{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:15:26Z","timestamp":1791134126928,"version":"4.1.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T00:00:00Z","timestamp":1708560000000},"content-version":"vor","delay-in-days":62,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Biliary tract infection is a common complication of choledocholithiasis. This study aimed to analyse the distribution of pathogenic bacteria in bile cultures from patients with choledocholithiasis combined with biliary tract infection to guide clinical application of antimicrobials and reduce the emergence of drug resistance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 880 patients were enrolled in this retrospective study from 30 March 2017 to 31 August 2022 at the Affiliated Hospital of Qingdao University in China. Bile specimens were extracted for microbiological culture under aseptic conditions using endoscopic retrograde cholangiopancreatography. Bacterial culture, strain identification, and antimicrobial susceptibility testing were conducted according to the standard protocol. Baseline data were retrieved from patient files.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Overall, 90.34% (795\/880) of bile samples showed positive microbiological results and 37.50% (330\/880) demonstrated polymicrobial infections. Among the 795 bile specimens with positive culture results, 1,216 pathogenic bacteria were detected, with gram-negative bacilli accounting for 56.33%, gram-positive cocci for 41.86%, and fungi for 1.81%. The predominant gram-negative bacilli in the bile cultures were Escherichia coli (30.43%) and Klebsiella pneumoniae (13.98%), whereas the main gram-positive cocci were Enterococcus faecium (14.04%) and E. casseliflavus (4.28%). The annual trend analysis revealed a gradual decrease in the proportion of gram-negative bacilli and a gradual increase in the proportion of gram-positive cocci, with a concomitant decrease in the dominance of E. coli. Both E. faecium and E. coli showed high resistance to conventional antibiotics but high sensitivity to piperacillin\/tazobactam, carbapenems, amikacin, and vancomycin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A significant change has occurred in the bile bacterial spectrum in patients with choledocholithiasis and biliary tract infection. The incidence of gram-positive cocci infections has increased annually, while that of gram-negative bacilli and E. coli infections has decreased. Antibiotic administration should be tailored based on the local bacterial profile.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/gastro\/goae010","type":"journal-article","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T09:24:24Z","timestamp":1707211464000},"source":"Crossref","is-referenced-by-count":13,"title":["Variability of bile bacterial profiles and drug resistance in patients with choledocholithiasis combined with biliary tract infection: a retrospective study"],"prefix":"10.1093","volume":"12","author":[{"given":"Hang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, P. R. China"},{"name":"Department of Medicine, Qingdao University , Qingdao, Shandong, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, P. R. China"},{"name":"Department of Medicine, Qingdao University , Qingdao, Shandong, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichao","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Radiology, Baylor University , Waco, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuijin","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, P. R. China"},{"name":"Department of Medicine, Qingdao University , Qingdao, Shandong, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, P. R. 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