{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T15:12:01Z","timestamp":1789744321793,"version":"4.0.1"},"reference-count":127,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":9,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alternative splicing (AS) promotes phenotypic plasticity to adverse conditions by altering the transcripts of genes directly involved in adaptation to those conditions. Whether changes in AS occur in a few select genes or at a larger scale is unclear. Insect pests are regularly exposed to xenobiotic stress from insecticide applications and thus develop resistance. Here, we used a genome-wide approach to show that the fruit pest Drosophila suzukii, resistant to multiple insecticides, exhibits aberrant splicing in many classes of genes, including those involved in general stress response and processes previously implicated in insecticide resistance, such as metabolic and cuticular pathways. Our results suggest that resistance is not simply a consequence of broad, transcriptome-wide increases in AS events. Instead, the specific classes of genes that are differentially spliced are better determinants of insecticide resistance. Furthermore, we observe that sublethal insecticide exposure promotes AS events even in the absence of substantial differential gene expression. Based on our results, we propose that genetic accommodation could have led to the development of insecticide resistance in D. suzukii, in which repeated insecticide exposure alters the regulation of environmentally induced AS events, ultimately leading to genetic assimilation. This leads to elevated AS of specific transcripts, a phenomenon normally induced by insecticide exposure, potentially becoming genetically encoded and constitutively expressed, even in the absence of insecticide exposure. In summary, this study provides insights into the role of AS in enabling insects to diversify genome function to survive acute insecticide treatment and to develop xenobiotic resistance upon prolonged exposure.<\/jats:p>","DOI":"10.1093\/gbe\/evag227","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:51:28Z","timestamp":1788616288000},"source":"Crossref","is-referenced-by-count":0,"title":["Changes in Alternative Splicing are Associated with Insecticide Response and Resistance"],"prefix":"10.1093","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5167-314X","authenticated-orcid":false,"given":"Christine A","family":"Tabuloc","sequence":"first","affiliation":[{"name":"Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis , Davis, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2604-156X","authenticated-orcid":false,"given":"Sergio","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis , Davis, CA ,","place":["USA"]},{"name":"Department of Integrative Physiology and Neuroscience, College of Veterinary Medicine, Washington State University , Pullman, WA ,","place":["USA"]},{"name":"Center for Reproductive Biology, Washington State University , Pullman, WA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2927-5019","authenticated-orcid":false,"given":"Curtis R","family":"Carlson","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis , Davis, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7438-6255","authenticated-orcid":false,"given":"Hongtao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Plant Biology, College of Biological Sciences, University of California Davis , Davis, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6604-1284","authenticated-orcid":false,"given":"Frank G","family":"Zalom","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis , Davis, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7613-8127","authenticated-orcid":false,"given":"Joanna C","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis , Davis, CA ,","place":["USA"]},{"name":"Department of Neurobiology, Physiology, and Behavior, College of Biological Sciences, University of California Davis , Davis, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"2026091810534288000_evag227-B1","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.ecoenv.2019.04.030","article-title":"Organochlorine and organophosphorous pesticides may induce colorectal cancer; a case-control study","volume":"178","author":"Abolhassani","year":"2019","journal-title":"Ecotoxicol Environ Saf"},{"key":"2026091810534288000_evag227-B2","doi-asserted-by":"publisher","first-page":"146432","DOI":"10.1016\/j.scitotenv.2021.146432","article-title":"Sublethal agrochemical exposures can alter honey bees\u2019 and Neotropical stingless bees\u2019 color preferences, respiration rates, and locomotory responses","volume":"779","author":"Almeida","year":"2021","journal-title":"Sci Total Environ"},{"key":"2026091810534288000_evag227-B3","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1186\/s40246-024-00694-6","article-title":"Global transcriptome modulation by xenobiotics: the role of alternative splicing in adaptive responses to chemical exposures","volume":"18","author":"Annalora","year":"2024","journal-title":"Hum Genomics"},{"key":"2026091810534288000_evag227-B4","doi-asserted-by":"publisher","first-page":"116280","DOI":"10.1016\/j.taap.2022.116280","article-title":"A systematic review of mechanistic studies on the relationship between pesticide exposure and cancer