["RGPIN-2022-05163 (JAB)"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Canada Graduate Scholarship"},{"name":"University of Toronto Open Fellowship"},{"DOI":"10.13039\/100030650","name":"Department of Molecular Genetics Eric Hani Fellowship","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030650","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Toronto Open Fellowship"},{"name":"NSERC Canada Graduate Scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>ORB2 is the Drosophila ortholog of the human CPEB2-4 family of RNA-binding proteins, which include a conserved C-terminal Zinc-binding (\u2018ZZ\u2019) domain. We have recently shown that this domain interacts with several translational co-repressors in the early embryo, and that deletion of this domain from the endogenous orb2 gene results in derepression of its target mRNAs. Here we assess the effect of deletion of the ZZ domain on spermatogenesis. We find that deletion of the ZZ domain does not affect spatial localization of the ORB2 protein; orb2\u0394ZZ flies are sterile and lack mature sperm; meiosis is mostly normal in orb2\u0394ZZ testes; individualization complexes are defective and spermatid individualization fails; proteins known to play a role in spermatid individualization\u2014ORB, IMP, SOTI\u2014are mislocalized; and the SOTI-dependent Cleaved Caspase-3 gradient no longer forms in orb2\u0394ZZ mutant testes.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf260","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T20:59:23Z","timestamp":1761944363000},"source":"Crossref","is-referenced-by-count":4,"title":["The C-terminal ZZ domain of the Drosophila ORB2 RNA-binding protein is required for spermatid individualization"],"prefix":"10.1093","volume":"16","author":[{"given":"Timothy C H","family":"Low","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto, 661 University Avenue , Toronto Ontario M5G 1M1 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2324-1862","authenticated-orcid":false,"given":"Brook L","family":"Falk","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto, 661 University Avenue , Toronto Ontario M5G 1M1 ,","place":["Canada"]},{"name":"Program in Cell & Systems Biology, Research Institute, the Hospital for Sick Children , 686 Bay Street, Toronto, Ontario M5G 0A4 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5925-9901","authenticated-orcid":false,"given":"Julie A","family":"Brill","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto, 661 University Avenue , Toronto Ontario M5G 1M1 ,","place":["Canada"]},{"name":"Program in Cell & Systems Biology, Research Institute, the Hospital for Sick Children , 686 Bay Street, Toronto, Ontario M5G 0A4 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7372-4419","authenticated-orcid":false,"given":"Howard D","family":"Lipshitz","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto, 661 University Avenue , Toronto Ontario M5G 1M1 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"2026010806013846900_jkaf260-B1","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1242\/dev.021949","article-title":"Post-meiotic transcription in Drosophila testes","volume":"135","author":"Barreau","year":"2008","journal-title":"Development"},{"key":"2026010806013846900_jkaf260-B2","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1083\/jcb.201106152","article-title":"DNA damage-inducible 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