{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:00:58Z","timestamp":1781100058391,"version":"3.54.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T00:00:00Z","timestamp":1613606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Legionella spp. are ubiquitous bacteria principally found in water networks and \u223c20 species are implicated in Legionnaire\u2019s disease. Among them, Legionella pneumophila is an intracellular pathogen of environmental protozoa, responsible for \u223c90% of cases in the world. Legionella pneumophila regulates in part its virulence by a quorum sensing system named \u201cLegionella quorum sensing,\u201d composed of a signal synthase LqsA, two histidine kinase membrane receptors LqsS and LqsT and a cytoplasmic receptor LqsR. To date, this communication system was only found in L.\u00a0pneumophila. Here, we investigated 58 Legionella genomes to determine the presence of a lqs cluster or homologous receptors using TBlastN. This analysis revealed three categories of species: 19 harbored a complete lqs cluster, 20 did not possess lqsA but maintained the receptor lqsR and\/or lqsS, and 19 did not have any of the lqs genes. No correlation was observed between pathogenicity and the presence of a quorum sensing system. We determined by RT-qPCR that the lqsA gene was expressed at least in four strains among different species available in our laboratory. Furthermore, we showed that the lqs genomic region was conserved even in species possessing only the receptors of the quorum sensing system, indicating an ancestral acquisition and various loss dynamics during evolution. This system could therefore function in interspecific communication as well.<\/jats:p>","DOI":"10.1093\/gbe\/evab032","type":"journal-article","created":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T12:46:05Z","timestamp":1613393165000},"source":"Crossref","is-referenced-by-count":8,"title":["<i>Legionella<\/i> spp. All Ears? The Broad Occurrence of Quorum Sensing Elements outside <i>Legionella pneumophila<\/i>"],"prefix":"10.1093","volume":"13","author":[{"given":"Benjamin","family":"Herran","sequence":"first","affiliation":[{"name":"Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Gr\u00e8ve","sequence":"additional","affiliation":[{"name":"Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Berjeaud","sequence":"additional","affiliation":[{"name":"Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Bertaux","sequence":"additional","affiliation":[{"name":"Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Cr\u00e9pin","sequence":"additional","affiliation":[{"name":"Laboratoire Ecologie & Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,2,18]]},"reference":[{"issue":"5","key":"2021041418131200200_evab032-B1","doi-asserted-by":"crossref","first-page":"750","DOI":"10.3201\/eid2305.161165","article-title":"Population genomics of Legionella longbeachae and hidden complexities of infection source attribution","volume":"23","author":"Bacigalupe","year":"2017","journal-title":"Emerg Infect Dis"},{"key":"2021041418131200200_evab032-B2","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.mib.2018.02.002","article-title":"Stimulus sensing and signal processing in bacterial chemotaxis","volume":"45","author":"Bi","year":"2018","journal-title":"Curr Opin Microbiol"},{"issue":"2","key":"2021041418131200200_evab032-B3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF02602903","article-title":"Classification of two additional species of Legionella associated with human pneumonia","volume":"4","author":"Brenner","year":"1980","journal-title":"Curr Microbiol"},{"issue":"2","key":"2021041418131200200_evab032-B4","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/ng.3481","article-title":"Genomic analysis of 38 Legionella species identifies large and diverse effector repertoires","volume":"48","author":"Burstein","year":"2016","journal-title":"Nat Genet"},{"issue":"4","key":"2021041418131200200_evab032-B5","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1093\/oxfordjournals.molbev.a026334","article-title":"Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis","volume":"17","author":"Castresana","year":"2000","journal-title":"Mol Biol Evol"},{"key":"2021041418131200200_evab032-B6","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1038\/ng1447","article-title":"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity","volume":"36","author":"Cazalet","year":"2004","journal-title":"Nat Genet"},{"issue":"2","key":"2021041418131200200_evab032-B7","doi-asserted-by":"crossref","first-page":"e1000851","DOI":"10.1371\/journal.pgen.1000851","article-title":"Analysis of the Legionella longbeachae genome and transcriptome uncovers unique strategies to cause Legionnaires\u2019 disease","volume":"6","author":"Cazalet","year":"2010","journal-title":"PLoS Genet"},{"key":"2021041418131200200_evab032-B8","first-page":"4381","article-title":"Infection of macrophage-like cells by Legionella species that have not been associated with 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Res"},{"issue":"4","key":"2021041418131200200_evab032-B12","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1099\/00207713-30-4-609","article-title":"Tatlockia and Fluoribacter: two new genera of organisms resembling Legionella pneumophila","volume":"30","author":"Garrity","year":"1980","journal-title":"Int J Syst Bacteriol"},{"issue":"2","key":"2021041418131200200_evab032-B13","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/molbev\/msp259","article-title":"SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building","volume":"27","author":"Gascuel","year":"2010","journal-title":"Mol Biol Evol"},{"issue":"5","key":"2021041418131200200_evab032-B14","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/s41435-019-0074-z","article-title":"Intracellular parasitism, the driving force of evolution of Legionella pneumophila and the genus 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the formation of virulent Legionella persisters within infected cells","volume":"10","author":"Personnic","year":"2019","journal-title":"Nat Commun"},{"key":"2021041418131200200_evab032-B34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.mib.2017.11.010","article-title":"Legionella quorum sensing and its role in pathogen\u2013host interactions","volume":"41","author":"Personnic","year":"2018","journal-title":"Curr Opin Microbiol"},{"issue":"7","key":"2021041418131200200_evab032-B35","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1099\/mic.0.2008\/017871-0","article-title":"Diffusible signals and interspecies communication in bacteria","volume":"154","author":"Ryan","year":"2008","journal-title":"Microbiology"},{"issue":"3","key":"2021041418131200200_evab032-B36","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1093\/gbe\/evz046","article-title":"Distribution and genetic diversity of genes involved in quorum sensing and prodigiosin biosynthesis in the complete genome sequences of Serratia marcescens","volume":"11","author":"Sakuraoka","year":"2019","journal-title":"Genome Biol Evol"},{"issue":"4","key":"2021041418131200200_evab032-B37","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1111\/mmi.13265","article-title":"The \u03b1-hydroxyketone LAI-1 regulates motility, Lqs-dependent phosphorylation signalling and gene expression of Legionella pneumophila","volume":"99","author":"Schell","year":"2016","journal-title":"Mol Microbiol"},{"key":"2021041418131200200_evab032-B38","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/978-1-4939-9173-0_14","article-title":"BUSCO: assessing genome assembly and annotation completeness","volume":"1962","author":"Seppey","year":"2019","journal-title":"Methods Mol Biol"},{"issue":"26","key":"2021041418131200200_evab032-B39","doi-asserted-by":"crossref","first-page":"18113","DOI":"10.1074\/jbc.M801929200","article-title":"The Legionella autoinducer synthase LqsA produces an \u03b1-hydroxyketone signaling molecule","volume":"283","author":"Spirig","year":"2008","journal-title":"J Biol Chem"},{"key":"2021041418131200200_evab032-B40","first-page":"1312","author":"Stamatakis","year":"2014"},{"issue":"1","key":"2021041418131200200_evab032-B41","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1128\/AEM.56.1.49-53.1990","article-title":"Isolation of Legionella longbeachae serogroup 1 from potting mixes","volume":"56","author":"Steele","year":"1990","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"2021041418131200200_evab032-B42","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1128\/JCM.26.3.418-420.1988","article-title":"Legionella cincinnatiensis sp. nov. isolated from a patient with pneumonia","volume":"26","author":"Thacker","year":"1988","journal-title":"J Clin 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