{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:42:43Z","timestamp":1792651363896,"version":"4.5.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the presence of glucose, Saccharomyces cerevisiae prefers glycolysis followed by fermentation to generate ATP. One adaptation to this fermentative lifestyle is the retrograde pathway (RTG), which induces the transcription of genes, including several Krebs cycle genes, to increase pools of alpha-ketoglutarate. We previously found that both petite and RTG mutants are highly sensitive to canavanine, a toxic analog of arginine, indicating that residual respiration and the RTG pathway are required to maintain arginine levels in the cell. Yet, from an amino acid biosynthesis standpoint, it is unclear how the RTG pathway balances between respiration and fermentation. Here, we show that use of a non-fermentable carbon source restored RTG mutants\u2019 ability to grow without arginine or lysine and improved their growth on canavanine and thialysine. Relief of glucose repression via deletion of MIG1 improved RTG mutant growth on agar under arginine or lysine limitation but was not sufficient to restore growth on a toxic analog. rtg2\u0394 exhibited more canavanine sensitivity than other RTG mutants, was not rescued by mig1\u0394, and was only partially rescued by mks1\u0394, suggesting that Rtg2 may have an additional function required for canavanine tolerance. Target of rapamycin (TOR) signaling as a pro-growth factor suppresses the RTG pathway, but it is unknown if both pathways complement each other. We found that TOR signaling contributes to canavanine tolerance in an RTG-independent manner. The broad implications of our results are found in the importance of fermentative lifestyle under amino acid starvation as part of industrial and malignant ecosystems.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag261","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:23:52Z","timestamp":1792650232000},"source":"Crossref","is-referenced-by-count":0,"title":["Crosstalk between RTG and TOR pathways mediates adaptation to amino acid starvation during fermentative growth in\n                    <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1093","author":[{"given":"Marina","family":"Druseikis","sequence":"first","affiliation":[{"name":"Department of Plant Pathology and Microbiology, The Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University , 229 Herzl St., Rehovot 7610001 ,","place":["Israel"]},{"name":"Shmunis School of Biomedical and Cancer Research, Tel Aviv University , 55 Levanon St., Tel Aviv 6997801 ,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8481-990X","authenticated-orcid":false,"given":"Shay","family":"Covo","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology, The Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University , 229 Herzl St., Rehovot 7610001 ,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"2026102202234840600_jkag261-B1","doi-asserted-by":"publisher","first-page":"131","DOI":"10.3109\/10408418509104427","article-title":"alpha-aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes","volume":"12","author":"Bhattacharjee","year":"1985","journal-title":"Crit Rev Microbiol."},{"key":"2026102202234840600_jkag261-B2","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/0022-2836(88)90574-8","article-title":"CYP1 (HAP1) regulator of oxygen-dependent gene expression in yeast. I. 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