induction","volume":"456","author":"Ataei","year":"2022","journal-title":"Toxicol Appl Pharmacol"},{"key":"2026091810534288000_evag227-B5","doi-asserted-by":"publisher","first-page":"5692","DOI":"10.1111\/mec.13882","article-title":"Insecticide resistance mediated by an exon skipping event","volume":"25","author":"Berger","year":"2016","journal-title":"Mol Ecol"},{"key":"2026091810534288000_evag227-B6","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B7","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/s41571-020-0350-x","article-title":"Roles and mechanisms of alternative splicing in cancer\u2014implications for care","volume":"17","author":"Bonnal","year":"2020","journal-title":"Nat Rev Clin Oncol"},{"key":"2026091810534288000_evag227-B8","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1038\/s41580-023-00624-9","article-title":"The molecular basis of translation initiation and its regulation in eukaryotes","volume":"25","author":"Brito Querido","year":"2024","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026091810534288000_evag227-B9","doi-asserted-by":"publisher","first-page":"lqaa108","DOI":"10.1093\/nargab\/lqaa108","article-title":"BRAKER2: automatic eukaryotic genome annotation with GeneMark-EP+ and AUGUSTUS supported by a protein database","volume":"3","author":"Br\u016fna","year":"2021","journal-title":"NAR Genom Bioinform"},{"key":"2026091810534288000_evag227-B10","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1101\/gr.278373.123","article-title":"GeneMark-ETP significantly improves the accuracy of automatic annotation of large eukaryotic genomes","volume":"34","author":"Bruna","year":"2024","journal-title":"Genome Res"},{"key":"2026091810534288000_evag227-B11","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and sensitive protein alignment using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat Methods"},{"key":"2026091810534288000_evag227-B12","doi-asserted-by":"publisher","first-page":"1626","DOI":"10.1603\/0022-0493-96.6.1626","article-title":"Haplodiploidy, sex, and the evolution of pesticide resistance","volume":"96","author":"Carri\u00e8re","year":"2003","journal-title":"J Econ Entomol"},{"key":"2026091810534288000_evag227-B13","doi-asserted-by":"publisher","first-page":"9077","DOI":"10.1093\/nar\/gkab688","article-title":"tRNAscan-SE 2.0: improved detection and functional classification of transfer RNA genes","volume":"49","author":"Chan","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2026091810534288000_evag227-B14","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1016\/j.jmb.2019.03.001","article-title":"RNA splicing: a new paradigm in host-pathogen interactions","volume":"431","author":"Chauhan","year":"2019","journal-title":"J Mol Biol"},{"key":"2026091810534288000_evag227-B15","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B16","doi-asserted-by":"publisher","first-page":"RP110625","DOI":"10.7554\/eLife.110625.1","article-title":"Transposons contribute to splice-isoform diversity in the Drosophila brain","volume":"15","author":"Choucri","year":"2026","journal-title":"eLife"},{"key":"2026091810534288000_evag227-B17","doi-asserted-by":"publisher","first-page":"20190342","DOI":"10.1098\/rstb.2019.0342","article-title":"An atlas of transposable element-derived alternative splicing in cancer","volume":"375","author":"Clayton","year":"2020","journal-title":"Philos Trans R Soc B Biol Sci"},{"key":"2026091810534288000_evag227-B18","doi-asserted-by":"publisher","first-page":"iyad034","DOI":"10.1093\/genetics\/iyad034","article-title":"Selection and geography shape male reproductive tract transcriptomes in Drosophila melanogaster","volume":"224","author":"Cridland","year":"2023","journal-title":"Genetics"},{"key":"2026091810534288000_evag227-B19","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1038\/s41580-021-00356-8","article-title":"Intracellular mRNA transport and localized translation","volume":"22","author":"Das","year":"2021","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2026091810534288000_evag227-B20","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s12199-014-0425-3","article-title":"Insecticide residues in soil, water, and eggplant fruits and farmers\u2019 health effects due to exposure to pesticides","volume":"20","author":"Del Prado-Lu","year":"2015","journal-title":"Environ Health Prev Med"},{"key":"2026091810534288000_evag227-B21","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1038\/s41418-018-0231-3","article-title":"Function, clinical application, and strategies of Pre-mRNA splicing in cancer","volume":"26","author":"Di","year":"2019","journal-title":"Cell Death Differ"},{"key":"2026091810534288000_evag227-B22","doi-asserted-by":"publisher","first-page":"2918","DOI":"10.1002\/ps.5880","article-title":"Insecticide residue longevity for on-site screening of Drosophila suzukii (Matsumura) resistance","volume":"76","author":"Disi","year":"2020","journal-title":"Pest Manag Sci"},{"key":"2026091810534288000_evag227-B23","doi-asserted-by":"publisher","first-page":"794","DOI":"10.3390\/insects12090794","article-title":"Laboratory selection and assessment of resistance risk in Drosophila suzukii (Diptera: Drosophilidae) to spinosad and malathion","volume":"12","author":"Disi","year":"2021","journal-title":"Insects"},{"key":"2026091810534288000_evag227-B24","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ibmb.2014.03.012","article-title":"Molecular