{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T21:37:24Z","timestamp":1648935444985},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1992,12,1]],"date-time":"1992-12-01T00:00:00Z","timestamp":723168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The Adh-2 gene of Drosophila mulleri is expressed in the larval fat body and the adult fat body and hindgut, and a 1500-bp element located 2-3 kb upstream of the Adh-2 promoter is necessary for maximal levels of transcription. Previous work demonstrated that deletion of sequences between this upstream element and the Adh-2 promoter results in Adh-2 gene expression in a novel larval tissue, the middle midgut. In this study we show that the upstream element possesses all of the characteristics of a transcriptional enhancer: its activity is independent of orientation, it acts on a heterologous promoter, and it functions at various positions both 5' and 3' to the Adh-2 gene. Full enhancer function can be localized to a 750-bp element, although other regions possess some redundant activity. The ectopic expression pattern is dependent on the proximity of at least two sequence elements. Thus, tissue-specific transcription can involve complex proximity-dependent interactions among combinations of regulatory elements.<\/jats:p>","DOI":"10.1093\/genetics\/132.4.1071","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T19:04:49Z","timestamp":1610564689000},"page":"1071-1079","source":"Crossref","is-referenced-by-count":0,"title":["Rearrangement of upstream regulatory elements leads to ectopic expression of the Drosophila mulleri Adh-2 gene."],"prefix":"10.1093","volume":"132","author":[{"given":"D","family":"Falb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Maniatis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1992,12,1]]},"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/132\/4\/1071\/34572446\/genetics1071.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/132\/4\/1071\/34572446\/genetics1071.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T19:18:42Z","timestamp":1619119122000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/132\/4\/1071\/6009164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,12,1]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/132.4.1071","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1992,12,1]]},"published":{"date-parts":[[1992,12,1]]}}}