{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T11:31:28Z","timestamp":1793791888760,"version":"4.6.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000015","name":"United States Department of Energy","doi-asserted-by":"publisher","award":["DE-SC0021286"],"award-info":[{"award-number":["DE-SC0021286"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We investigated the impact of ethyl methanesulfonate (EMS) on genetically effective cells (GECs) to characterize the intraindividual genetic variation of EMS mutagenesis in the novel intermediate crop pennycress (Thlaspi arvense L.). We identified that pennycress contains at least four GECs which, when treated with EMS, create unique mutations across different branches within the same individual plant. However, we also found that branches were not completely distinct with 3.83% to 34.2% of the observed mutations being shared between at least two branches. We then propagated the M2 plants for whole genome sequencing, providing extensive characterization of the EMS mutation profile and developing a gene index as a resource for future reverse genetic screenings. The gene index was then validated through identification of two traits. We identified five mutations in transparent testa (TT) family of genes which resulted in lighter seed coat coloration and two mutations in the indehiscent (IND) gene which resulted in increased shatter resistance of the pennycress seedpod. These two investigations demonstrate the viability of the gene index as a resource for further reverse genetic screenings.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag233","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T10:46:39Z","timestamp":1787395599000},"source":"Crossref","is-referenced-by-count":0,"title":["Characterization of genetically effective cells and EMS mutagenesis on the novel winter oil seed pennycress (\n                    <i>Thlaspi arvense<\/i>\n                    )"],"prefix":"10.1093","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5694-1561","authenticated-orcid":false,"given":"Anthony","family":"Brusa","sequence":"first","affiliation":[{"name":"Department of Agronomy and Plant Genetics, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratan","family":"Chopra","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Genetics, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva Serena","family":"Gjesvold","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Ott","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Genetics, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam","family":"Sullivan","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire Chapman","family":"Biel","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Jain","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Branch","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greta","family":"Rockstad","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishan M","family":"Rai","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Marks","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, University of Minnesota Twin Cities , St Paul, MN 55108 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,22]]},"reference":[{"key":"2026110406222151100_jkag233-B1","doi-asserted-by":"publisher","first-page":"D609","DOI":"10.1093\/nar\/gkae1010","article-title":"UniProt: the universal protein knowledgebase in 2025","volume":"53","author":"Bateman","year":"2025","journal-title":"Nucleic Acids 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