{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T22:08:13Z","timestamp":1770242893086,"version":"3.49.0"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The anchor cell (AC) of the Caenorhabditis elegans hermaphrodite somatic gonad primordium is a signaling nexus that regulates uterine and vulval development. As the somatic gonad primordium is forming, four cells, two \u03b1 and two \u03b2 cells, are born with the potential to be the AC. This potential becomes restricted to the \u03b1 cells, which undergo the LIN-12\/Notch-mediated AC\/VU decision to resolve which \u03b1 cell will become the AC. The transcription factor HLH-2, the sole E\/Daughterless protein ortholog in C. elegans, is critical for this process, and dynamic regulation of hlh-2 transcription contributes to the robust specification of a single AC. The hlh-2prox regulatory element mediates the dynamic pattern of hlh-2 transcription: the initial expression in the parents of the \u03b1 and \u03b2 cells, which is briefly sustained in the \u03b1 and \u03b2 cells after they are born; its subsequent restriction to the \u03b1 cells during the AC\/VU decision; and its continued expression in the AC. In this study, we identify the cis-acting sequences within hlh-2prox and transcription factors required for the initial expression of hlh-2 in the \u03b1 and \u03b2 cells and their parents, demonstrate that the Wnt\/\u03b2-catenin Asymmetry Pathway (W\u03b2A) regulates restriction of hlh-2 transcription to the \u03b1 cells, and show that the maintenance of hlh-2 transcription in \u03b1 cells requires distinct elements and the chromatin factor LSY-12. This analysis extends our understanding of regulatory mechanisms that operate during a precise and robust Notch-mediated lateral specification paradigm.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf288","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:20:33Z","timestamp":1764699633000},"source":"Crossref","is-referenced-by-count":0,"title":["Regulation of\n                    <i>hlh-2<\/i>\n                    transcription during specification of the anchor cell of the\n                    <i>C. elegans<\/i>\n                    hermaphrodite gonad"],"prefix":"10.1093","volume":"16","author":[{"given":"Jee Hun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Columbia University , 1212 Amsterdam Avenue, New York, NY 10027 ,","place":["United 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