{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:14:18Z","timestamp":1784074458948,"version":"3.55.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Frontiers in Science"},{"name":"Royal Society Newton"},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The water skinks Eulamprus tympanum and Eulamprus heatwolei show thermally induced sex determination where elevated temperatures give rise to male offspring. Paradoxically, Eulamprus species reproduce in temperatures of 12\u201315\u2009\u00b0C making them outliers when compared with reptiles that use temperature as a cue for sex determination. Moreover, these two species are among the very few viviparous reptiles reported to have thermally induced sex determination. Thus, we tested whether these skinks possess undetected sex chromosomes with thermal override. We produced transcriptome and genome data for E.\u00a0heatwolei. We found that E.\u00a0heatwolei presents XY chromosomes that include 14 gametologs with regulatory functions. The Y chromosomal region is 79\u2013116\u00a0Myr old and shared between water and spotted skinks. Our work provides clear evidence that climate could be useful to predict the type of sex determination systems in reptiles and it also indicates that viviparity is strictly associated with sex chromosomes.<\/jats:p>","DOI":"10.1093\/gbe\/evaa104","type":"journal-article","created":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T12:03:45Z","timestamp":1589544225000},"page":"924-930","source":"Crossref","is-referenced-by-count":48,"title":["Viviparous Reptile Regarded to Have Temperature-Dependent Sex Determination Has Old XY Chromosomes"],"prefix":"10.1093","volume":"12","author":[{"given":"Paola","family":"Cornejo-P\u00e1ramo","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, UNAM, Cuernavaca, M\u00e9xico"},{"name":"Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duminda S B","family":"Dissanayake","sequence":"first","affiliation":[{"name":"Institute for Applied Ecology, University of Canberra, Australia"},{"name":"CSIRO, Australian National Wildlife Collection, Canberra, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Lira-Noriega","sequence":"first","affiliation":[{"name":"CONACYT Research Fellow, Red de Estudios Moleculares Avanzados, Instituto de Ecolog\u00eda, A.C. Carretera antigua a Coatepec 351, Xalapa, Veracruz, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica L","family":"Mart\u00ednez-Pacheco","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, UNAM, Cuernavaca, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando","family":"Acosta","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, UNAM, Cuernavaca, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciro","family":"Ram\u00edrez-Su\u00e1stegui","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, UNAM, Cuernavaca, M\u00e9xico"},{"name":"Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fausto R","family":"M\u00e9ndez-de-la-Cruz","sequence":"first","affiliation":[{"name":"Biology Institute, UNAM, Mexico City, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam\u00e1s","family":"Sz\u00e9kely","sequence":"first","affiliation":[{"name":"Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, United Kingdom"},{"name":"Department of Evolutionary Zoology and Human Biology, University of Debrecen, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araxi O","family":"Urrutia","sequence":"first","affiliation":[{"name":"Department of Biology and Biochemistry, Milner Centre for Evolution, University of Bath, United Kingdom"},{"name":"Institute of Ecology, UNAM, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2428-0361","authenticated-orcid":false,"given":"Arthur","family":"Georges","sequence":"first","affiliation":[{"name":"Institute for Applied Ecology, University of Canberra, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Cortez","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, UNAM, Cuernavaca, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,5,20]]},"reference":[{"issue":"9","key":"2020062411334473800_evaa104-B1","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1093\/gbe\/evz196","article-title":"Corytophanids replaced the pleurodont XY system with a new pair of XY chromosomes","volume":"11","author":"Acosta","year":"2019","journal-title":"Genome Biol Evol"},{"issue":"6209","key":"2020062411334473800_evaa104-B2","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1126\/science.1257225","article-title":"Plant genetics. A Y-chromosome-encoded small RNA acts as a sex determinant in persimmons","volume":"346","author":"Akagi","year":"2014","journal-title":"Science"},{"issue":"4","key":"2020062411334473800_evaa104-B3","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1111\/j.1420-9101.2006.01086.x","article-title":"Do operational sex ratios