{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T12:24:54Z","timestamp":1793795094596,"version":"4.6.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Spanish Ministerio de Ciencia e Innovacio\u0301n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigacio\u0301n","doi-asserted-by":"publisher","award":["PID2021-124716OB-I00"],"award-info":[{"award-number":["PID2021-124716OB-I00"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100030956","name":"Fundacio\u0301 Sant Joan de De\u0301u per la Recerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The conversion of transcriptionally silent GGAA microsatellites (GGAA\u00b5Sats) into functional enhancers by FET::ETS oncogenic fusions is a hallmark of Ewing sarcoma. However, emerging evidence implicates nonfused, full-length oncogenic E26 transformation-specific (ETS) transcription factors in activating these repeats in other malignancies. Evaluating the in vivo transcriptional requirements of various human ETS factors in Drosophila, we found that human ETS variant transcription factor 4 (ETV4) associates with and robustly activates GGAA\u00b5Sats in a tissue-specific manner. This activation is strongly inhibited by the human ETS repressor ETS variant transcription factor 6 (ETV6). Taking advantage of low genetic redundancy in Drosophila, we identified Cabeza (Caz), the single fly FUS\/EWSR1\/TAF15 family (FET) ortholog, as a necessary cofactor for ETV4-mediated transcription at GGAA\u00b5Sats. Conversely, EWS::FLI1-mediated transcriptional activation of GGAA\u00b5Sats is entirely independent of endogenous Caz, highlighting the distinct mechanics of covalent tethering vs noncovalent physical complexes. Collectively, our findings provide in vivo evidence that nonfused ETS factors cooperate with endogenous FET proteins to drive transcription from silent GGAA repeats, mechanistically validating this regulatory transformation known to operate as an oncogenic mechanism beyond Ewing sarcoma.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag265","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:33:02Z","timestamp":1790101982000},"source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <i>Drosophila<\/i>\n                    FET ortholog Cabeza is an essential cofactor for ETV4-mediated activation of GGAA microsatellite neoenhancers"],"prefix":"10.1093","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3426-4234","authenticated-orcid":false,"given":"Cristina","family":"Molnar","sequence":"first","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology , Barcelona 08028 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Reina","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology , Barcelona 08028 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaume","family":"Mora","sequence":"additional","affiliation":[{"name":"Developmental Tumor Biology Laboratory, Institut de Recerca Sant Joan de D\u00e9u , Esplugues de Llobregat, Barcelona 08950 ,","place":["Spain"]},{"name":"Pediatric Cancer Center Barcelona (PCCB), Hospital Sant Joan de D\u00e9u Barcelona , Esplugues de Llobregat, Barcelona 08950 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5259-3608","authenticated-orcid":false,"given":"Cayetano","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Institute for 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