{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:09:30Z","timestamp":1791497370630,"version":"4.3.4"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T00:00:00Z","timestamp":1626998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We here describe sequencing and assembly of both the autosomes and the sex chromosome in Micropoecilia picta, the closest related species to the guppy, Poecilia reticulata. Poecilia (Micropoecilia) picta is a close outgroup for studying the guppy, an important organism for studies in evolutionary ecology and in sex chromosome evolution. The guppy XY pair (LG12) has long been studied as a test case for the importance of sexually antagonistic variants in selection for suppressed recombination between Y and X chromosomes. The guppy Y chromosome is not degenerated, but appears to carry functional copies of all genes that are present on its X counterpart. The X chromosomes of M. picta (and its relative Micropoecilia parae) are homologous to the guppy XY pair, but their Y chromosomes are highly degenerated, and no genes can be identified in the fully Y-linked region. A complete genome sequence of a M. picta male may therefore contribute to understanding how the guppy Y evolved. These fish species\u2019 genomes are estimated to be about 750\u2009Mb, with high densities of repetitive sequences, suggesting that long-read sequencing is needed. We evaluated several assembly approaches, and used our results to investigate the extent of Y chromosome degeneration in this species.<\/jats:p>","DOI":"10.1093\/gbe\/evab171","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T19:11:23Z","timestamp":1626894683000},"source":"Crossref","is-referenced-by-count":9,"title":["PromethION Sequencing and Assembly of the Genome of <i>Micropoecilia picta<\/i>, a Fish with a Highly Degenerated Y Chromosome"],"prefix":"10.1093","volume":"13","author":[{"given":"Deborah","family":"Charlesworth","sequence":"first","affiliation":[{"name":"Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chay","family":"Graham","sequence":"additional","affiliation":[{"name":"Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, United Kingdom"},{"name":"Department of 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