{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:22:14Z","timestamp":1789129334489,"version":"build-2803163510"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"RQRAD"},{"DOI":"10.13039\/100008777","name":"MAPAQ","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008777","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003151","name":"FRQNT","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003151","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011202","name":"Programme de recherche en partenariat","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011202","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agriculture durable"},{"name":"Volet II"},{"name":"2e concours","award":["#337847"],"award-info":[{"award-number":["#337847"]}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"NSERC","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Alliance-SARI Program","award":["ALLRP 588519-23"],"award-info":[{"award-number":["ALLRP 588519-23"]}]},{"DOI":"10.13039\/501100003383","name":"CRC","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The potato leafhopper, Empoasca fabae (Harris, 1841), is a highly polyphagous, migratory insect pest of eastern North America that feeds on more than 200 herbaceous and woody plant species, causing substantial losses to forage and field crops. Despite its agricultural and ecological importance, no genome has been available for this species. Here, we present the first chromosome-level genome assembly of E. fabae, generated from Oxford Nanopore long reads, Illumina short reads, and Omni-C proximity-ligation data. The final assembly spans 908\u2005Mb across 132 scaffolds, with 99.8% of the assembly captured in ten chromosome-length scaffolds (nine autosomes and an X chromosome) with a scaffold N50 of 96.2\u2005Mb. The assembly is highly complete, recovering 92.9% of conserved hemipteran single-copy orthologs from protein annotations, and is composed of 47.6% repetitive sequence, dominated by long terminal repeat retrotransposons and unclassified elements. Read-depth comparison between male and female individuals supports assignment of a single sex-linked chromosome, consistent with an XO sex determination system. BRAKER3 gene annotation predicted 31,406 protein-coding genes after retaining the longest isoform per locus. Comparative genome analysis of the two closest related Typhlocybinae species with genomes available, Matsumurasca onukii and Hebata decipiens, revealed extensive chromosome-scale collinearity while defining a shared core gene repertoire. This reference genome provides a foundation for comparative and population genomic studies and for investigating genetic traits in this economically important crop pest species.<\/jats:p>","DOI":"10.1093\/gbe\/evag207","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:59:57Z","timestamp":1787050797000},"source":"Crossref","is-referenced-by-count":0,"title":["A Chromosome-Level Genome Assembly of the Potato Leafhopper\n                    <i>Empoasca fabae<\/i>\n                    (Hemiptera: Cicadellidae)"],"prefix":"10.1093","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9850-3404","authenticated-orcid":false,"given":"Joshua","family":"Molligan","sequence":"first","affiliation":[{"name":"D\u00e9partement de Phytologie, Facult\u00e9 des Sciences de l'agriculture et de L'alimentation, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"Centre de Recherche et D'innovation sur les V\u00e9g\u00e9taux, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"Institute de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"L\u2019Institute EDS, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8769-9242","authenticated-orcid":false,"given":"Florent","family":"Sylvestre","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Phytologie, Facult\u00e9 des Sciences de l'agriculture et de L'alimentation, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"Centre de Recherche et D'innovation sur les V\u00e9g\u00e9taux, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"Institute de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"L\u2019Institute EDS, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3708-8558","authenticated-orcid":false,"given":"Edel","family":"P\u00e9rez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Phytologie, Facult\u00e9 des Sciences de l'agriculture et de L'alimentation, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"Centre de Recherche et D'innovation sur les V\u00e9g\u00e9taux, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"Institute de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]},{"name":"L\u2019Institute EDS, Universit\u00e9 Laval , Qu\u00e9bec City, Qu\u00e9bec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"2026091107170271900_evag207-B1","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1186\/s13059-024-03198-7","article-title":"Rapid and sensitive detection of genome contamination at scale with FCS-GX","volume":"25","author":"Astashyn","year":"2024","journal-title":"Genome Biol"},{"key":"2026091107170271900_evag207-B2","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/0022-1910(88)90045-5","article-title":"Sensory systems and behaviours which mediate hemipteran plant-feeding: a taxonomic overview","volume":"34","author":"Backus","year":"1988","journal-title":"J Insect Physiol"},{"key":"2026091107170271900_evag207-B3","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1093\/ee\/18.3.473","article-title":"Comparison of feeding behavior of the potato leafhopper Empoasca fabae (Homoptera: Cicadellidae) 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Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/gbe\/advance-article-pdf\/doi\/10.1093\/gbe\/evag207\/70682007\/evag207.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/gbe\/article-pdf\/18\/9\/evag207\/70682007\/evag207.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/gbe\/article-pdf\/18\/9\/evag207\/70682007\/evag207.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:17:13Z","timestamp":1789125433000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/gbe\/article\/doi\/10.1093\/gbe\/evag207\/8763536"}},"subtitle":[],"editor":[{"given":"John","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,2]]}},"URL":"https:\/\/doi.org\/10.1093\/gbe\/evag207","relation":{},"ISSN":["1759-6653"],"issn-type":[{"value":"1759-6653","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,18]]},"article-number":"evag207"}}