influence sex allocation in viviparous lizards with temperature-dependent sex determination?","volume":"19","author":"Allsop","year":"2006","journal-title":"J Evol Biol"},{"issue":"3","key":"2020062411334473800_evaa104-B4","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"issue":"7","key":"2020062411334473800_evaa104-B5","doi-asserted-by":"crossref","first-page":"e1001899","DOI":"10.1371\/journal.pbio.1001899","article-title":"Sex determination: why so many ways of doing it?","volume":"12","author":"Bachtrog","year":"2014","journal-title":"PLoS Biol"},{"issue":"15","key":"2020062411334473800_evaa104-B6","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"issue":"5605","key":"2020062411334473800_evaa104-B7","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/266828a0","article-title":"When is sex environmentally determined?","volume":"266","author":"Charnov","year":"1977","journal-title":"Nature"},{"key":"2020062411334473800_evaa104-B8","doi-asserted-by":"crossref","first-page":"e6281","DOI":"10.7717\/peerj.6281","article-title":"kuenm: an R package for detailed development of ecological niche models using Maxent","volume":"7","author":"Cobos","year":"2019","journal-title":"PeerJ"},{"key":"2020062411334473800_evaa104-B9","author":"Cogger","year":"2000"},{"issue":"7497","key":"2020062411334473800_evaa104-B10","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/nature13151","article-title":"Origins and functional evolution of Y chromosomes across mammals","volume":"508","author":"Cortez","year":"2014","journal-title":"Nature"},{"issue":"7","key":"2020062411334473800_evaa104-B11","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat Biotechnol"},{"issue":"4","key":"2020062411334473800_evaa104-B12","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1093\/gbe\/evy042","article-title":"Conservation of sex-linked markers among conspecific populations of a viviparous skink, Niveoscincus ocellatus, exhibiting genetic and temperature-dependent sex determination","volume":"10","author":"Hill","year":"2018","journal-title":"Genome Biol Evol"},{"issue":"7558","key":"2020062411334473800_evaa104-B13","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nature14574","article-title":"Sex reversal triggers the rapid transition from genetic to temperature-dependent sex","volume":"523","author":"Holleley","year":"2015","journal-title":"Nature"},{"issue":"4","key":"2020062411334473800_evaa104-B14","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"issue":"9","key":"2020062411334473800_evaa104-B15","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1111\/evo.12130","article-title":"Evolution of viviparity: a phylogenetic test of the cold-climate hypothesis in phrynosomatid lizards","volume":"67","author":"Lambert","year":"2013","journal-title":"Evolution"},{"issue":"4","key":"2020062411334473800_evaa104-B16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat Methods"},{"issue":"30","key":"2020062411334473800_evaa104-B17","doi-asserted-by":"crossref","first-page":"10557","DOI":"10.1073\/pnas.0409137102","article-title":"An algorithm for progressive multiple alignment of sequences with insertions","volume":"102","author":"Loytynoja","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"2020062411334473800_evaa104-B18","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1101\/gr.223727.117","article-title":"Convergent origination of a Drosophila-like dosage compensation mechanism in a reptile lineage","volume":"27","author":"Marin","year":"2017","journal-title":"Genome Res"},{"issue":"4","key":"2020062411334473800_evaa104-B19","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1186\/gb-2010-11-4-205","article-title":"Are homologies in vertebrate sex determination due to shared ancestry or to limited options?","volume":"11","author":"Marshall Graves","year":"2010","journal-title":"Genome Biol"},{"issue":"1","key":"2020062411334473800_evaa104-B20","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s10577-011-9266-8","article-title":"Are some chromosomes particularly good at sex? Insights from amniotes","volume":"20","author":"O\u2019Meally","year":"2012","journal-title":"Chromosome Res"},{"issue":"3","key":"2020062411334473800_evaa104-B21","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1093\/bioinformatics\/bty633","article-title":"ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R","volume":"35","author":"Paradis","year":"2019","journal-title":"Bioinformatics"},{"issue":"7322","key":"2020062411334473800_evaa104-B22","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/nature09512","article-title":"Climate-driven population divergence in sex-determining systems","volume":"468","author":"Pen","year":"2010","journal-title":"Nature"},{"issue":"3\u20134","key":"2020062411334473800_evaa104-B23","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.ecolmodel.2005.03.026","article-title":"Maximum