biology of insect sodium channels and pyrethroid resistance","volume":"50","author":"Dong","year":"2014","journal-title":"Insect Biochem Mol Biol"},{"key":"2026091810534288000_evag227-B26","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1126\/science.adp4671","article-title":"Rapid butterfly declines across the United States during the 21st century","volume":"387","author":"Edwards","year":"2025","journal-title":"Science"},{"key":"2026091810534288000_evag227-B27","doi-asserted-by":"publisher","first-page":"125006","DOI":"10.1016\/j.envpol.2024.125006","article-title":"Trace organic contaminants in U.S. national park surface waters: prevalence and ecological context","volume":"362","author":"Elliott","year":"2024","journal-title":"Environ Pollut Barking Essex 1987"},{"key":"2026091810534288000_evag227-B28","doi-asserted-by":"publisher","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B29","doi-asserted-by":"publisher","first-page":"141895","DOI":"10.1016\/j.scitotenv.2020.141895","article-title":"Pesticides and their degradates in groundwater reflect past use and current management strategies, Long Island, New York, USA","volume":"752","author":"Fisher","year":"2021","journal-title":"Sci Total Environ"},{"key":"2026091810534288000_evag227-B30","doi-asserted-by":"publisher","first-page":"9451","DOI":"10.1073\/pnas.1921046117","article-title":"RepeatModeler2 for automated genomic discovery of transposable element families","volume":"117","author":"Flynn","year":"2020","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026091810534288000_evag227-B31","doi-asserted-by":"publisher","first-page":"113569","DOI":"10.1016\/j.ijheh.2020.113569","article-title":"An epigenome-wide association study of ambient pyrethroid pesticide exposures in California's central valley","volume":"229","author":"Furlong","year":"2020","journal-title":"Int J Hyg Environ Health"},{"key":"2026091810534288000_evag227-B32","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1101\/gr.278090.123","article-title":"BRAKER3: fully automated genome annotation using RNA-seq and protein evidence with GeneMark-ETP, AUGUSTUS, and TSEBRA","volume":"34","author":"Gabriel","year":"2024","journal-title":"Genome Res"},{"key":"2026091810534288000_evag227-B33","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1126\/science.ado0251","article-title":"Pervasive sublethal effects of agrochemicals on insects at environmentally relevant concentrations","volume":"386","author":"Gandara","year":"2024","journal-title":"Science"},{"key":"2026091810534288000_evag227-B34","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.1093\/jee\/toac118","article-title":"Characterization of field-derived Drosophila suzukii (Diptera: Drosophilidae) resistance to pyrethroids in California berry production","volume":"115","author":"Ganjisaffar","year":"2022","journal-title":"J Econ Entomol"},{"key":"2026091810534288000_evag227-B35","doi-asserted-by":"publisher","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B36","doi-asserted-by":"publisher","first-page":"e0240950","DOI":"10.1371\/journal.pone.0240950","article-title":"Acute exposure to sublethal doses of neonicotinoid insecticides increases heat tolerance in honey bees","volume":"17","author":"Gonzalez","year":"2022","journal-title":"PLoS One"},{"key":"2026091810534288000_evag227-B37","doi-asserted-by":"publisher","first-page":"102304","DOI":"10.1016\/j.ceb.2023.102304","article-title":"Regulation of protein synthesis and stability by mechanical cues and its implications in cancer","volume":"86","author":"G\u00f6ransson","year":"2024","journal-title":"Curr Opin Cell Biol"},{"key":"2026091810534288000_evag227-B38","doi-asserted-by":"publisher","first-page":"2630","DOI":"10.1093\/nar\/gkn105","article-title":"A space-efficient and accurate method for mapping and aligning cDNA sequences onto genomic sequence","volume":"36","author":"Gotoh","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"2026091810534288000_evag227-B39","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1093\/genetics\/160.4.1519","article-title":"Novel putative nicotinic acetylcholine receptor subunit genes, Dalpha5, Dalpha6 and Dalpha7, in Drosophila melanogaster identify a new and highly conserved target of adenosine deaminase acting on RNA-mediated A-to-I pre-mRNA editing","volume":"160","author":"Grauso","year":"2002","journal-title":"Genetics"},{"key":"2026091810534288000_evag227-B40","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1002\/ps.5240","article-title":"Identification and risk assessment of spinosad resistance in a California population of Drosophila suzukii","volume":"75","author":"Gress","year":"2019","journal-title":"Pest Manag Sci"},{"key":"2026091810534288000_evag227-B41","doi-asserted-by":"publisher","first-page":"e0270747","DOI":"10.1371\/journal.pone.0270747","article-title":"Development and validation of a larval bioassay and selection protocol for insecticide resistance in Drosophila suzukii","volume":"17","author":"Gress","year":"2022","journal-title":"PLoS One"},{"key":"2026091810534288000_evag227-B42","doi-asserted-by":"publisher","first-page":"194405","DOI":"10.1016\/j.bbagrm.2019.07.006","article-title":"Repeat-associated RNA structure and aberrant splicing","volume":"1862","author":"Hale","year":"2019","journal-title":"Biochim