{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:08:17Z","timestamp":1780506497278,"version":"3.54.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2020-03300"],"award-info":[{"award-number":["2020-03300"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["19 0029"],"award-info":[{"award-number":["19 0029"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wilhelm och Martina Lundgrens Vetenskapsfond","award":["2025-GU-5010"],"award-info":[{"award-number":["2025-GU-5010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In C. elegans, the epidermis and its overlying extracellular matrix form a primary protective barrier, functioning as the first line of defense against environmental factors. To properly develop those cellular boundaries, a tightly controlled interaction of many molecules and pathways is needed. Mutant alleles of paqr-2 and iglr-2 (lipid homeostasis), dpy-21 (membrane trafficking), and sma-1 (actin-binding spectrin) result in hermaphrodite tail tip defects suggesting that this simple 4-cell structure can serve as a sensitive model for the identification of pathways responsible for the establishment of cellular boundaries. With this in mind, we performed a small forward genetics screen of \u223c800 ethyl methanesulfonate-mutagenized haploid genomes and identified 21 mutants with a Tail End Defects in the hermaphrodite phenotype. Whole genome sequencing of these mutants identified mutations in genes encoding either structural constituents of the cuticle itself (mostly collagen genes) or protein with regulatory functions. By using CRISPR\/Cas9 we confirmed 6 novel alleles of ptr-18, paqr-2, nab-1, ncam-1, vab-9 and efn-4. We further characterized the loss of function allele ptr-18(et70), which encodes a patch domain-containing (PTCHD) protein homologous to human PTCHD1. ptr-18(et70) has a significant effect on growth and development of the worms, while also increasing membrane permeability. Lipidomics analysis revealed no major alterations in membrane lipid composition, implicating cuticle defects as the primary cause of the observed permeability phenotype.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag070","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T19:01:37Z","timestamp":1776884497000},"source":"Crossref","is-referenced-by-count":0,"title":["Forward genetics identifies cuticle and regulatory genes underlying cellular boundary integrity in\n                    <i>C. elegans<\/i>"],"prefix":"10.1093","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9008-0202","authenticated-orcid":false,"given":"Uro\u0161","family":"Radovi\u0107","sequence":"first","affiliation":[{"name":"Department of Chemistry and Molecular Biology, University of Gothenburg , Gothenburg 405 30 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