entropy modeling of species geographic distributions","volume":"190","author":"Phillips","year":"2006","journal-title":"Ecol Model"},{"issue":"1","key":"2020062411334473800_evaa104-B24","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/ele.12168","article-title":"Early origin of viviparity and multiple reversions to oviparity in squamate reptiles","volume":"17","author":"Pyron","year":"2014","journal-title":"Ecol Lett"},{"issue":"6","key":"2020062411334473800_evaa104-B25","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"issue":"5823","key":"2020062411334473800_evaa104-B26","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1126\/science.1135925","article-title":"Temperature sex reversal implies sex gene dosage in a reptile","volume":"316","author":"Quinn","year":"2007","journal-title":"Science"},{"issue":"2","key":"2020062411334473800_evaa104-B27","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1098\/rsbl.2007.0583","article-title":"Genetic evidence for co-occurrence of chromosomal and thermal sex-determining systems in a lizard","volume":"4","author":"Radder","year":"2008","journal-title":"Biol Lett"},{"issue":"6848","key":"2020062411334473800_evaa104-B28","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/35089135","article-title":"Sex determination. Viviparous lizard selects sex of embryos","volume":"412","author":"Robert","year":"2001","journal-title":"Nature"},{"issue":"4","key":"2020062411334473800_evaa104-B29","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1007\/BF00317720","article-title":"Thermal biology of reproduction in viviparous skinks, Eulamprus tympanum: why do gravid females bask more?","volume":"88","author":"Schwarzkopf","year":"1991","journal-title":"Oecologia"},{"issue":"5","key":"2020062411334473800_evaa104-B30","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0169-5347(98)01575-4","article-title":"Why is sex determined by nest temperature in many reptiles?","volume":"14","author":"Shine","year":"1999","journal-title":"Trends Ecol Evol"},{"issue":"4","key":"2020062411334473800_evaa104-B31","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1046\/j.1461-0248.2002.00351.x","article-title":"Co-occurrence of multiple, supposedly incompatible modes of sex determination in a lizard population","volume":"5","author":"Shine","year":"2002","journal-title":"Ecol Lett"},{"issue":"2","key":"2020062411334473800_evaa104-B32","doi-asserted-by":"crossref","first-page":"136","DOI":"10.2174\/1874467208666150126154222","article-title":"Phosphoprotein phosphatase 1 complexes in spermatogenesis","volume":"7","author":"Silva","year":"2015","journal-title":"Curr Mol Pharmacol"},{"issue":"1","key":"2020062411334473800_evaa104-B33","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-ecolsys-102710-145051","article-title":"Phylogenetic insights on evolutionary novelties in lizards and snakes: sex, birth, bodies, niches, and venom","volume":"42","author":"Sites","year":"2011","journal-title":"Annu Rev Ecol Evol Syst"},{"key":"2020062411334473800_evaa104-B34","doi-asserted-by":"crossref","first-page":"140015","DOI":"10.1038\/sdata.2014.15","article-title":"Tree of sex: a database of sexual systems","volume":"1","author":"Tree of Sex","year":"2014","journal-title":"Sci Data"},{"issue":"8","key":"2020062411334473800_evaa104-B35","doi-asserted-by":"crossref","first-page":"e1001643","DOI":"10.1371\/journal.pbio.1001643","article-title":"Comparative sex chromosome genomics in snakes: differentiation, evolutionary strata, and lack of global dosage compensation","volume":"11","author":"Vicoso","year":"2013","journal-title":"PLoS Biol"},{"issue":"5","key":"2020062411334473800_evaa104-B36","first-page":"555","article-title":"PAML: a program package for phylogenetic analysis by maximum likelihood","volume":"13","author":"Yang","year":"1997","journal-title":"Comput Appl Biosci"}],"container-title":["Genome Biology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/gbe\/advance-article-pdf\/doi\/10.1093\/gbe\/evaa104\/33242282\/evaa104.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/gbe\/article-pdf\/12\/6\/924\/33424272\/evaa104.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/gbe\/article-pdf\/12\/6\/924\/33424272\/evaa104.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T15:36:55Z","timestamp":1593013015000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/gbe\/article\/12\/6\/924\/5841216"}},"subtitle":[],"editor":[{"given":"Judith","family":"Mank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,5,20]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/gbe\/evaa104","relation":{},"ISSN":["1759-6653"],"issn-type":[{"value":"1759-6653","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,6]]},"published":{"date-parts":[[2020,5,20]]}}}