Biophys Acta BBA\u2014Gene Regul Mech"},{"key":"2026091810534288000_evag227-B43","doi-asserted-by":"publisher","first-page":"e68472","DOI":"10.1371\/journal.pone.0068472","article-title":"Integrating circadian activity and gene expression profiles to predict chronotoxicity of Drosophila suzukii response to insecticides","volume":"8","author":"Hamby","year":"2013","journal-title":"PLoS One"},{"key":"2026091810534288000_evag227-B44","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1111\/1755-0998.13106","article-title":"EnTAP: bringing faster and smarter functional annotation to non-model eukaryotic transcriptomes","volume":"20","author":"Hart","year":"2020","journal-title":"Mol Ecol Resour"},{"key":"2026091810534288000_evag227-B45","doi-asserted-by":"publisher","first-page":"6549","DOI":"10.1093\/nar\/gkw533","article-title":"Splice-switching antisense oligonucleotides as therapeutic drugs","volume":"44","author":"Havens","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"2026091810534288000_evag227-B46","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.7150\/ijbs.4966","article-title":"Multiple sodium channel variants in the mosquito Culex quinquefasciatus","volume":"8","author":"He","year":"2012","journal-title":"Int J Biol Sci"},{"key":"2026091810534288000_evag227-B47","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1098\/rstb.1998.0320","article-title":"The role of gene splicing, gene amplification and regulation in mosquito insecticide resistance","volume":"353","author":"Hemingway","year":"1998","journal-title":"Philos Trans R Soc B Biol Sci"},{"key":"2026091810534288000_evag227-B48","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1093\/bioinformatics\/btv661","article-title":"BRAKER1: unsupervised RNA-Seq-based genome annotation with GeneMark-ET and AUGUSTUS: Table 1","volume":"32","author":"Hoff","year":"2016","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B49","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-1-4939-9173-0_5","article-title":"Whole-genome annotation with BRAKER","volume":"1962","author":"Hoff","year":"2019","journal-title":"Methods Mol Biol (Clifton, N.J.)"},{"key":"2026091810534288000_evag227-B50","doi-asserted-by":"publisher","first-page":"R240","DOI":"10.1152\/ajpregu.00012.2019","article-title":"Mild hypothermia causes a shift in the alternative splicing of cold-inducible RNA-binding protein transcripts in Syrian hamsters","volume":"317","author":"Horii","year":"2019","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"2026091810534288000_evag227-B51","doi-asserted-by":"publisher","first-page":"e161","DOI":"10.1093\/nar\/gks708","article-title":"Benchmarking spliced alignment programs including Spaln2, an extended version of Spaln that incorporates additional species-specific features","volume":"40","author":"Iwata","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"2026091810534288000_evag227-B52","doi-asserted-by":"publisher","first-page":"18065","DOI":"10.1038\/s41598-021-96829-z","article-title":"PacBio sequencing output increased through uniform and directional fivefold concatenation","volume":"11","author":"Kanwar","year":"2021","journal-title":"Sci Rep"},{"key":"2026091810534288000_evag227-B53","doi-asserted-by":"publisher","first-page":"170113","DOI":"10.1016\/j.scitotenv.2024.170113","article-title":"Pesticides: an alarming detrimental to health and environment","volume":"915","author":"Kaur","year":"2024","journal-title":"Sci Total Environ"},{"key":"2026091810534288000_evag227-B54","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/j.jbior.2012.04.003","article-title":"Pre-mRNA splicing: role of epigenetics and implications in disease","volume":"52","author":"Khan","year":"2012","journal-title":"Adv Biol Regul"},{"key":"2026091810534288000_evag227-B55","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat Biotechnol"},{"key":"2026091810534288000_evag227-B56","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1080\/10599240902824042","article-title":"Oxidative stress and DNA damage in agricultural workers","volume":"14","author":"Kisby","year":"2009","journal-title":"J Agromedicine"},{"key":"2026091810534288000_evag227-B57","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1002\/ps.3395","article-title":"Fitness costs associated with insecticide resistance","volume":"68","author":"Kliot","year":"2012","journal-title":"Pest Manag Sci"},{"key":"2026091810534288000_evag227-B58","doi-asserted-by":"publisher","first-page":"3211","DOI":"10.1093\/bioinformatics\/bts611","article-title":"SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data","volume":"28","author":"Kopylova","year":"2012","journal-title":"Bioinforma Oxf Engl"},{"key":"2026091810534288000_evag227-B59","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1186\/s13059-019-1910-1","article-title":"Transcriptome assembly from long-read RNA-seq alignments with StringTie2","volume":"20","author":"Kovaka","year":"2019","journal-title":"Genome Biol"},{"key":"2026091810534288000_evag227-B60","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.1007\/s10646-022-02600-z","article-title":"Persistence, leaching and associated toxicity risks of insecticide pyriproxyfen in soil ecosystem","volume":"31","author":"Kumari","year":"2022","journal-title":"Ecotoxicol Lond