{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:01:54Z","timestamp":1791378114802,"version":"4.3.2"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015094","name":"Ahmedabad University","doi-asserted-by":"publisher","award":["AU\/SUG\/DBLS\/2017-18\/03"],"award-info":[{"award-number":["AU\/SUG\/DBLS\/2017-18\/03"]}],"id":[{"id":"10.13039\/501100015094","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ramanujan Fellowship","award":["SB\/S2\/RJN-129\/2017"],"award-info":[{"award-number":["SB\/S2\/RJN-129\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Drosophila nepalensis is a cold-adapted drosophilid endemic to the Himalayan region. Its ability to survive in harsh, cold conditions makes it a valuable Drosophila model for investigating how adaptation to thermal extremes may influence species persistence under future climate change. Here, we report the first de novo genome assembly of D. nepalensis, based on a hybrid sequencing strategy that combines Illumina short reads and Oxford Nanopore long reads. Illumina sequencing generated 49.88 million 150 bp paired-end reads (\u223c14.96 Gbp), while Nanopore sequencing produced 1.35 million long reads totaling \u223c0.76 Gbp. The assembled genome spanned \u223c178 Mb with an N50 of 83.6 kb and 98% BUSCO completeness, comparable to other well-annotated Drosophila genomes. Annotation identified 10,560 protein-coding genes, including transcription factor\u2013rich and stress-related domains such as zinc fingers, WD40 repeats, and ankyrin motifs. Comparative orthology analysis across 6 Drosophila species identified 14,168 orthologous clusters, of which 9,173 were shared among all 6 species, indicating a conserved core genomic set across the sampled taxa. D. nepalensis showed 83 unique orthogroups and 50 singletons, suggesting some lineage-specific gene expansions associated with cold adaptation and endemicity, including families encoding caspase-family apoptotic regulators, chromatin remodeling proteins (HMGB\/protamine-like), and SNARE-domain vesicle trafficking factors. Gene family evolution analysis revealed the highest expansions in the cold-tolerant Himalayan drosophilid, D. nepalensis, including significant expansions in serine protease, chaperone, and neurotransmitter transporter families, alongside dramatic contractions of core histone gene families, suggesting lineage-specific chromatin remodeling and ecological specialization.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag190","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:59:11Z","timestamp":1788458351000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic signatures of cold adaptation in a Himalayan drosophilid"],"prefix":"10.1093","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7526-0663","authenticated-orcid":false,"given":"Avirup","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Division of Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University , Commerce Six Road, Navrangpura, Ahmedabad, Gujarat 380009 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9149-391X","authenticated-orcid":false,"given":"Subhash","family":"Rajpurohit","sequence":"additional","affiliation":[{"name":"Division of Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University , Commerce Six Road, Navrangpura, Ahmedabad, Gujarat 380009 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"2026100708173536700_jkag190-B1","doi-asserted-by":"publisher","first-page":"3148","DOI":"10.1093\/molbev\/msu246","article-title":"Drosophila suzukii: the genetic footprint of a recent, worldwide invasion","volume":"31","author":"Adrion","year":"2014","journal-title":"Mol Biol 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