{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:05:00Z","timestamp":1792785900973,"version":"4.5.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Education Alliance GENES"},{"name":"Gene-function Exploration by a Network of Emerging Scientists"},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"name":"School of Biological, Environmental, and Earth Sciences at the University of Southern Mississippi","id":[{"id":"https:\/\/ror.org\/0270vfa57","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bacteriophages encode numerous genes with no known function, many of which can affect essential cellular processes when expressed in the bacterial host. For mycobacteriophages, genome-wide overexpression in Mycobacterium smegmatis can be used to identify proteins that impair growth. To evaluate the cytotoxic potential of the Cluster P3 phage Xavia, we constructed a plasmid library containing 71 predicted Xavia genes under the anhydrotetracycline-inducible promoter pTet and screened this library in a plate-based cytotoxicity assay to measure impacts on M. smegmatis growth. Two genes prevented transformant recovery, consistent with toxicity under basal promoter leakiness, and inducible expression of 18 additional genes impaired growth. These inhibitory proteins include structural components; factors involved in DNA metabolism, lysogeny, and lysis; and several proteins with no known function. These results extend functional screening into a lineage of actinobacteriophages that has not previously been characterized, and identify new proteins that warrant further mechanistic analysis.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag262","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:45:49Z","timestamp":1789577149000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification of novel inhibitors of\n                    <i>Mycobacterium smegmatis<\/i>\n                    growth through genome-wide overexpression of Cluster P3 mycobacteriophage Xavia genes"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8421-5951","authenticated-orcid":false,"given":"Anushka","family":"Tennakoon","sequence":"first","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences, University of Southern Mississippi , Hattiesburg, MS 39406 ,","place":["United 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