Engl"},{"key":"2026091810534288000_evag227-B61","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1038\/s41586-024-08552-0","article-title":"Tumour-wide RNA splicing aberrations generate actionable public neoantigens","volume":"639","author":"Kwok","year":"2025","journal-title":"Nature"},{"key":"2026091810534288000_evag227-B62","doi-asserted-by":"publisher","first-page":"3100","DOI":"10.1093\/nar\/gkm160","article-title":"RNAmmer: consistent and rapid annotation of ribosomal RNA genes","volume":"35","author":"Lagesen","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"2026091810534288000_evag227-B63","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.tplants.2017.09.019","article-title":"Alternative splicing control of abiotic stress responses","volume":"23","author":"Laloum","year":"2018","journal-title":"Trends Plant Sci"},{"key":"2026091810534288000_evag227-B64","doi-asserted-by":"publisher","first-page":"eadf2572","DOI":"10.1126\/science.adf2572","article-title":"Spillover effects of organic agriculture on pesticide use on nearby fields","volume":"383","author":"Larsen","year":"2024","journal-title":"Science"},{"key":"2026091810534288000_evag227-B65","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1126\/science.1241097","article-title":"Regulation of temperature-responsive flowering by MADS-box transcription factor repressors","volume":"342","author":"Lee","year":"2013","journal-title":"Science"},{"key":"2026091810534288000_evag227-B66","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"2026091810534288000_evag227-B67","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.cell.2010.11.056","article-title":"Epigenetics in alternative pre-mRNA splicing","volume":"144","author":"Luco","year":"2011","journal-title":"Cell"},{"key":"2026091810534288000_evag227-B68","doi-asserted-by":"publisher","first-page":"238","DOI":"10.3390\/insects15040238","article-title":"miRNA dynamics for pest management: implications in insecticide resistance","volume":"15","author":"Mahalle","year":"2024","journal-title":"Insects"},{"key":"2026091810534288000_evag227-B69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41540-022-00225-w","article-title":"Transcriptome analysis of clock disrupted cancer cells reveals differential alternative splicing of cancer hallmarks genes","volume":"8","author":"Malhan","year":"2022","journal-title":"Npj Syst Biol Appl"},{"key":"2026091810534288000_evag227-B70","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1186\/s13059-020-02258-y","article-title":"Alternative splicing landscapes in Arabidopsis thaliana across tissues and stress conditions highlight major functional differences with animals","volume":"22","author":"Mart\u00edn","year":"2021","journal-title":"Genome Biol"},{"key":"2026091810534288000_evag227-B71","doi-asserted-by":"publisher","first-page":"e44642","DOI":"10.7554\/eLife.44642","article-title":"Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila. Ramaswami M, Calabrese RL, editors","volume":"8","author":"Martin Anduaga","year":"2019","journal-title":"eLife"},{"key":"2026091810534288000_evag227-B72","doi-asserted-by":"publisher","first-page":"4252","DOI":"10.1093\/molbev\/msab155","article-title":"The worldwide invasion of Drosophila suzukii is accompanied by a large increase of transposable element load and a small number of putatively adaptive insertions","volume":"38","author":"M\u00e9rel","year":"2021","journal-title":"Mol Biol Evol"},{"key":"2026091810534288000_evag227-B73","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1111\/1744-7917.12498","article-title":"High throughput sequencing reveals Drosophila suzukii responses to insecticides","volume":"25","author":"Mishra","year":"2018","journal-title":"Insect Sci"},{"key":"2026091810534288000_evag227-B74","doi-asserted-by":"publisher","first-page":"1044980","DOI":"10.3389\/fgene.2022.1044980","article-title":"Epigenetic regulations as drivers of insecticide resistance and resilience to climate change in arthropod pests","volume":"13","author":"Mogilicherla","year":"2023","journal-title":"Front Genet"},{"key":"2026091810534288000_evag227-B75","doi-asserted-by":"publisher","first-page":"10305","DOI":"10.1073\/pnas.1803880115","article-title":"Glyphosate perturbs the gut microbiota of honey bees","volume":"115","author":"Motta","year":"2018","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026091810534288000_evag227-B76","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.tiv.2017.06.013","article-title":"Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro","volume":"44","author":"Navarrete-Meneses","year":"2017","journal-title":"Toxicol Vitro Int J Publ Assoc BIBRA"},{"key":"2026091810534288000_evag227-B77","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s13100-025-00341-4","article-title":"Alternative splicing of transposable elements in human breast cancer","volume":"16","author":"Nesta","year":"2025","journal-title":"Mob DNA"},{"key":"2026091810534288000_evag227-B78","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.ydbio.2025.04.011","article-title":"Genetic assimilation, robustness and plasticity are key processes in the development and evolution of novel traits","volume":"523","author":"Nijhout","year":"2025","journal-title":"Dev Biol"},{"key":"2026091810534288000_evag227-B79","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/bs.ctdb.2020.11.006","article-title":"Genetic assimilation and accommodation: models and mechanisms","volume":"141","author":"Nijhout","year":"2021","journal-title":"Curr Top Dev Biol."