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However, the mechanisms of mucosal repair that are regulated by ISCs in ulcerative colitis (UC) remain unclear.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Method<\/jats:title>\n                  <jats:p>Colon tissues from patients with UC were collected to test \u03b2-catenin and Notch1 expression by using Western blot and quantitative real-time polymerase chain reaction (PCR). \u03b2-cateninfl\/fl mice, \u03b2-cateninTg mice, and Dll1tm1 Gos mice were used to cross with Lgr5-EGFP-IRES-creERT2 mice to generate mice of different genotypes, altering the activation of Wnt\/\u03b2-catenin and Dll1-mediated Notch signaling in ISCs in vivo. Dextran sulfate sodium (DSS) was used to induce a colitis mice model. Intestinal organoids were isolated and cultured to observe the proliferation and differentiation levels of ISCs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Result<\/jats:title>\n                  <jats:p>\u03b2-catenin and Notch1 expression were significantly increased in the inflamed colon tissues from patients with UC. Wnt\/\u03b2-catenin activation and Dll1-mediated Notch pathway inhibition in Lgr5-positive stem cells promoted the expressions of E-cadherin, CK20, and CHGA in colonic organoids and epithelium, implying the promotion of colonic epithelial integrity. Activation of Wnt\/\u03b2-catenin and suppression of Dll1-mediated Notch pathway in Lgr5-positive ISCs alleviated the DSS-induced intestinal mucosal inflammation in mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Lgr5-positive ISCs are characterized by self-renewal and high dividend potential, which play an important role in the injury and repair of intestinal mucosa. More importantly, the Wnt\/\u03b2-catenin signaling pathway cooperates with the Notch signaling pathway to maintain the function of the Lgr5-positive ISCs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/gastro\/goae090","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T20:59:26Z","timestamp":1729717166000},"source":"Crossref","is-referenced-by-count":12,"title":["Cooperation of Wnt\/\u03b2-catenin and Dll1-mediated Notch pathway in Lgr5-positive intestinal stem cells regulates the mucosal injury and repair in DSS-induced colitis mice model"],"prefix":"10.1093","volume":"12","author":[{"given":"Weijun","family":"Ou","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiaotong University, School of Medicine , Shanghai,","place":["P. R. China"]},{"name":"Shanghai Colorectal Cancer Research Center , Shanghai,","place":["P. R. 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