{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:30:08Z","timestamp":1791214208869,"version":"4.1.0"},"reference-count":86,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["1039\/12"],"award-info":[{"award-number":["1039\/12"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["484\/17"],"award-info":[{"award-number":["484\/17"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003483","name":"Hebrew University of Jerusalem","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003483","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) are a superfamily of small phloem-feeding insects. They rely on their primary endosymbionts \"Candidatus Portiera aleyrodidarum\" to produce essential amino acids not present in their diet. Portiera has been codiverging with whiteflies since their origin and therefore reflects its host\u2019s evolutionary history. Like in most primary endosymbionts, the genome of Portiera stays stable across the Aleyrodidae superfamily after millions of years of codivergence. However, Portiera of the whitefly Bemisia tabaci has lost the ancestral genome order, reflecting a rare event in the endosymbiont evolution: the appearance of genome instability. To gain a better understanding of Portiera genome evolution, identify the time point in which genome instability appeared and contribute to the reconstruction of whitefly phylogeny, we developed a new phylogenetic framework. It targeted five Portiera genes and determined the presence of the DNA polymerase proofreading subunit (dnaQ) gene, previously associated with genome instability, and two alternative gene rearrangements. Our results indicated that Portiera gene sequences provide a robust tool for studying intergenera phylogenetic relationships in whiteflies. Using these new framework, we found that whitefly species from the Singhiella, Aleurolobus, and Bemisia genera form a monophyletic tribe, the Aleurolobini, and that their Portiera exhibit genome instability. This instability likely arose once in the common ancestor of the Aleurolobini tribe (at least 70 Ma), drawing a link between the appearance of genome instability in Portiera and the switch from multibacteriocyte to a single-bacteriocyte mode of inheritance in this tribe.<\/jats:p>","DOI":"10.1093\/gbe\/evaa216","type":"journal-article","created":{"date-parts":[[2020,10,10]],"date-time":"2020-10-10T11:37:30Z","timestamp":1602329850000},"page":"2107-2124","source":"Crossref","is-referenced-by-count":19,"title":["<i>Portiera<\/i> Gets Wild: Genome Instability Provides Insights into the Evolution of Both Whiteflies and Their Endosymbionts"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3401-2662","authenticated-orcid":false,"given":"Diego","family":"Santos-Garcia","sequence":"first","affiliation":[{"name":"Department of Entomology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natividad","family":"Mestre-Rincon","sequence":"additional","affiliation":[{"name":"Department of Entomology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2931-6116","authenticated-orcid":false,"given":"David","family":"Ouvrard","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Natural History Museum, London, United Kingdom"},{"name":"Entomology and Invasive Plants Unit, Plant Health Laboratory, ANSES, Montferrier-sur-Lez, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Einat","family":"Zchori-Fein","sequence":"additional","affiliation":[{"name":"Department of Entomology, Newe-Ya\u2019ar Research Center, ARO, Ramat-Yishai, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2157-1999","authenticated-orcid":false,"given":"Shai","family":"Morin","sequence":"additional","affiliation":[{"name":"Department of Entomology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,13]]},"reference":[{"issue":"11","key":"2021012211100302300_evaa216-B1","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1093\/molbev\/msx197","article-title":"Large variation in the ratio of mitochondrial to nuclear mutation rate across animals: implications for genetic diversity and the use of mitochondrial DNA as a molecular marker","volume":"34","author":"Allio","year":"2017","journal-title":"Mol Biol Evol"},{"issue":"5","key":"2021012211100302300_evaa216-B2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J Comput 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