},{"key":"2026091810534288000_evag227-B80","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/nature08909","article-title":"Expansion of the eukaryotic proteome by alternative splicing","volume":"463","author":"Nilsen","year":"2010","journal-title":"Nature"},{"key":"2026091810534288000_evag227-B81","doi-asserted-by":"publisher","first-page":"iyad211","DOI":"10.1093\/genetics\/iyad211","article-title":"FlyBase: updates to the Drosophila genes and genomes database","volume":"227","author":"\u00d6zt\u00fcrk-\u00c7olak","year":"2024","journal-title":"Genetics"},{"key":"2026091810534288000_evag227-B82","doi-asserted-by":"publisher","first-page":"20170073","DOI":"10.1098\/rstb.2017.0073","article-title":"Bioinformatics challenges and perspectives when studying the effect of epigenetic modifications on alternative splicing","volume":"373","author":"Pacini","year":"2018","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"2026091810534288000_evag227-B83","doi-asserted-by":"publisher","first-page":"11227","DOI":"10.1038\/s41598-020-67373-z","article-title":"Near-chromosome level genome assembly of the fruit pest Drosophila suzukii using long-read sequencing","volume":"10","author":"Paris","year":"2020","journal-title":"Sci Rep"},{"key":"2026091810534288000_evag227-B84","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat Methods"},{"key":"2026091810534288000_evag227-B85","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1016\/j.scitotenv.2018.07.143","article-title":"Organophosphate pesticide exposure and differential genome-wide DNA methylation","volume":"645","author":"Paul","year":"2018","journal-title":"Sci Total Environ"},{"key":"2026091810534288000_evag227-B86","doi-asserted-by":"publisher","first-page":"ISCB Comm J-304","DOI":"10.12688\/f1000research.23297.2","article-title":"GFF utilities: GffRead and GffCompare","volume":"9","author":"Pertea","year":"2020","journal-title":"F1000Res."},{"key":"2026091810534288000_evag227-B87","doi-asserted-by":"publisher","first-page":"2362","DOI":"10.1242\/jeb.02070","article-title":"Phenotypic plasticity and evolution by genetic assimilation","volume":"209","author":"Pigliucci","year":"2006","journal-title":"J Exp Biol"},{"key":"2026091810534288000_evag227-B88","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1038\/nature12633","article-title":"Temperature-dependent regulation of flowering by antagonistic FLM variants","volume":"503","author":"Pos\u00e9","year":"2013","journal-title":"Nature"},{"key":"2026091810534288000_evag227-B89","doi-asserted-by":"publisher","first-page":"841","DOI":"10.3945\/jn.114.203216","article-title":"Role of precursor mRNA splicing in nutrient-induced alterations in gene expression and metabolism1, 2, 3","volume":"145","author":"Ravi","year":"2015","journal-title":"J Nutr"},{"key":"2026091810534288000_evag227-B90","doi-asserted-by":"publisher","first-page":"100728","DOI":"10.1016\/j.drup.2020.100728","article-title":"The role of alternative splicing in cancer: from oncogenesis to drug resistance","volume":"53","author":"Sciarrillo","year":"2020","journal-title":"Drug Resist Updat Rev Comment Antimicrob Anticancer Chemother"},{"key":"2026091810534288000_evag227-B91","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1186\/1471-2164-8-324","article-title":"The nicotinic acetylcholine receptor gene family of the silkworm, Bombyx mori","volume":"8","author":"Shao","year":"2007","journal-title":"BMC Genomics"},{"key":"2026091810534288000_evag227-B92","doi-asserted-by":"publisher","first-page":"e0248749","DOI":"10.1371\/journal.pone.0248749","article-title":"Full length sequencing reveals novel transcripts of detoxification genes along with related alternative splicing events and lncRNAs in Phyllotreta striolata","volume":"16","author":"Shen","year":"2021","journal-title":"PLoS One"},{"key":"2026091810534288000_evag227-B93","doi-asserted-by":"publisher","first-page":"7599","DOI":"10.3390\/ijms21207599","article-title":"Temperature-dependent alternative splicing of precursor mRNAs and its biological significance: a review focused on post-transcriptional regulation of a cold shock protein gene in hibernating mammals","volume":"21","author":"Shiina","year":"2020","journal-title":"Int J Mol Sci"},{"key":"2026091810534288000_evag227-B94","doi-asserted-by":"publisher","first-page":"130661","DOI":"10.1016\/j.bbagen.2024.130661","article-title":"Epigenetic regulation of abiotic stress responses in plants","volume":"1868","author":"Shilpa","year":"2024","journal-title":"Biochim Biophys Acta BBA\u2014Gen Subj"},{"key":"2026091810534288000_evag227-B95","doi-asserted-by":"publisher","first-page":"1112278","DOI":"10.3389\/fphys.2022.1112278","article-title":"Insights into insecticide-resistance mechanisms in invasive species: challenges and control strategies","volume":"13","author":"Siddiqui","year":"2023","journal-title":"Front Physiol"},{"key":"2026091810534288000_evag227-B96","doi-asserted-by":"publisher","first-page":"1928","DOI":"10.1111\/mec.16377","article-title":"The importance of alternative splicing in adaptive evolution","volume":"31","author":"Singh","year":"2022","journal-title":"Mol