{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:47:50Z","timestamp":1791974870351,"version":"4.4.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32400501"],"award-info":[{"award-number":["32400501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>MicroRNAs are essential for worm development, yet their roles remain unclear in most tissues. Here, we established a tissue-specific Argonaute knockout system in Caenorhabditis elegans using conditional deletion of alg-1 in an alg-2 mutant background. Tissue-restricted loss of Argonaute revealed distinct developmental and tissue-specific phenotypes. Argonaute depletion in the intestine, pharynx, and hypodermis caused developmental arrest and tissue-specific defects, whereas neuronal and muscle-specific Argonaute depletion impaired locomotion and affected neuronal or muscle-associated phenotypes. Moreover, combined loss of selected tissue-expressed microRNAs only partially recapitulated the corresponding Argonaute-depletion phenotypes. Muscle-specific re-expression of selected microRNAs rescued the locomotion defect of the compound microRNA mutant. This study establishes a platform for dissecting tissue-specific microRNA functions during animal development.<\/jats:p>","DOI":"10.1093\/g3journal\/jkag279","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:13:56Z","timestamp":1790756036000},"source":"Crossref","is-referenced-by-count":0,"title":["Conditional Argonaute knockout reveals tissue-specific requirements of microRNA activity in\n                    <i>C. elegans<\/i>"],"prefix":"10.1093","author":[{"given":"Yimeng","family":"Gao","sequence":"first","affiliation":[{"name":"College of Life Sciences, Capital Normal University , Beijing 100048 ,","place":["China"]},{"name":"School of Life Sciences, Tsinghua University , Beijing 100084 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchuan","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Capital Normal University , Beijing 100048 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokun","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Capital Normal University , Beijing 100048 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiru","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Capital Normal University , Beijing 100048 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0686-0883","authenticated-orcid":false,"given":"Yongbin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Capital Normal University , Beijing 100048 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