{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T22:34:58Z","timestamp":1770244498524,"version":"3.49.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012264","name":"NIH","doi-asserted-by":"publisher","award":["R35GM145378"],"award-info":[{"award-number":["R35GM145378"]}],"id":[{"id":"10.13039\/501100012264","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aminoacyl-tRNA synthetases (aaRSs) are essential for translation, as they charge tRNA molecules with their corresponding amino acids. Alterations in aaRSs can significantly disrupt both cytosolic and mitochondrial translation. Through a forward genetic screen for mitochondrial unfolded protein response (UPRmt) activators in Caenorhabditis elegans, we identified a missense mutation (P447V) in the previously uncharacterized gene Y105E8A.20, which encodes for a methionine tRNA synthetase (MetRS). Here, we characterize the UPRmt induction by Y105E8A.20, which we call mars-2, and demonstrate that the P447V allele is a loss-of-function mutation. Furthermore, we show that impaired mars-2 activity leads to reduced mitochondrial-encoded protein abundance, depletion of mitochondrial membrane potential, fragmented mitochondrial morphology, and mild developmental delay, although the animals remain viable. Hence, this hypomorphic mars-2(P447V) strain provides a valuable tool for studying mitochondrial translation and understanding how aaRSs are involved in mitochondrial homeostasis.<\/jats:p>","DOI":"10.1093\/g3journal\/jkaf298","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T18:05:06Z","timestamp":1765217106000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification of an uncharacterized gene as a mitochondrial methionine tRNA synthetase in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5269-2139","authenticated-orcid":false,"given":"Bharat Vivan","family":"Thapa","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Vanderbilt University , Nashville, TN 37232 ,","place":["United States"]},{"name":"Department of Metabolism and Nutritional Programming, Van Andel Institute , Grand Rapids, MI 49503 ,","place":["United 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