Ecol"},{"key":"2026091810534288000_evag227-B97","doi-asserted-by":"publisher","first-page":"2586","DOI":"10.1111\/ele.13873","article-title":"Field-realistic neonicotinoid exposure has sub-lethal effects on non-Apis bees: a meta-analysis","volume":"24","author":"Siviter","year":"2021","journal-title":"Ecol Lett"},{"key":"2026091810534288000_evag227-B98","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1093\/bioinformatics\/btn013","article-title":"Using native and syntenically mapped cDNA alignments to improve de novo gene finding","volume":"24","author":"Stanke","year":"2008","journal-title":"Bioinformatics"},{"key":"2026091810534288000_evag227-B99","doi-asserted-by":"publisher","first-page":"e2109909118","DOI":"10.1073\/pnas.2109909118","article-title":"Past insecticide exposure reduces bee reproduction and population growth rate","volume":"118","author":"Stuligross","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091810534288000_evag227-B100","doi-asserted-by":"publisher","first-page":"103474","DOI":"10.1016\/j.etap.2020.103474","article-title":"Exposure to organophosphorus insecticides and increased risks of health and cancer in US women","volume":"80","author":"Sun","year":"2020","journal-title":"Environ Toxicol Pharmacol"},{"key":"2026091810534288000_evag227-B101","doi-asserted-by":"publisher","first-page":"19867","DOI":"10.1038\/s41598-024-70037-x","article-title":"Transcriptome analysis of Drosophila suzukii reveals molecular mechanisms conferring pyrethroid and spinosad resistance","volume":"14","author":"Tabuloc","year":"2024","journal-title":"Sci Rep"},{"key":"2026091810534288000_evag227-B102","doi-asserted-by":"publisher","first-page":"e78","DOI":"10.1093\/nar\/gkv227","article-title":"Identification of protein coding regions in RNA transcripts","volume":"43","author":"Tang","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"2026091810534288000_evag227-B103","doi-asserted-by":"publisher","first-page":"4.10.1","DOI":"10.1002\/0471250953.bi0410s25","article-title":"Using RepeatMasker to identify repetitive elements in genomic sequences","volume":"25","author":"Tarailo-Graovac","year":"2009","journal-title":"Curr Protoc Bioinforma"},{"key":"2026091810534288000_evag227-B104","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s13059-018-1417-1","article-title":"SUPPA2: fast, accurate, and uncertainty-aware differential splicing analysis across multiple conditions","volume":"19","author":"Trincado","year":"2018","journal-title":"Genome Biol"},{"key":"2026091810534288000_evag227-B105","doi-asserted-by":"publisher","first-page":"2961","DOI":"10.1038\/s41598-019-38681-w","article-title":"Multiple mutations in the nicotinic acetylcholine receptor Cc\u03b16 gene associated with resistance to spinosad in medfly","volume":"9","author":"Ure\u00f1a","year":"2019","journal-title":"Sci Rep"},{"key":"2026091810534288000_evag227-B106","doi-asserted-by":"publisher","first-page":"1782","DOI":"10.1002\/ps.5341","article-title":"Development of a rapid assessment method for detecting insecticide resistance in spotted wing Drosophila (Drosophila suzukii Matsumura)","volume":"75","author":"Van Timmeren","year":"2019","journal-title":"Pest Manag Sci"},{"key":"2026091810534288000_evag227-B107","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1002\/ps.4702","article-title":"Baseline susceptibility of spotted wing Drosophila (Drosophila suzukii) to four key insecticide classes","volume":"74","author":"Van Timmeren","year":"2018","journal-title":"Pest Manag Sci"},{"key":"2026091810534288000_evag227-B108","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.tree.2021.11.010","article-title":"The role of alternative splicing in adaptation and evolution","volume":"37","author":"Verta","year":"2022","journal-title":"Trends Ecol Evol"},{"key":"2026091810534288000_evag227-B109","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1038\/150563a0","article-title":"Canalization of development and the inheritance of acquired characters","volume":"150","author":"Waddington","year":"1942","journal-title":"Nature"},{"key":"2026091810534288000_evag227-B110","doi-asserted-by":"publisher","first-page":"118","DOI":"10.2307\/2405747","article-title":"Genetic assimilation of an acquired character","volume":"7","author":"Waddington","year":"1953","journal-title":"Evolution"},{"key":"2026091810534288000_evag227-B111","doi-asserted-by":"publisher","first-page":"154697","DOI":"10.1016\/j.scitotenv.2022.154697","article-title":"Pesticide exposure of wild bees and honey bees foraging from field border flowers in intensively managed agriculture areas","volume":"831","author":"Ward","year":"2022","journal-title":"Sci Total Environ"},{"key":"2026091810534288000_evag227-B112","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1111\/1744-7917.13166","article-title":"Alternative splicing of a carboxyl\/choline esterase gene enhances the fenpropathrin tolerance of Tetranychus cinnabarinus","volume":"30","author":"Wei","year":"2022","journal-title":"Insect Sci"},{"key":"2026091810534288000_evag227-B113","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195122343.001.0001","volume-title":"Developmental plasticity and evolution","author":"West-Eberhard","year":"2003"},{"key":"2026091810534288000_evag227-B114","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1002\/jez.b.21071","article-title":"Phenotypic accommodation: