{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T21:01:43Z","timestamp":1775077303741,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH-NIGMS","award":["P20 GM103446"],"award-info":[{"award-number":["P20 GM103446"]}]},{"name":"NIH-NIGMS","award":["P20 GM139760"],"award-info":[{"award-number":["P20 GM139760"]}]},{"DOI":"10.13039\/100025212","name":"State of Delaware","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100025212","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Andor Dragonfly","award":["S10 OD030321"],"award-info":[{"award-number":["S10 OD030321"]}]},{"name":"Zeiss LSM980"},{"name":"NIH-NIGMS","award":["P20 GM103446"],"award-info":[{"award-number":["P20 GM103446"]}]},{"name":"NIH-NIGMS","award":["P20 GM113125"],"award-info":[{"award-number":["P20 GM113125"]}]},{"name":"Institutional Development Award"},{"DOI":"10.13039\/100000057","name":"National Institute of Health\u2019s National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103446"],"award-info":[{"award-number":["P20GM103446"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of Health\u2019s National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM142524"],"award-info":[{"award-number":["R35GM142524"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016958","name":"NIH Office of Research Infrastructure Programs","doi-asserted-by":"publisher","award":["P40 0D010440"],"award-info":[{"award-number":["P40 0D010440"]}],"id":[{"id":"10.13039\/100016958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Metazoan eggs are surrounded by a specialized coat of extracellular matrix that mediates sperm-egg interactions. This coat is rapidly remodeled after fertilization to form a barrier that prevents polyspermy, protects against environmental insults, and provides structural support to the developing embryo. In C. elegans, several oocyte surface proteins have been identified that mediate these events. However, whether two of these proteins, EGG-1 and EGG-2, are required for fertilization or downstream events has been unclear. Here, we address this question using more recent advances in genome editing tools through the creation of egg-1 \u00a0egg-2 deletions of the endogenous loci. We found that egg-1 \u00a0egg-2 oocytes are fertilization competent and form rudimentary eggshells. While the integrity of the egg-1 \u00a0egg-2 eggshell is compromised and often ruptures within the uterus, some embryos can undergo several rounds of cell division. Absence of EGG-1 and EGG-2 results in the mislocalization of proteins on the embryo surface and eggshell. CBD-1, CHS-1, and MBK-2, components of the egg activation complex and outermost eggshell layer, were mislocalized, while the localization of CPG-1, a component of an inner eggshell layer, was not perturbed. Overall, our findings demonstrate that EGG-1 and EGG-2 are not required for fertilization but rather are involved in the organization of eggshell structural components and oocyte plasma membrane proteins.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag013","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T20:20:10Z","timestamp":1768854010000},"source":"Crossref","is-referenced-by-count":0,"title":["Oocyte surface proteins EGG-1 and EGG-2 are required for eggshell integrity in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"16","author":[{"given":"Ji Kent","family":"Kwah","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Delaware , Newark, DE 19716 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4662-7144","authenticated-orcid":false,"given":"Shannon","family":"Pfeiffer","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Delaware , Newark, DE 19716 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mst Gitika","family":"Khanom","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Delaware , Newark, DE 19716 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7697-8580","authenticated-orcid":false,"given":"Aimee","family":"Jaramillo-Lambert","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Delaware , Newark, DE 19716 ,","place":["United 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