adaptive innovation due to developmental plasticity","volume":"304B","author":"West-Eberhard","year":"2005","journal-title":"J Exp Zoolog B Mol Dev Evol"},{"key":"2026091810534288000_evag227-B115","doi-asserted-by":"publisher","first-page":"6543","DOI":"10.1073\/pnas.0501844102","article-title":"Developmental plasticity and the origin of species differences","volume":"102","author":"West-Eberhard","year":"2005","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026091810534288000_evag227-B116","doi-asserted-by":"publisher","first-page":"15527","DOI":"10.1074\/jbc.M009393200","article-title":"A novel family of chitin-binding proteins from insect type 2 peritrophic matrix: cDNA SEQUENCES, CHITIN BINDING ACTIVITY, AND CELLULAR LOCALIZATION*","volume":"276","author":"Wijffels","year":"2001","journal-title":"J Biol Chem"},{"key":"2026091810534288000_evag227-B117","doi-asserted-by":"publisher","first-page":"e2221692120","DOI":"10.1073\/pnas.2221692120","article-title":"Climate, pesticides, and landcover drive declines of the western bumble bee","volume":"120","author":"Williams","year":"2023","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091810534288000_evag227-B118","doi-asserted-by":"publisher","first-page":"2829","DOI":"10.3390\/ijms20112829","article-title":"Insect ATP-binding cassette (ABC) transporters: roles in xenobiotic detoxification and Bt insecticidal activity","volume":"20","author":"Wu","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2026091810534288000_evag227-B119","doi-asserted-by":"publisher","first-page":"1540","DOI":"10.1093\/plcell\/koad197","article-title":"What, where, and how: regulation of translation and the translational landscape in plants","volume":"36","author":"Wu","year":"2024","journal-title":"Plant Cell"},{"key":"2026091810534288000_evag227-B120","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1002\/ps.4340","article-title":"Expression pattern and pharmacological characterisation of two novel alternative splice variants of the glutamate-gated chloride channel in the small brown planthopper Laodelphax striatellus","volume":"73","author":"Wu","year":"2017","journal-title":"Pest Manag Sci"},{"key":"2026091810534288000_evag227-B121","doi-asserted-by":"publisher","first-page":"516269","DOI":"10.3389\/fgene.2020.516269","article-title":"PacBio long reads improve metagenomic assemblies, gene catalogs, and genome binning","volume":"11","author":"Xie","year":"2020","journal-title":"Front Genet"},{"key":"2026091810534288000_evag227-B122","doi-asserted-by":"publisher","first-page":"2034","DOI":"10.1038\/s41593-025-02039-5","article-title":"Aberrant splicing exonizes C9orf72 repeat expansion in ALS\/FTD","volume":"28","author":"Yang","year":"2025","journal-title":"Nat Neurosci"},{"key":"2026091810534288000_evag227-B123","doi-asserted-by":"publisher","first-page":"eaap9927","DOI":"10.1126\/scitranslmed.aap9927","article-title":"HIV latency in isolated patient CD4+ T cells may be due to blocks in HIV transcriptional elongation, completion, and splicing","volume":"10","author":"Yukl","year":"2018","journal-title":"Sci Transl Med"},{"key":"2026091810534288000_evag227-B124","doi-asserted-by":"publisher","first-page":"112703","DOI":"10.1016\/j.envres.2022.112703","article-title":"Exposure to neonicotinoid insecticides and their characteristic metabolites: association with human liver cancer","volume":"208","author":"Zhang","year":"2022","journal-title":"Environ Res"},{"key":"2026091810534288000_evag227-B125","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.3389\/fgene.2019.01000","article-title":"The roles of four novel P450 genes in pesticides resistance in Apis cerana cerana fabricius: expression levels and detoxification efficiency","volume":"10","author":"Zhang","year":"2019","journal-title":"Front Genet"},{"key":"2026091810534288000_evag227-B126","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1038\/s41392-021-00486-7","article-title":"Alternative splicing and cancer: a systematic review","volume":"6","author":"Zhang","year":"2021","journal-title":"Signal Transduct Target Ther"},{"key":"2026091810534288000_evag227-B127","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1111\/tpj.13571","article-title":"Proteogenomic analysis reveals alternative splicing and translation as part of the abscisic acid response in Arabidopsis seedlings","volume":"91","author":"Zhu","year":"2017","journal-title":"Plant J"}],"container-title":["Genome Biology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/gbe\/advance-article-pdf\/doi\/10.1093\/gbe\/evag227\/71018194\/evag227.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/gbe\/article-pdf\/18\/9\/evag227\/71018194\/evag227.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/gbe\/article-pdf\/18\/9\/evag227\/71018194\/evag227.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:53:49Z","timestamp":1789743229000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/gbe\/article\/doi\/10.1093\/gbe\/evag227\/8789873"}},"subtitle":[],"editor":[{"given":"Josefa","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":127,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,2]]}},"URL":"https:\/\/doi.org\/10.1093\/gbe\/evag227","relation":{},"ISSN":["1759-6653"],"issn-type":[{"value":"1759-6653","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,9]]},"article-number":"evag227"}}